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  • Books
  • Articles  (17)
  • Volumetrie  (14)
  • Chemical Engineering
  • Electronic structure and strongly correlated systems
  • Saccharomyces cerevisiae
  • Springer  (17)
  • 1975-1979  (17)
  • 1975  (17)
Collection
  • Books
  • Articles  (17)
Keywords
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Years
  • 1975-1979  (17)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 105 (1975), S. 187-192 
    ISSN: 1432-072X
    Keywords: Peroxisomes (microbodies) ; Saccharomyces cerevisiae ; Catalase ; Urate oxidase ; Glyoxylate cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Peroxisomes were isolated from derepressed (lactose grown) Saccharomyces cerevisiae cells following homogenization with a “Merkenschlager” cell mill (at 0°C using glass beads). Catalase and urate oxidase, along with low activities of d-amino acid oxidase and l-α-hydroxyacid oxidase (glycollate oxidase), were associated with the peroxisomes. No catalase activity was present in glucose repressed cells. When protoplasts prepared from derepressed cells were used for peroxisome isolation, catalase activity was not sedimentable through gradients. Apparently peroxisomes were destroyed as the cells became fermentative during protoplast preparation. The distribution of glyoxylate cycle enzymes was examined. Isocitrate lyase was not sedimentable, suggesting that, if the enzyme is peroxisome-associated, it is either readily released or present in a labile second class of peroxisomes. Low activities of malate dehydrogenase and citrate synthetase were found in peroxisome fractions from gradients, but may represent mitochondrial contamination. Citrate synthetase was not found associated with a low-density particle as had been previously reported.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 104 (1975), S. 23-28 
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Yeast ; Chemostat ; Nutrient Concentration ; Thermal Death ; Thermal Association ; Optimum Temperature for Growth ; Maximum Temperature for Growth ; Microbial Ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Saccharomyces cerevisiae was grown in a chemostat under glucose limitation at three superoptimal temperatures. In each steady state the specific growth rate was the sum of the dilution rate and the specific death rate, exponential death concurring with exponential growth. The specific death rate was a function of the temperature while the specific growth rate was a function of both the temperature and the concentration of the limiting nutrient. Each superoptimal temperature was characterized by a critical glucose concentration below which net growth was not possible. The critical glucose concentration increased with the temperature. Consequently the maximum temperature for growth was a function of the concentration of the limiting nutrient and approached the optimum temperature for growth with decreasing glucose concentrations.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 106 (1975), S. 271-273 
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Baker's yeast ; Gluconeogenetic enzymes ; Chemostat ; Oxygen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. The effect of aeration on the key enzymes of gluconeogenesis was studied in baker's yeast (Saccharomyces cerevisiae) and in a nonrespiratory variant of S. cerevisiae grown under glucose limitation. 2. In baker's yeast phosphoenolpyruvate carboxykinase, hexosediphosphatase and isocitrate lyase were completely repressed under anaerobic conditions. Their repression could be partially reversed by using intense aeration. 3. In the nonrespiratory variant these enzymes were absent independently of aeration. 4. Pyruvate carboxylase of baker's yeast showed maximal activity under anaerobic conditions. In the nonrespiratory variant pyruvate carboxylase had low activity under both anaerobic and aerobic conditions.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 276 (1975), S. 201-204 
    ISSN: 1618-2650
    Keywords: Best. von Glykolaldehyd, Glycerinaldehyd, Hydroxyaldehyden ; Volumetrie ; Dimere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wurden die Möglichkeiten für die titrimetrische Bestimmung von dimerem Glykolaldehyd (Fp: 94–96° C) und dl-Glycerinaldehyd (Fp: 138–141° C) untersucht. Die systematischen Fehler der auf Grund verschiedener funktioneller Gruppen durchführbaren Messungen sind von der Stabilität ihrer cyclischen Halbacetale sowie von den ihrer Depolymerisation folgenden weiteren Gleichgewichten bestimmt. Das aus dem dl-Glycerinaldehyd gebildete Halbacetal besitzt höhere Stabilität als dasjenige aus dem Glykolaldehyd. Mit der Hydrogensulfit-Methode werden 95–96%, mit der Hydroxylamin-Methode dagegen 98–99% vom wahren Wert gefunden. In Wasser gelöst zersetzt sich der Glykolaldehyd verhältnismäßig rasch, und das Gleichgewicht kann durch beide Aldehydreaktionen quantitativ gegen die Monomerenform verschoben werden. Unsere Untersuchungen ergaben, daß die Hydroxylamin-Methode zur Bestimmung der in Form cyclischer Halbacetale kristallisierenden, dimeren Hydroxyaldehyde Vorteile gegenüber der Hydrogensulfit-Methode bietet.
    Notes: Abstract Possibilities for the titrimetric determination of dimeric glycolaldehyde (m.p. 94–96° C) and dl-glyceraldehyde (m.p. 138–141° C) have been investigated. The systematic errors based on different functional groups depend on the stability of the cyclic semiacetals and on the subsequent equilibria established after their depolimerisation. The semiacetal of dl-glyceraldehyde is more stable than that of glycolaldehyde. 95–96% and 98–99% of the real content were found by the hydrogensulphite and hydroxylamine methods, respectively. Glycolaldehyde decomposes rather quickly in aqueous solution and the equilibrium can be shifted towards the monomeric form by both the aldehyde reactions. The hydroxylamine method proved to be more advantageous for the determination of the dimeric hydroxyaldehydes, which cristallise in the form of cyclic semiacetals.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 277 (1975), S. 125-125 
    ISSN: 1618-2650
    Keywords: Sulfonamiden mit N-Bromsuccinimid ; Volumetrie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 277 (1975), S. 207-207 
    ISSN: 1618-2650
    Keywords: Best. von Fluorid in Wasser ; Volumetrie ; SPADNS und Thoriumnitrat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 273 (1975), S. 109-111 
    ISSN: 1618-2650
    Keywords: Best. von Kohlenstoff, Wasserstoff in organ. Substanzen ; Volumetrie ; Proben von 30–300 μg
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The carbon- and hydrogen determination in 0.2–2 mg of organic sample, in which an automated titrimetric finish and a conversion of water into carbon dioxide was employed, was made suitable for samples of 30–300 μg. No radical changes in the apparatus are necessary. The time of an analysis is about 15 min or less. The standard deviation calculated from 20 analyses of sucrose is for C and H 0.23% abs. and 0.17% abs., resp.
    Notes: Zusammenfassung Die Kohlenstoff- und Wasserstoffbestimmung in Proben von 0,2–2 mg, bei der die Verbrennungsprodukte Kohlendioxid und Wasser (nach Konversion in Kohlendioxid) titimetrisch bestimmt werden, wurde mit kleinen Abänderungen derselben Apparatur zur Bestimmung in Proben von 30–300 μg angepaßt. Die Analysendauer bleibt beschränkt auf 15 min oder weniger. Die Standardabweichung, errechnet aus 20 Analysen von Saccharose, beträgt ür C bzw. H 0,23% bzw. 0,17% abs.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 273 (1975), S. 288-288 
    ISSN: 1618-2650
    Keywords: Best. von Cäsium als Cs-Mg-Cyanoferrat(II) ; Volumetrie ; ÄDTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 274 (1975), S. 29-29 
    ISSN: 1618-2650
    Keywords: Best. von Hydrazin ; Volumetrie ; Oxidation mit KMnO4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 274 (1975), S. 30-30 
    ISSN: 1618-2650
    Keywords: Best. von Eisen in Marmor ; Volumetrie ; ÄDTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 274 (1975), S. 126-126 
    ISSN: 1618-2650
    Keywords: Best. von Mercaptanen, Xanthogenaten, Disulfiden, organ ; Volumetrie ; N-Bromsuccinimid als Reagens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 274 (1975), S. 304-304 
    ISSN: 1618-2650
    Keywords: Best. von Kobalt mit Tropolon ; Volumetrie ; ÄDTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 274 (1975), S. 383-383 
    ISSN: 1618-2650
    Keywords: Best. von Xanthaten, Dithiocarbamaten ; Volumetrie ; oxidimetr., wasserunlösl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 275 (1975), S. 204-204 
    ISSN: 1618-2650
    Keywords: Best. von Nitrit ; Volumetrie ; Oxidation mit Dichromat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 275 (1975), S. 275-279 
    ISSN: 1618-2650
    Keywords: Bindung von Alkalien durch ÄDTA ; Volumetrie ; dilatometrischer Endpunkt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Mit Hilfe dilatometrischer Titrationen wird der komplexe Einbau von Alkaliionen in das ÄDTA-Chelatsystem verfolgt. Die Auswertung der Titrationskurven erlaubt einerseits die Angabe der pK-Werte für die einzelnen Komplexe, andererseits die Berechnung des Hydrathüllenvolumens der Alkaliionen. Außerdem kann gezeigt werden, daß Li- und Na-Ionen, sofern sie sich im Überschuß befinden, mit ÄDTA Ionenpaare bilden.
    Notes: Abstract The incorporation of alkali ions into the EDTA chelate system is followed by the dilatometric titration method. Evaluation of the titration curves permits, on one hand, the determination of the pK values for the individual complexes and, on the other hand, it allows the calculation of the volume of hydration sphere. Moreover, it can be shown that Li and Na ions, so long they are present in excess, form ion pairs with EDTA.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 275 (1975), S. 287-287 
    ISSN: 1618-2650
    Keywords: Best. von Thioharnstoffen, Mercaptanen mit Phenyljodosoacetat ; Volumetrie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 276 (1975), S. 189-191 
    ISSN: 1618-2650
    Keywords: Best. von Metallionen ; Volumetrie ; ÄDTA, alkalimetr. Methode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die in der Komplexometrie zur Bestimmung von einigen Metallionen angewandte alkalimetrische Titration, die auf der Bestimmung der bei der Bildung von ÄDTE-Metallkomplexen freiwerdenden H+ beruht, wurde unter Anwendung der Glaselektrode weiterentwickelt. Durch die Weiterentwicklung konnte eine bequemere Arbeitsweise mit einfacher Indikation bei großer Genauigkeit erzielt werden. Die Methode ist für ungepufferte Lösungen von Cd, Co, Cu, Mn(II), Pb und Zn anwendbar. Hg und Ca müssen in 50% äthanolischer Lösung titriert werden. Die relative Standardabweichung beträgt ca. ± 0,3%.
    Notes: Abstract The EDTA-titration for the determination of various metal ions by alkalimetry (based on the determination of H+ set free during the formation of EDTA-metal complexes) was further developed by using the glass electrode. With the improved method it was possible to make the determination more comfortable with a simple indication and a good accuracy. It is suitable for unbuffered solutions of Cd, Co, Cu, Mn(II), Pb and Zn. Hg and Ca must be titrated in 50% ethanolic solution. The relative standard deviation is approximately ± 0.3%.
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