ALBERT

Description: The International Cometary Explorer (ICE) encounter with Comet Giacobini-Zinner took place 7 years after the spacecraft's original launch on 12 August 1978 as the International Sun Earth Explorer 3 (ISEE-3), part of a three-spacecraft project to study the interaction between the solar wind and the Earth's magnetosphere. Transfer to an interplanetary trajectory was performed via a 119-km-altitute, gravity-assist, lunar swingby on December 1983. Navigation support during interplanetary cruise and comet encounter was provided by orbit determination utilizing radio metric data from the DSN 64-meter antennas in Goldstone, California and Madrid, Spain. Orbit solutions yielding predictions of 50-km geocentric delivery accuracy in the target aim plane were achieved during interplanetary cruise and at comet encounter using 6-to-12-week data arcs between periodic attitude-change maneuvers. One-sigma two-way range and range rate residuals were consistently 40 meters and 0.2 mm/s or better, respectively. Non-gravitational forces affected the comet's motion during late August and early September 1985 and caused a 2300-km shift in the orbit of the comet relative to the spacecraft. This necessitated a final ICE orbit trim maneuver 3 days prior to encounter. Near-real-time assessment of two-way 2-GHz (S-band) Doppler pseudo-residuals during the June and July 1985 trajectory change maneuvers aided in calibration of the spacecraft's thrusters in preparation for this final critical maneuver. Post-flight analysis indicates tail centerline passage was achieved within 10 seconds of the predicted time and geocentric position uncertainty at encounter was less than 40 km.

Description: This report describes the Mirror Transport Mechanism (MTM), which supports a pair of dihedral mirrors and moves them in a very smooth and uniform scanning motion normal to a beamsplitter. Each scan is followed by a quick flyback and repeat. Included in the report will be material selection, design, and testing of all major components of the MTM in order to meet the stringent performance requirements under cryogenic conditions and survive the launch environment of the shuttle. Areas to be discussed in detail will be those in which failures or performance anomalies occurred and their solutions. Typically, this will include (but not to be limited to) flex pivot failures during vibration testing, excessive dihedral platform sag under one "g" operation, electronic and fiber optic characteristics, and tolerancing considerations. As of this writing, development of the mechanism has reached the final phase of thermal and vibration qualification. Environmental testing of the complete FIRAS (Far Infrared Absolute Spectrophotometer) experiment is just beginning.

Description: The design of the Advanced Communications Technology Satellite MBA system, which provides both spot beam and scanning beam coverage to both high and low burst rates data-users is examined. The MBA consists of receive and transmit antennas installed on a common precision mounting platform that is integrated to the bus through three flexures; a lightweight system with low thermal distortion is obtained by using composite materials for the MBA structures. The RF design, which is a Cassegrain reflector with a large equivalent focal length/aperture size, is described. Consideration is given to the position of the feed in order to minimize scan loss and sidelobe levels, the size of the subreflector in order to minimize feed spillover, and antenna performance degradation caused by reflector surface distortion. Breadbroad model test result reveal that the maximum sidelobe level outside the 2.5 HPBW region is -30 dB or lower relative to the power.

Description: Adaptation to weightlessness includes the substitution of other sensory signals for the no longer appropriate graviceptor information concerning static spatial orientation. Visual-vestibular interaction producing roll circularvection was studied in weightlessness to assess the influence of otolith cues on spatial orientation. Preliminary results from four subjects tested on Spacelab-1 indicate that visual orientation effects were stronger in weightlessness than pre-flight. The rod and frame test of visual field dependence showed a weak post-flight increase in visual influence. Localized tactile cues applied to the feet in space reduced subjective vection strength.

Description: Tests of otolith function were performed pre-flight and post-flight on the science crew of the first Spacelab Mission with a rail-mounted linear acceleration sled. Four tests were performed using horizontal lateral (y-axis) acceleration: perception of linear motion, a closed loop nulling task, dynamic ocular torsion, and lateral eye deviations. The motion perception test measured the time to detect the onset and direction of near threshold accelerations. Post-flight measures of threshold and velocity constant obtained during the days immediately following the mission showed no consistent pattern of change among the four crewmen compared to their pre-flight baseline other than an increased variability of response. In the closed loop nulling task, crewmen controlled the motion of the sled and attempted to null a computer-generated random disturbance motion. When performed in the light, no difference in ability was noted between pre-flight and post-flight. In the dark, however, two of the four crewmen exhibited somewhat enhanced performance post-flight. Dynamic ocular torsion was measured in response to sinusoidal lateral acceleration which produces a gravitionertial stimulus equivalent to lateral head tilt without rotational movement of the head. Results available for two crewmen suggest a decreased amplitude of sinusoidal ocular torsion when measured on the day of landing (R+0) and an increasing amplitude when measured during the week following the mission.

Description: Reflex responses that depend on human otolith organ sensitivity were measured before, during and after a 10 day space flight. Otolith-spinal reflexes were elicited by means of sudden, unexpected falls. In weightlessness, "falls" were achieved using elastic cords running from a torso harness to the floor. Electromyographic (EMG) activity was recorded from gastrocnemius-soleus. The EMG response occurring in the first 100-120 ms of a fall, considered to be predominantly otolith-spinal in origin, decreased in amplitude immediately upon entering weightlessness, and continued to decline throughout the flight, especially during the first two mission days. The response returned to normal before the first post-flight testing session. The results suggest that information coming from the otolith organs is gradually ignored by the nervous system during prolonged space flight, although the possibility that otolith-spinal reflexes are decreased independent of other otolith output pathways cannot be ruled out.

Description: This paper reviews previous findings and introduces new material about otolith end organs that help us to understand their functioning and development. In particular, we consider the end organs as biological accelerometers. The otoconia are dealt with as test masses whose substructure and evolutionary trend toward calcite may prove significant in understanding formation requirements. Space-flight helps illuminate the influence of gravity, while right-left asymmetry is suggested by study of certain rat strains.

Description: Space sickness symptoms were observed by 4 specially trained observers on Spacelab-1. Three reported persistent symptoms, and vomited repeatedly during the first and/or second day of flight. Head movements on all axes were provocative, particularly in pitch and roll. Head acceleration data recorded from 2 symptomatic crewmen showed that after several hours of physical activity in orbit, symptoms appeared, and thereafter both crewmen were compelled to limit head movements. Firm body contact with motionless surfaces helped alleviate symptoms. When crewmembers floated into unfamiliar body orientations in the cabin, inherent ambiguities in static visual orientation cues sometimes produced spatial reorientation episodes which were also provocative. Symptoms largely resembled those of other forms of prolonged motion sickness, superimposed upon other symptoms attributable to fluid shift. All 4 eventually used anti-motion sickness drugs. When they did, vomiting frequency was reduced. By the 4th day, symptoms subsided, and head accelerations again increased in magnitude and variability. Sickness intensity in orbit was not predicted by statistically concordant results of 6 acute preflight susceptibility tests. However, results from a longer duration preflight prism goggles test showed an apparent correlation. All subjects were asymptomatic making head movements in parabolic flight 4 days after the mission, but not 1 year later. Overall, results support the view that space sickness is a motion sickness.

Description: Dynamic posture testing was conducted on the science crew of the Spacelab-1 mission on a single axis linear motion platform. Tests took place in pre- and post-flight sessions lasting approximately 20 min each. The pre-flight tests were widely spaced over the several months prior to the mission while the post-flight tests were conducted over the first, second, fourth, and sixth days after landing. Two of the crew members were also tested on the day of landing. Consistent with previous postural testing conducted on flight crews, these crew members were able to complete simple postural tasks to an acceptable level even in the first few hours after landing. Our tests were designed to induce dynamic postural responses using a variety of stimuli and from these responses, evaluate subtle changes in the postural control system which had occurred over the duration of the flight. Periodic sampling post-flight allowed us to observe the time course of readaptation to terrestrial life. Our observations of hip and shoulder position, when subjected to careful analysis, indicated modification of the postural response from pre- to post-flight and that demonstrable adjustments in the dynamic control of their postural systems were taking place in the first few days after flight. For transient stimuli where the platform on which they were asked to stand quickly moved a few centimeters fore or aft then stopped, ballistic or open loop 'programs' would closely characterize the response. During these responses the desired target position was not always achieved and of equal importance not always properly corrected some 15 seconds after the platform ceased to move. The persistent observation was that the subjects had a much stronger dependence on visual stabilization post-flight than pre-flight. This was best illustrated by a slow or only partial recovery to an upward posture after a transient base-of-support movement with eyes open. Postural responses to persistent wideband pseudorandom base-of-support translation were modeled as time invarient linear systems arrived at by Kalman adaptive filter techniques. Derived model parameters such as damping factor and fundamental frequency of the closed loop system showed significant modification between pre- and post-flight. This phenomenon is best characterized by movement of the poles toward increasing stability. While pre-flight data tended to show shoulders and hips moving in phase with each other, post-flight data showed a more disjoint behavior.(ABSTRACT TRUNCATED AT 400 WORDS).

Description: No abstract available

Description: Preliminary attempts by our group at UCSF to assess fat content of vertebral marrow in the lumbar spine using relaxation time information demonstrated that the presence of trabecular bone affects relaxation times. The objective of this work was a thorough study of the effects of bone on NMR relaxation characteristics of surrounding liquids. Trabecular bone from autopsy specimens was ground up and sifted into a series of powders with graded densities ranging from 0.3 gm/cc to 0.8 gm/cc. Each powder was placed first in n-saline and then in cottonseed oil. With spectroscopy, spin-lattice relaxation times (T1) and effective spin-spin relaxation times (T2*) were measured for each liquid in each bone powder. As bone density and surface to volume ratio increased, T1 decreased faster for saline than for oil. T2* decreased significantly for both water and oil as the surface to volume ratio increased. It was concluded that effects of water on T1 could be explained by a surface interaction at the bone/liquid interface, which restricted rotational and translational motion of nearby molecules. The T1s of oil were not affected since oil molecules are nonpolar, do not participate in significant intermolecular hydrogen bonding, and therefore would not be expected to interact strongly with the bone surface. Effects on T2* could be explained by local magnetic field inhomogeneities created by discontinuous magnetic susceptibility near the bone surface. These preliminary results suggest that water in contact with trabecular bone in vivo will exhibit shortened relaxation times.

Description: The ease with which a particular DNA segment adopts the left-handed Z-conformation depends largely on the sequence and on the degree of negative supercoiling to which it is subjected. We describe a computer program (Z-hunt) that is designed to search long sequences of naturally occurring DNA and retrieve those nucleotide combinations of up to 24 bp in length which show a strong propensity for Z-DNA formation. Incorporated into Z-hunt is a statistical mechanical model based on empirically determined energetic parameters for the B to Z transition accumulated to date. The Z-forming potential of a sequence is assessed by ranking its behavior as a function of negative superhelicity relative to the behavior of similar sized randomly generated nucleotide sequences assembled from over 80,000 combinations. The program makes it possible to compare directly the Z-forming potential of sequences with different base compositions and different sequence lengths. Using Z-hunt, we have analyzed the DNA sequences of the bacteriophage phi X174, plasmid pBR322, the animal virus SV40 and the replicative form of the eukaryotic adenovirus-2. The results are compared with those previously obtained by others from experiments designed to locate Z-DNA forming regions in these sequences using probes which show specificity for the left-handed DNA conformation.

Description: Exposure to conditions of weightlessness has been associated with decrements in muscle mass and strength. The present studies were undertaken to determine muscle responses at the cellular level. Male Sprague-Dawley rats (360-410 g) were exposed to 7 days of weightlessness during the Spacelab-3 shuttle flight (May 1985). Animals were killed 12 h postflight, and soleus (S), gastrocnemius (G), and extensor digitorum longus (EDL) muscles were excised. Muscle protein, RNA, and DNA were extracted and quantified. Differential muscle atrophy was accompanied by a significant (P less than 0.05) reduction in total protein only in S muscles. There were no significant changes in protein concentration (mg/g) in the muscles examined. In S muscles from flight animals, sarcoplasmic protein accounted for a significantly greater proportion of total protein that in ground controls (37.5 vs. 32.5%). Tissue concentrations (nmol/g) of asparagine-aspartate, glutamine-glutamate, glycine, histidine, and lysine were significantly reduced (from 17 to 63%) in S muscles from flight animals, but only glutamine-glutamate levels were decreased in the G and EDL. Muscle DNA content (microgram) was unchanged in the tissues examined, but S muscle DNA concentration (micrograms/mg) increased 27%. RNA content (micrograms) was significantly (P less than 0.025) reduced in S (-28%) and G(-22%) muscles following spaceflight. These results identify specific alterations in rat skeletal muscle during short term (7-day) exposure to weightlessness and compare favorably with observations previously obtained from ground-based suspension simulations.

Description: Despite impressive advances in aircraft technology over the past several decades and an overall decline in the airline accident rate since the introduction of turbine-powered aircraft, flight crew performance problems continue to dominate air transport accident statistics. Researchers have offered many hypotheses to explain this finding, and interest in pilot fatigue has stimulated a large volume of laboratory research. Much of this work, however, is difficult to generalize and to apply to the real world of flight operations, and researchers disagree about the extent and operational significance of fatigue-related reductions in pilot performance. As a result, in 1980 Congress asked the National Aeronautics and Space Administration to undertake a comprehensive research program to assess whether fatigue-related problems are prevalent in long- and short-haul flying. The two major goals of this project are: (1) to assess the psychophysiological effects on pilot performance of flying various types of flight and duty cycles, and (2) to determine the operational significance to flight safety and efficiency of flying these flight and duty cycles.

Description: When gibberellic acid (GA3) is sprayed on 9-day-old light-brown dwarf Progress pea (Pisum sativum) seedlings, arginine decarboxylase (ADC; EC 4.1.1.9) activity increases within 3 h and peaks at about 9 h after GA3 application. This is followed by a second lower peak at about 30 h; both peaks were higher than the corresponding peaks in the controls. In contrast, no appreciable effect of GA3 on internode length was observed until about 12 h, after which time a dramatic increase in growth rate occurred and persisted for about 12 h. Specific (DL-alpha-difluoromethylarginine) and non-specific (D-arginine and L-canavanine) inhibitors of ADC strongly inhibited ADC activity and to a lesser extent internode growth. The inhibition was reversed only slightly by the addition of polyamines. Actinomycin D and cycloheximide inhibited the rise in ADC activity induced by GA3. The half-life of the enzyme was increased by GA3 treatment. The results suggest that part of the GA3-induced increase in internode growth may result from enhanced polyamine biosynthesis through the ADC pathway. Furthermore, the GA3 induced increase in ADC activity probably requires de novo synthesis of both RNA and protein.

Description: Amyloplast sedimentation during gravistimulation and organelle movements was studied in living central rootcap cells of Zea mays L. cv. Merit. Cells from sectioned roots were viewed with a horizontally-mounted videomicroscope. The kinetics of gravity-induced amyloplast sedimentation were comparable to those calculated from experiments using fixed material. Individual amyloplasts fell at an average velocity of 5.5 micrometers min-1; the maximal velocity of fall measured was 18.0 micrometers min-1. Amyloplasts often rotated, sometimes rose in the cytoplasm, and occasionally underwent sudden rapid movements as fast as 58 micrometers min-1. Saltations of other organelles were frequently observed. This appears to be the first report of cytoplasmic streaming in the presumptive statocytes of roots.

Description: Roots of Allium cepa L. grown in aerated water elongate rapidly, but are not graviresponsive. These roots (1) possess extensive columella tissues comprised of cells containing numerous sedimented amyloplasts, (2) lack mucilage on their tips, and (3) are characterized by a weakly polar movement of calcium (Ca) across their tips. Placing roots in humid air correlates positively with the (1) onset of gravicurvature, (2) appearance of mucilage on tips of the roots, and (3) onset of the ability to transport Ca polarly to the lower side of the root tip. Gravicurvature of roots previously submerged in aerated water is more rapid when roots are oriented vertically for 1-2 h in humid air prior to being oriented horizontally. The more rapid gravicurvature of these roots correlates positively with the accumulation of mucilage at the tips of roots during the time the roots are oriented vertically. Therefore, the onset of gravicurvature and the ability of roots to transport Ca to the lower sides of their tips correlate positively with the presence of mucilage at their tips. These results suggest that mucilage may be important for the transport of Ca across root caps.

Description: This report includes procedures for ensuring the quality of the environment provided for plant growth in controlled environment facilities. Biologists and engineers may use these procedures for ensuring quality control during experiments or for ensuring quality control in the design of plant growth facilities. Environmental monitoring prior to and during experiments is included in these procedures. Specific recommendations cover control, acquisition, and calibration for sensor types for the separate parameters of radiation (light), temperature, humidity, carbon dioxide, and air movement.

Description: No abstract available

Description: A 50% increase in total radiation by extending the photoperiod from 16 to 24 hr doubled the weight of all cultivars of loose-leaf lettuce (Lactuca sativa L.) 'Grand Rapids Forcing', 'Waldmanns Green', 'Salad Bowl', and 'RubyConn', but not a Butterhead cultivar, 'Salina'. When total daily radiation (moles of photons) was the same, plants under continuous radiation weighed 30% to 50% more than plants under a 16 hr photoperiod. By using continuous radiation on loose-leaf lettuce, fewer lamp fixtures were required and yield was increased.

Description: Cereal leaves subjected to the osmotica routinely used for protoplast isolation show a rapid increase in arginine decarboxylase activity, a massive accumulation of putrescine, and slow conversion of putrescine to the higher polyamines, spermidine and spermine (HE Flores, AW Galston 1984 Plant Physiol 75: 102). Mesophyll protoplasts from these leaves, which have a high putrescine:polyamine ratio, do not undergo sustained division. By contrast, in Nicotiana, Capsicum, Datura, Trigonella, and Vigna, dicot genera that readily regenerate plants from mesophyll protoplasts, the response of leaves to osmotic stress is opposite to that in cereals. Putrescine titer as well as arginine and ornithine decarboxylase activities decline in these osmotically stressed dicot leaves, while spermidine and spermine titers increase. Thus, the putrescine:polyamine ratio in Vigna protoplasts, which divide readily, is 4-fold lower than in oat protoplasts, which divide poorly. We suggest that this differing response of polyamine metabolism to osmotic stress may account in part for the failure of cereal mesophyll protoplasts to develop readily in vitro.

Description: alpha-Difluoromethylornithine (DFMO), a specific and irreversible inhibitor of the polyamine biosynthetic enzyme ornithine decarboxylase, effectively inhibits mycelial growth of several phytopathogenic fungi on defined media in vitro and provides systemic protection of bean plants against infection by Uromyces phaseoli L. race 0 (MV Rajam, AW Galston 1985 Plant Cell Physiol 26: 683-692; MV Rajam et al. 1985 Proc Natl Acad Sci USA 82: 6874-6878). We now find that application of 0.5 millimolar DFMO to unifoliolate leaves of Pinto beans up to 3 days after inoculation with uredospores of U. phaseoli completely inhibits the growth of the pathogen, while application 4 or 5 days after inoculation results in partial protection against the pathogen. Spores do not germinate on the surface of unifoliolate leaves treated with DFMO 1 day before infection, but addition of spermidine to the DFMO treatments partially reverses the inhibitory effect. The titer of polyamines in bean plants did not decline after DFMO treatment; rather, putrescine and spermidine contents actually rose, probably due to the known but paradoxical stimulation of arginine decarboxylase activity by DFMO.

Description: The effects of Cd2+ on putrescine (Put), spermidine (Spd), and spermine (Spm) titers were studied in oat and bean leaves. Treatment with Cd2+ for up to 16 hours in the light or dark resulted in a large increase in Put titer, but had little or no effect on Spd or Spm. The activity of arginine decarboxylase (ADC) followed the pattern of Put accumulation, and experiments with alpha-difluoromethylarginine established that ADC was the enzyme responsible for Put increase. Concentrations of Cd2+ as low as 10 micromolar increased Put titer in oat segments. In bean leaves, there was a Cd(2+)-induced accumulation of Put in the free and soluble conjugated fractions, but not in the insoluble fraction. This suggests a rapid exchange between Put that exists in the free form and Put found in acid soluble conjugate forms. It is concluded that Cd2+ can act like certain other stresses (K+ and Mg2+ deficiency, excess NH4+, low pH, salinity, osmotic stress, wilting) to induce substantial increases in Put in plant cells.

Description: Columella (i.e., putative graviperceptive) cells of Zea mays seedlings grown in the microgravity of outer space allocate significantly less volume to putative statoliths (amyloplasts) than do columella cells of Earth-grown seedlings. Amyloplasts of flight-grown seedlings are significantly smaller than those of ground controls, as is the average volume of individual starch grains. Similarly, the relative volume of starch in amyloplasts in columella cells of flight-grown seedlings is significantly less than that of Earth-grown seedlings. Microgravity does not significantly alter the volume of columella cells, the average number of amyloplasts per columella cell, or the number of starch grains per amyloplast. These results are discussed relative to the influence of gravity on cellular and organellar structure.

Description: We have attempted to improve the viability of cereal mesophyll protoplasts by pretreatment of leaves with DL-alpha-difluoromethylarginine (DFMA), a specific 'suicide' inhibitor of the enzyme (arginine decarboxylase) responsible for their osmotically induced putrescine accumulation. Leaf pretreatment with DFMA before a 6 hour osmotic shock caused a 45% decrease of putrescine and a 2-fold increase of spermine titer. After 136 hours of osmotic stress, putrescine titer in DFMA-pretreated leaves increased by only 50%, but spermidine and spermine titers increased dramatically by 3.2- and 6-fold, respectively. These increases in higher polyamines could account for the reduced chlorophyll loss and enhanced ability of pretreated leaves to incorporate tritiated thymidine, uridine, and leucine into macromolecules. Pretreatment with DFMA significantly improved the overall viability of the protoplasts isolated from these leaves. The results support the view that the osmotically induced rise in putrescine and blockage of its conversion to higher polyamines may contribute to the lack of sustained cell division in cereal mesophyll protoplasts, although other undefined factors must also play a major role.

Description: The putrescine which forms a part of nicotine and other pyrrolidine alkaloids is generally assumed to arise through the action of ornithine decarboxylase (ODC). However, we have previously noted that changes in the activity of arginine decarboxylase (ADC), an alternate source of putrescine, parallel changes in tissue alkaloids, while changes in ODC activity do not. This led us to undertake experiments to permit discrimination between ADC and ODC as enzymatic sources of putrescine destined for alkaloids. Two kinds of evidence presented here support a major role for ADC in the generation of putrescine going into alkaloids: (a) A specific 'suicide inhibitor' of ADC effectively inhibits the biosynthesis of nicotine and nornicotine in tobacco callus, while the analogous inhibitor of ODC is less effective, and (b) the flow of 14C from uniformly labelled arginine into nicotine is much more efficient than that from ornithine.

Description: Graviperception by plant roots is believed to occur via the sedimentation of amyloplasts in columella cells of the root cap. This physical stimulus results in an accumulation of calcium on the lower side of the cap, which in turn induces gravicurvature. In this paper we present a model for root gravitropism integrating gravity-induced changes in electrical potential, cytochemical localization of calcium in cells of gravistimulated roots, and the interdependence of calcium and auxin movement. Key features of the model are that 1) gravity-induced redistribution of calcium is an early event in the transduction mechanism, and 2) apoplastic movement of calcium through the root-cap mucilage may be an important component of the pathway for calcium movement.

Description: The growing region of the upright Pharbitis nil shoot extends over a distance 13 cm basipetal to the shoot apex. When the shoot is inverted, ethylene production in this region is greatly enhanced whereas stem elongation is significantly inhibited. This growth region is ethylene-sensitive and the restriction of its growth by shoot inversion-induced ethylene may mediate the release of apical dominance.

Description: On July 7, 1984 pulsar 0656+14 was found to exhibit a larger period than expected. Using observations from a number of sources, we have attempted to explain this discrepancy. No indications of orbital motion was found. The available evidence indicates that the star has a period derivative of 54 10(-15) s s-1 rather than 1.6 10(-15) s s-1 as previously reported.

Description: With guidance from experiments and observations that indicate internally limited phenomena, an outline of unified evolutionary sequence is inferred. Such unification is not visible for a context of random matrix and random mutation. The sequence proceeds from Big Bang through prebiotic matter, protocells, through the evolving cell via molecular and natural selection, to mind, behavior, and society.

Description: A fiber-optic microphobe is described which is inexpensive and simple to build and use. It consists of an 80-micrometers optical fiber which at the end is tapered down to a rounded sensing tip of 20-30-micrometers diameter. The detector is a hybrid photodiode/amplifier. The probe has a sensitivity of 0.01 microEinst m-2 s-1 and a spectral range of 300-1,100 nm. Spectral light gradients were measured in fine-grained San Francisco Bay sediment that had an undisturbed diatom coating on the surface. The photic zone of the mud was only 0.4 mm deep. Measured in situ spectra showed extinction maxima at 430-520, 620-630, 670, and 825-850 nm due to absorption by chlorophyll a, carotenoids, phycocyanin, and bacterio-chlorophyll a. Maximum light penetration in the visible range was found in both the violet and the red 〈 or = 400 and 〉 or = 700 nm.

Description: The 1985 Antarctic Scientific Research Expedition to Lake Hoare in Taylor Valley is briefly described. Of particular interest to to the expedition is the nature of the lake's perennial ice cover and its role in concentrating dissolved gases. Also, the algal mats and sediment found on the bottom of the lake were studied. Antarctic lakes have been cited as possible analogs for possible biological habitats on Mars and on Europa.

Description: A method of analyzing 16S rRNA catalog data has been developed in which groupings at various taxonomic levels can be characterized in terms of specific "signature" oligonucleotides. This approach provides an alternative means for evaluating higher order branching possibilities and can be used to assess the phylogenetic position of isolates that are poorly placed by the usual clustering procedures. This signature approach has been applied to forty archaebacterial catalogs and every oligonucleotide with significant signature value has been identified. Sets of specific oligonucleotides were identified for every major group on a dendrogram produced by cluster analysis procedures. Signatures that would establish between group relationships were also sought and found. In the case of the Methanobacteriaceae the clustering methods suggest a specific relationship to the Methanococcaceae. This inclusion is in fact supported by six strong signature oligonucleotides. However there are also significant numbers of signature oligonucleotides supporting a specific relationship of the Methanobacteriaceae to either the Halobacteriaceae or the Methanomicrobiaceae. Thus the placement of the Methanobacteriaceae is less certain than the usual dendrograms imply. The signature approach also was used to assess the phylogenetic position of Thermoplasma acidophilum which is found to be more closely related to the methanogen/halophile Division than to the sulfur dependent Division of the archaebacteria. This does not imply however that Thermoplasma acidophilum is properly regarded as being in the methanogen/halophile Division.

Description: The evolutionary sequence is being reexamined experimentally from a "Big Bang"origin to the protocell and from the emergence of protocell and variety of species to Darwin's mental power (mind) and society (The Descent of Man). A most fundamentally revisionary consequence of experiments is an emphasis on endogenous ordering. This principle, seen vividly in ordered copolymerization of amino acids, has had new impact on the theory of Darwinian evolution and has been found to apply to the entire sequence. Herein, I will discuss some problems of dealing with teaching controversial subjects.

Description: Standard evolutionary theory sees little beyond the natural selection of random variation. Yet there is more to life.

Description: The vertical zonation of light, O2, H2S, pH, and sulfur bacteria was studied in two benthic cyanobacterial mats from hypersaline ponds at Guerrero Negro, Baja California, Mexico. The physical-chemical gradients were analyzed in the upper few mm at 〈 or = 100 micrometers spatial resolution by microelectrodes and by a fiber optic microprobe. In mats, where oxygen produced by photosynthesis diffused far below the depth of the photic zone, colorless sulfur bacteria (Beggiatoa sp.) were the dominant sulfide oxidizing organisms. In a mat, where the O2-H2S interface was close to the photic zone, but yet received no significant visible light, purple sulfur bacteria (Chromatium sp.) were the dominant sulfide oxidizers. Analysis of the spectral light distribution here showed that the penetration of only 1% of the incident near-IR light (800-900 nm) into the sulfide zone was sufficient for the mass development of Chromatium in a narrow band of 300 micromoles thickness. The balance between O2 and light penetration down into the sulfide zone thus determined in micro-scale which type of sulfur bacteria became dominant.

Description: The exercise response after bed rest inactivity is a reduction in the physical work capacity and is manifested by significant decreases in oxygen uptake. The magnitude of decrease in maximal oxygen intake V(dot)O2max is related to the duration of confinement and the pre-bed-rest level of aerobic fitness; these relationships are relatively independent of age and gender. The reduced exercise performance and V(dot)O2max following bed rest are associated with various physiological adaptations including reductions in blood volume, submaximal and maximal stroke volume, maximal cardiac output, sceletal muscle tone and strength, and aerobic enzyme capacities, as well as increases in venous compliance and submaximal and maximal heart rate. This reduction in physiological capacity can be partially restored by specific countermeasures that provide regular muscular activity or orhtostatic stress or both during the bed rest exposure. The understanding of these physiological and physical responses to exercise following bed rest inactivity has important implications for the solution to safety and health problems that arise in clinical medicine, aerospace medicine, sedentary living, and aging.

Description: A comparative isosteric ion adsorption study for minerals (kaolinite, rutile, and quartz) was performed in aqueous solutions of CaCl2, LaCl3, and Th(NO3)4 in the presence of the neutral salt NaCl. It was observed that the concentration of Ca(2+) ions required to produce a standard reduction in the electrophoretic mobility of mineral particles was always appreciably greater than the concentration required for the Th(4+) ions. The effectiveness of adsorption of the cations differed from particle to particle and showed that ion adsorption on a mineral surface depends, among other things, on the nature of the mineral surface and on the particular adsorbed cation. The number of cation binding sites on mineral surfaces and the electrochemical free energies of cation adsorption were calculated. It was found that the adsorption energy of La(3+) and Th(4+) ions on rutile, kaolinite, and quartz was greater than that of Ca(2+) on these minerals.

Description: Eight month old male C57BL6 mice were exposed without anesthesia to whole-body irradiation in circular holders. The mice were tested for behavioral decrements after 0.5 and 50 rads of Fe particle irradiation at 6 and 12 months post irradiation to obtain long term results. A standard maze was used and the animals were timed for completion thereof. A string test also was administered to the mice, testing their ability to grasp and move along a string to safety. The results from animals exposed to 50 rads were significantly different from [correction of fron] control results to p = 〈 .001 in both systems of testing. The hippocampus (believed to be the location of environmental interaction in the brain) and the retina were examined for ultrastructural changes. The ultrastructural changes were similar to those we found in our Cosmos 782, 936 and in our Argon experiments. The mouse data indicate that iron particles were able to induce long term changes in the central nervous system which lead to behavioral impairment.

Description: No abstract available

Description: No abstract available

Description: Recent findings related to the role of calcium ions in phytochrome responses are reviewed and summarized. Hypotheses tested are the activation of calmodulin by light-regulated Ca2+ transport in cells and the photoinduction of calmodulin-activated enzyme activities. Discussion focuses on evidence that Ca2+ helps to regulate phytochrome responses, calcium requirements for photoinduced spore germination in the fern Onoclea, Ca2+ fluxes and phytochrome function in the alga Mougeotia, calmodulin antagonist blocking of red-light stimulated chloroplast rotation, the role of phosphorylation in calmodulin-regulated responses, and phytochrome regulation of nuclear protein phosphorylation.

Description: The Challenger disaster focused attention on the hazards as well as the possibilities of man in space. The physiological effects of prolonged weightlessness include important changes in vestibular, bone, muscle, cardiovascular, blood, renal, and pulmonary function. Much has been learned from US and Soviet experiments, but large areas of ignorance remain. Exceptional opportunities for physiological research are provided by Spacelab, a pressurized laboratory planned as a payload of the Space Shuttle.

Description: Anyone undertaking an interstellar voyage might wish to be assured of the existence of a safe planetary harbor at the other end! Aside from the obvious interest of the participants in this Symposium, astronomers and astrophysicists are also eager to detect and study other planetary systems in order to better understand the formation of our own Solar System. Scientists involved in the search for extraterrestrial intelligence argue that planets suitable for the evolution of life may abound elsewhere within our own Milky Way Galaxy. On theoretical grounds, they are probably correct, but they lack any observational support. For in spite of decades of claimed astrometric detections of planetary companions and the recent exciting and tantalizing observations from the IRAS satellite and the IR speckle observations of Van Biesbroeck 8 and other cool stars, there is no unambiguous proof for the existence of another planetary system beyond our own. In this paper we review the various methods for detecting extrasolar planets and briefly describe the Earth and space based technology currently available and discuss the near-term plans to implement these different search techniques. In each case an attempt is made to identify the limiting source of systematic error inherent to the methodology and to assess the potential for technological improvements.

Description: Paracoccus halodenitrificans, grown anaerobically in the presence of nitrite, contained membrane and cytoplasmic nitrite reductases. When assayed in the presence of phenazine methosulfate and ascorbate, the membrane-bound enzyme produced nitrous oxide whereas the cytoplasmic enzyme produced nitric oxide. When both enzymes were assayed in the presence of methyl viologen and dithionite, the cytoplasmic enzyme produced ammonia. Following solubilization, the membrane-bound enzyme behaved like the cytoplasmic enzyme, producing nitric oxide in the presence of phenazine methosulfate and ascorbate, and ammonia when assayed in the presence of methyl viologen and dithionite. The cytoplasmic and membrane-bound enzymes were purified to essentially the same specific activity. Only a single nitrite-reductase activity was detected on electrophoretic gels and the electrophoretic behavior of both enzymes suggested they were identical. The spectral properties of both enzymes suggested they were cd-type cytochromes. These data suggest that the products of nitrite reduction by the cd-cytochrome nitrite reductase are determined by the location of the enzyme and the redox potential of the electron donor.

Description: This paper describes the design and development of a large diameter high pressure quick acting propulsion valve and valve actuator. The valve is the heart of a major test facility dedicated to conducting full scale performance tests of aircraft landing gear systems. The valve opens in less than 300 milliseconds releasing a 46 cm (18 in) diameter water jet and closes in 300 milliseconds. The four main components of the valve, i.e., valve body, safety shutter, high speed shutter, and pneumatic-hydraulic actuator, are discussed. This valve is unique and may have other aerospace and industrial applications.

Description: This paper will be divided into two parts. The first part will deal with the mechanical design of the keel latch. The second part will be the stress analysis of the keel latch. The first part will present: (1) background information; (2) mechanical design requirements; (3) some of the initial design considerations; (4) the design considerations that led to the selection of the final design; (5) the mechanics of the final design; (6) testing that has been and will be accomplished to verify that design requirements have been met; and (7) future tests.

Description: This paper describes the design requirements, the design, the assembly and alignment, and the test program for a shutter mechanism which must operate at cryogenic temperature and draw less than 1.0 milliwatt. The design solution to meet these requirements is a device that positions a mirror with repeated accuracy, has no wearing surfaces and operates at 2.0 K. The unique feature of this device is the simplicity of the mechanism, thus obtaining high reliability.

Description: The Capillary Pumped Loop (CPL) experiment, G-471 is a thermal control system with high density heat acquisition and transport capability. The CPL consists of two capillary pumped evaporators with integral heaters, a fluid loop charged with ammonia (NH3), a condenser plate (heat sink), and various control electronics. The purpose of the experiment is to demonstrate the capability of a capillary pumped system under zero gravity conditions for use in the thermal control of large scientific instruments, advanced orbiting spacecraft, and space station components.

Description: The aim of the experiment is to study solidification of different compositions of lead-tin. The weight of the material is quite high: 8 kilograms. Nearly 10% of the payload is sample weight. The dendritic growth and the effect of the absence of natural convection are of particular interest. The results from the flight processed samples will be compared with results from Earth processed samples in order to investigate the influence of the natural convection on the solidification process. The power systems, heat storage and rejection, and mechanical support are discussed in relationship to the scientific requirements.

Description: Selected hints derived from 5 years experience in experiment hardware design, hardware qualification, safety provisions, experiment testing, payload integration, flight preparation, battery transport, and postmission checkout are presented.

Description: The structural design of the Get Away Special (GAS) ejection system is described. Equipment specifications and modifications are illustrated. Future goals are listed.

Description: The main purpose of the Halogen Lamp Experiment (HALEX) was to investigate the operation of a halogen lamp during an extended period in a microgravity environment and to prove its behavior in space. The Mirror Heating Facilities for Crystal Growth and Material Science Experiments in space relies on one or two halogen lamps as a furnace to melt the specimens. The HALEX aim is to verify: full power operation of a halogen lamp for a period of about 60 hours; achievement of about 10% of its terrestrial life span; and operation of the halogen lamp under conditions similar to furnace operation.

Description: At the beginning of the Get-Away Special (GAS) flight program, the GAS Project flew a Flight Verification Payload on STS-3 to make measurements of the vibrations, acoustic and magnetic environments of the canister, and to obtain thermal profiles of internal and external components of the GAS system. Two types of GAS canister end caps (insulated end cap and silverized teflon covered end cap) were used on the two flights (STS-7 and STS-8) so that a direct comparison of the thermal performance was obtained. These observations were compared with a simple thermal model of the instrument in the GAS canister in order to assess whether such simple models can be useful to experimenters in predicting the thermal response of their payloads.

Description: Project MAUS is a part of the German material sciences program and provides autonomous payloads for the Space Shuttle. These payloads are housed in canisters which are identical with those of NASA's Get Away Special program. The main components of the hardware are: a standard system consisting of power supply, experiment control, data acquisition and the experiment modules containing experiment specific hardware. So far, three MAUS modules with experiments from the area of material sciences have been flown as GAS payloads. They deal with the effects of reduced sedimentation, convection phenomena caused by temperature gradients and the behavior of dispersed particles ahead of a solid/liquid interface.

Description: The results of the Get Away Special (GAS) Arts-Science payload G-38, processed in orbit on board the Space Shuttle Challenger during mission 41-G STS 17, October 5 to 13, l984 are explained. The payload G-38 was created as a unified Arts-Science payload that simultaneously explored the process of vapor deposition in the vacuum and weightlessness of the shuttle environment and created a series of space sculptures utilizing this process. The purpose of the experiment was to test the sputter deposition process in space and to create five subtle spherical sculptures with metallic coatings of gold, silver, platinum and chrome.

Description: Some of the design problems that are now being addressed in consideration of a microcontroller for the upcoming GAS payload are discussed. Microcontrollers will be used to run the experiments and to collect and store the data from those experiments. Some of the requirements for a microcontroller are to be small, lightweight, have low power consumption, and high reliability. Some of the solutions that were developed to meet these design requirements are discussed. At present, the experiment is still in the design stage and the final design may change from what is presented here. The search for new integrated circuits which will do all that is needed all in one package continues.

Description: Zeolites are hydrated, crystalline aluminosilicates with alkali and alkaling earth metals substituted into cation vacancies. Typically zeolite crystals are 3 to 8 microns. Larger cyrstals are desirable. Large zeolite crystals were produced (100 to 200 microns); however, they have taken restrictively long times to grow. It was proposed if the rate of nucleation or in some other way the number of nuclei can be lowered, fewer, larger crystals will be formed. The microgravity environment of space may provide an ideal condition to achieve rapid growth of large zeolite crystals. The objective of the project is to establish if large zeolite crystals can be formed rapidly in space.

Description: The current materials research being done in microgravity solidification and the future experimentation planned onboard a space shuttle mission is reported. The Department of Engineering Mechanics at the USAF Academy is developing a microgravity furnace to be used on board the space shuttle. The microgravity furnace will be used to conduct materials research dealing with such topics as immiscible alloy solidification. The purpose behind this research project is three-fold: to develop a simple, inexpensive, and easy to use furnace to conduct space materials research, to conduct a solidification experiment on a lead-zinc alloy in space that macrosegregates due to gravity, and to provide a research mechanism for students to get involved with space materials research.

Description: The Global Low Orbiting Message Relay (GLOMR) Satellite and its participation in the pioneering development of a Get Away Special (GAS) satellite launch capability is described. The GLOMR is a data relay communication satellite. Experiment objectives are to demonstrate the store-forward relay of data/messages and the communication to ground user equipment. The 150 pound brass satellite, powered by lead acid batteries and solar cells, includes processing, control and radio frequency electronics for data message handling and storage. The GLOMR is mounted through a Marman clamp ejection/retention pedestal to a five cubic foot GAS canister bottom plate. The GAS canister includes a Full Diameter Motorized Door Assembly lid which is opened remotely by astronauts for satellite launch.

Description: A sealed window assembly for the Get Away Special (GAS) payloads was developed from a synthetic fused silica. The details of the design and development of this window assembley are given.

Description: Thermocapillary flow and gaseous convection in microgravity were investigated in GAS payload G-0518 during Space Shuttle Mission 41-D. A cylinder of paraffin was supported and heated differentially from its ends to induce a melt from solid to liquid and drive thermocapillary flow in the resulting liquid phase. Laminar thermocapillary flow was observed in the liquid paraffin and found to show a transition to time-dependent oscillatory motion at a Marangoni number of about Ma = 34000 with a period of approximately T = 8 seconds. In addition, free convection in a gas in microgravity was observed for the first time. The gaseous convection was caused by the thermal and/or velocity boundary layers present at the heater-liquid interface. Oscillation occurred in the gaseous convection simultaneously with those in the liquid, implying the two are strongly coupled. The gaseous convection may be driven by coupled thermocapillary flow/thermal expansion convection or microgravity bouyancy convection.

Description: Four hours, fourteen minutes and forty-two seconds after the space shuttle, Challenger, was launched on mission 51B, a small satellite was ejected into independent orbit from a Getaway Special canister. This event was an exciting milestone in a project conceived over seven years ago. It is hoped that the story of NUSAT (Northern Utah SATellite) will be an inspiration for other experimentors to exploit this new service of the Getaway Special program. The purpose and history of the project, the NUSAT spacecraft, the ground station and its operation, and some future directions envisioned by the participants are described.

Description: The highlights of the Utah State University's participation in the space program are listed. Proposed experiments include: a study of the velocity of a bubble in water under the influence of a temperature gradient; reflight of an experiment on surface tension driven convective flow; surface waves in zero-G; crystallization in zero-G (vapor phase and liquid phase); bio gas generation; and penicillum growth; study of undamped oscillations in a vacuum and zero-G. The effect that spinoffs have had on the Utah State University were discussed.

Description: The Get Away Special (GAS) G-025, which flew on shuttle Mission 51-G, examined the behavior of a liquid in a tank under microgravity conditions. The experiment is representative of phenomena occurring in satellite tanks with liquid propellants. A reference fluid in a hemispherical model tank will be subjected to linear acceleration inputs of known levels and frequencies, and the dynamic response of the tank liquid system was recorded. Preliminary analysis of the flight data indicates that the experiment functioned perfectly. The results will validate and refine mathematical models describing the dynamic characteristics of tank-fluid systems. This will in turn support the development of future spacecraft tanks, in particular the design of propellant management devices for surface tension tanks.

Description: The Mitre Corporation of Bedford Mass. and the Worcester Polytechnic Institute are developing several experiments for a future Shuttle flight. Several design practices for the development of the electrical equipment for the flight hardware have been standardized. Some of the ideas are presented, not as hard and fast rules but rather in the interest of stimulating discussions for sharing such ideas.

Description: What happens if a stainless steel ball hits a water ball in the weightless space ot the Universe? In other words, it was the objective of our experiments in the Space to observe the surface tension of liquid by means of making a solid collide with a liquid. Place a small volume of water between 2 glass sheets to make a thin water membrane: the 2 glass sheets cannot be separated unless an enormous force is applied. It is obvious from this phenomenom that the surface tension of water is far greater than presumed. On Earth, however, it is impossible in most cases to observe only the surface tension of liquid, because gravity always acts on the surface tension. Water and stainless steel balls were chosen the liquid and solids for the experiments. Because water is the liquid most familiar to us, its properties are well known. And it is also of great interest to compare its properties on the Earth with those in the weightless space.

Description: This paper describes a space qualified, precision, large force, thermally activated driver that has been developed jointly by the NASA Langley Research Center and PRC Kentron. The driver consists of a sealed hydraulic cylinder containing a metal bellows, a bellows plug, a coil spring, a spring retainer, an output shaft, a shaft guide, and a quantity of silicone oil. Temperature changes cause the silicone oil to expand or contract thus contracting or expanding the bellows/spring assembly thereby extending or retracting the output shaft.

Description: The results are presented herein of a test program that evaluated the stiffness, accuracy, torque ripple, frictional losses, and torque holding capability of a 16:1 ratio, 430 N-m (320 ft-lb) planetary roller drive for a potential space vehicle actuator application. The drive's planet roller supporting structure and bearings were found to be the largest contributors to overall drive compliance, accounting for more than half of the total. In comparison, the traction roller contacts themselves contributed only nine percent of the drive's compliance based on an experimentally verified stiffness model. The drive exhibited no backlash although 8 arc sec of hysteresis deflection were recorded due to microcreep within the contact under torque load. Because of these load-dependent displacements, some form of feedback control would be required for arc second positioning applications. Torque ripple tests showed the drive to be extremely smooth, actually providing some damping of input torsional oscillations. The drive also demonstrated the ability to hold static torque with drifts of 7 arc sec or less over a 24 hr period at 35 percent of full load.

Description: A number of top-level considerations which affect Mars and vehicle selection are discussed. Indications are provided of the nature and severity of the impact of these considerations on missions and vehicles. Various types of missions, such as Mars fly-bys, Mars orbiting and landing mission, and missions to the moons of Mars are identified and discussed. Mission trajectories and opportunities are discused briefly. The different types of vehicles required in a Mars program are also discussed. Discussion includes several potential Earth-to-Orbit (ETO) vehicles, Mars surface vehicles, and 2 types of Orbit-to-Orbit (OTO) vehicles. Indications are provided as to preference for some of the concepts discussed.

Description: Final revisions were made in the study of continuum spectrophotometry of 17 comets done at Lick Observatory. Changes based upon this study were introduced into the Divine-Newburn cometary modelling paradigm. Work was begun on modifications to all of the theory based upon the direct nucleus and coma observations made by the Halley space missions.

Description: The optical properties of comet p/Halley are being investigated. Initial research has revealed the basic characteristics of the nucleus and provided unique insights into the formation and structure of the coma and the plasma tail. The study is among the most intensive conducted for any comet. The results from comet p/Halley may be useful in the interpretation of results from other comets.

Description: Over 20 asteroids were observed photometrically for period determinations and phase relation studies. These included 1036 Ganymed, the largest Mars-crossing asteroid known, and 1627 Ivar, an earth-approaching asteroid which is a potential space mission target. Doppler images of P/Halley from TMO were obtained. Initial testing of the JPL/TMO CCD camera system has supported by imaging P/Giacobini-Zinner and P/Halley.

Description: A change coupled device (CCD) is used in a scanning mode to find new asteroids and recover known asteroids and comet nuclei. Current scientific programs include recovery of asteroids and comet nuclei requested by the Minor Planet Center (MPC), discovery of new asteroids in the main belt and of unusual orbital types, and follow-up astrometry of selected new asteroids discovered. The routine six sigma limiting visual magnitude is 19.6 and slightly more than a square degree is scanned three times every 90 minutes of observing time during the fortnight centered on New Moon. Semiautomatic software for detection of moving objects is in routine use; angular speeds as low as 11.0 arcseconds per hour were distinguished from the effects of the Earth's atmosphere on the field of view. A typical set of three 29-minute scans near the opposition point along the ecliptic typically nets at least 5 new main-belt asteroids down to magnitude 19.6. In 18 observing runs (months) 43 asteroids were recovered, astrometric and photometric data on 59 new asteroids were reported, 10 new asteroids with orbital elements were consolidated, and photometry and positions of 22 comets were reported.

Description: The first asteroid lightcurves have now been made with a new technique of CCD photometry. The apparent magnitude is fainter (V〉17) than what can be done with the 1.52-m Catalina reflector with a photomultiplier photometer. With the CCD system, however, the lightcurve shows remarkably good repetition; finding the asteroid is, of course, no problem as the object is recognized later by its motion on the CCD. Asteroid 1985RV has a lightcurve amplitude of about 0.4 mag and its period of rotation P = 4.0 hours, on the assumption that the lightcurve has two maxima and two minima as is the case for nearly all other asteroids. The diameter is about 3 km. 1985RV is a first example of results that are being obtained on asteroids and comets with CCD in the Catalinas, Kitt Peak, and Cerro Tololo.

Description: Infrared spectroscopic studies of the solar system in support of spacecraft investigations were researched. The studies include the physical behavior of comets, the atmospheres of the gaseous planets, and the solid surfaces of satellites and asteroids. Studies of the gaseous and solid emanations of comets using the techniques of CCD spectroscopy and imaging were focused on. An intensive observational effort was mounted, to observe comet Halley with the recently developed state of the art CCD camera system. Comet P/Giacobini-Zinner was observed. The major emphasis was placed on spectroscopy and a synoptic spectrum at least once a month was obtained. A large number of images of Halley for particular emission species and continuum regions were also obtained. The species in vestigated were: C3 (4060 A), CO+ (4505), C2 (5139), NH2 (5980), H2O+ (6185), OI (6300) and CN (9180).

Description: A very extensive Io sodium imaging program beginning with the first images of the cloud and culminating in comprehensive characterization of the cloud's appearance and behavior as a function of both time and Io's orbital position now forms the basis for a collaborative modeling analysis and a continued observational study with emphasis on understanding how the cloud's behavior is diagnostic of spatial and temporal variations in the physical conditions of the inner Jovian magnetosphere. The sodium cloud was found to have a variety of systematic variations, a pronounced east-west orbital asymmetry, and temporal changes which could be diagnostic of time changes in both Io and its plasma environment. New comet imaging programs initiated at the 3.6-meter Canada-France-Hawaii Telescope draw upon both ultra high time resolution and high spatial resolution imaging capabilities. Seven observing runs between Dec. 1984 and May 1986 were devoted to observing Halley, Giacobini-Zinner, and several other comets. Among these observations were the first images of Halley through IHW filters, and images of Giacobini-Zinner during the ICE encounter which have been directly correlated with ICE magnetic field measurements.

Description: Diffraction-limited telescopic observations were obtained of solar system objects and a program of research into Uranus, Neptune, Pluto and the asteroids based upon the data obtained was persued. Two camera systems appropriate for this work were developed. Most importantly, the PAPA photon address camera was developed and proven. Algorithms were developed for both phase and amplitude recovery and were validated on theoretical and laboratory data and to a limited extent on telescopic data. A laboratory simulator was constructed that was used for development but is also available for controlled investigation of image reconstruction. During 1985 two successful expeditions were made to Hawaii and Cerro Tololo and a large body of data on Pluto, Uranus, Neptune and two asteroids are on tape.

Description: Spectroscopic and spectrophotometric data on the atmospheres of comets, the outer planets, and Titan at ultraviolet to near-infrared wavelengths were acquired. These data support an effort aimed at characterizing the physical properties and distribution of aerosol particles in the atmospheres of these bodies. New spectrophotometry was acquired of Uranus at 0.2 lambda 0.3 micrometer with IUE; archival IUE spectrophotometry of Uranus and Neptune in this wavelength range was acquired and recalibrated. New estimates of radiometric Bond albedos and global energy budges of Uranus, Neptune and Titan were published.

Description: 511 independent fields were photographed with the 0.46m Schmidt during 15 observing runs along with a limited number of 1.2m Schmidt exposures. From these fields 122 new asteroids were reported. Special sets of field using the 1.2m Schmidt are continuing to yield numerous new faint asteroids. The combined result from both telescopes include 4 Mars-crossers, 3 Hungarias, 2 Phocaeas, a unique high inclination object (1985XB), 1 Apollo (1985PA) and Aten (3362) Khufu. Halley was regularly monitored (astrometry and large-scale phenomena) IHW participation. Scientifically value photographs were obtained from both 0.46m and 1.2m Schmidt Telescopes. Other accessible comets were observed including G-Z. Recently a new comet (1986d) was discovered.

Description: Speckle interferometry is a high angular resolution technique that allows study of resolved asteroids. By following the changing size, shape, and orientation of minor planets, and with a few general assumptions (e.g., geometric scattering, triaxial ellipsoid figures, no albedo features), it is possible to directly measure an asteroid's true dimensions and the direction of its spin axis in one or two nights. A particular subset of triaxial ellipsoid figures are equilibrium shapes, and would imply that some asteroids are thoroughly fractured. Such shapes if they exist among the asteroids would allow a determination of bulk density since there is a unique relation among spin period, size, shape, and density. The discovery of even a single rubble pile, (just as the finding of even one binary asteroid by speckle interferometric techniques) would drastically alter the notion of asteroids as small solid planets. The Pluto/Charon system was studied to aid in improving the orbital elements necessary to predict the eclipse/occultation season currently in progress. Four asteroids were reduced to their size, shape, and pole direction: 433 Eros, 532 Herculina, 511 Davida, and 2 Pallas.

Description: Infrared images and photometry were obtained to determine the spatial distribution and physical characteristics (temperature, albedo, size distribution, total mass, etc.) of the grains in the coma of Comet GZ. A 10.8 m image of Comet GZ obtained on August 4 represents the first groundbased thermal-infrared image of a Comet. Among the most significant results are: (1)An estimate of the number of grains that the ICE spacecraft must have encountered, which led the plasma wave team to conclude that they could only detect impacts on the antennae and not on the whole body of the ICE spacecraft; (2)The discovery of a population of large grains (radius 〉 100 micrometer), not observed in most other comets, which formed a curved tail near the nucleus (within 80 arcsec or 34,000 km); and (3)the detection of structure in the spatial distribution in the coma of the particle albedo, which was tentatively attributed to the presence of very fluffy grains which are likely to have multiple internal scattering of incident sunlight. The albedo map of Comet GZ was obtained by combining the 10.8 micrometer image shown with a simultaneous image taken at 0.68 micrometer, a bandpass which isolates the scattered continuum.

Description: An Atlas of Comet Halley 1910 II photographs and spectra is being prepared. The major section consists of 838 photographic observations from fifteen observatories around the world. Multiple images of many photographs are reproduced to bring out detail in the near nucleus region, in the coma and in the tail. The Atlas contains a total of 1209 photographic images of the 1910 apparition. In addition there are sections showing drawings from 1935 and 1910. A short section compares 1910 drawings and photographs. The final two sections display digitally processed images from 1910 and 1910 spectra. A three part appendix contains diagrams of various data associated with the 1910 apparition, a set of tables of all 1910 images and a bibliography.

Description: The first comprehensive spectrophotometric survey in the near-infrared (0.8 to 2.5 microns) was carried out with the NASA Infrared Telescope Facility and special filters optimized for spectroscopy of solid surfaces. About 105 asteroids have been observed (in 52 photometric passbands) to date. These spectra provide a much improved database for determining the mineralogy of the various asteroid spectral classes previously defined on the basis of visible (0.3 to 1.0 micron) data. Preliminary results from analysis of these spectra include: (1)Class "A" asteroids are dominated by olivine and correspond to the rare brachinite meteorites; (2)Class "S" asteroids have highly variable olivine/pyroxene ratios inconsistent with chondritic compositions; (3)Classes "P" and "D" are apparently "ultracarbonacous" material which extends the known range of chondrites to lower condensation temperatures; (4)The distribution of spectral types shows that the asteroids were heated by a mechanism whose intensity declined rapidly with solar distance; and (5)Spectra of the proposed flyby targets for CRAF were used to evaluate the results obtainable from this mission.

Description: Catalogues of approx. 200 decametric arcs and approx. 200 gaps between arcs were studied, in an effort to reconcile the data with predictions for the model wherein reflections of Io-induced currents each emit in a conical pattern and yield a distinct radio arc. The most recent interpretations of this data suggest that these Io-produced Alfven waves persist for at least one or two passages of Io, and that the emission cone half angles are approx. 40 to 90 deg., varying from arc to arc. Below 1.2 MHz it was discovered that Jupiter emits radiation strongly modulated in frequency with periods of approx. 200 kHz; this quasi-sinusoidal emission (MSA) can shift more than 180 deg. in phase over periods of 6 seconds, although these shifts are usually much smaller. MSA is not strongly correlated with the longitudes of Io or Jupiter, and typically occurs in patches covering approx. 500 kHz or more for periods of a few minutes. Furthermore, this modulation sometimes resembles a train of impulses in frequency with exponential decays toward high frequencies. Comparison of these results with the previous studies of V-shaped S-bursts is suggestive of an emission mechanism.

Description: Infrared data was obtained on planetary atmospheres which provides critical information on atmospheric structure, composition, and cloud properties in support of planetary missions such as Voyager and Galileo. Mapping of Jupiter and Saturn in thermal and reflected solar radiation is a high-priority monitoring and exploratory activity. Some of these images of Jupiter are shown. Radiation at 17.8 micrometer probes the upper tropospheric temperature structure where spatial structure bears a strong resemblance to visible and near-infrared reflected sunlight such as at 2.0 micrometer. At 7.8 micrometer, stratospheric temperatures appear to have a three-banded structure, enhancements near the magnetic poles and occasional transient features such as the equatorial filament near the right limb. Clouds or hazes are observed high in the stratosphere looking at wavelengths such as 2.2 micrometer, where gaseous opacity is very strong. Other maps examine cloud properties from thermal radiation not strongly influenced by gaseous opacity and the distribution of condensable gases, such as ammonia. Millimeter and submillimeter filtered radiometric observations were made of Jupiter, Uranus and Neptune via collaborative work. Radiometric observations of Uranus and Neptune at 21 and 32 micrometer were acquired and analyzed as well as grating array spectra in the ranges of 8 to 14 micrometer, 16 to 23 micrometer, and 18 to 32 micrometer. These showed evidence for C2H2 in the stratosphere of Uranus and C2H4 in the stratosphere of Neptune.

Description: Infrared observations were made of the outer planet satellites. These data provide vital information about the thermophysical properties of satellite surfaces, including internal heat sources for Io. Observations include both broad and narrow band measurements in the 2 to 20 micrometer spectral range. Types of observation and target priority were determined to make maximum use of existing data from Voyager and other missions, on-going and planned missions such as Galileo, were supported and techniques and data for planning new missions and instrumentation were developed.

Description: The general system configuration for the Mark 4A Deep Space Network is described. The arrangement and complement of antennas at the communications complexes and subsystem equipment at the signal processing centers are described. A description of the Network Operations Control Center is also presented.

Description: A low-loss bandstop filter designed and developed for the Deep Space Network's 34-meter high-efficiency antennas is described. The filter is used for protection of the X-band traveling wave masers from the 20-kW transmitter signal. A combination of empirical and theoretical techniques was employed as well as computer simulation to verify the design before fabrication.

Description: A system for the functional testing and simulation of custom and semicustom very large scale integrated (VLSI) chips that are designed using the integrated UNIX-based computer-aided design (CAD) system is described. The testing and simulation system consists of two parts. One of these is a special purpose hardware device that is capable of controlling the digital imputs and outputs on a custom chip. This device, the Digital Microcircuit Functionality Tester (DMFT) system, can be operated by itself or in conjunction with the VAX host computer on the CAD system. The DMFT is integrated into a microprobe station so that these signals can be injected or read from nodes inside the chip, as well as at the pins. The second part of the system is a software package that is installed on the VAX. This software package, logic, includes a full-screen editor for developing chip test sequences and drivers for both the DMFT and the esim logic simulator.

Description: Telemetry signals received from the Voyager 2 spacecraft at Deep Space Stations at Parkes and Canberra, Australia, on February 6, 1986, were combined by the method of symbol stream combining. This second demonstration of symbol stream combining followed the International Cometary Explorer (ICE) demonstration at Giacobini-Zinner encounter in September 1985. The Voyager demonstration was at a symbol rate of 43.2 ksymb/s, compared to 2 ksymb/s for ICE. Recording, playback, and combining at this higher rate were demonstrated. The average symbol signal-to-noise ratio (SNR) of the combined data was 2.84 dB, or 0.23 dB less than the sum of the SNRs of the two imput symbol streams. This 0.23 loss from ideal combining was due to use of 4-bit quantization of the input symbol stream and imperfect scaling. A practical implementation with 8-bit quantization could achieve combining losses of under 0.05 dB over a wide dynamic range of input signal levels.

Description: Previous work in the area of choosing channel quantization levels for a additive white Gaussian noise channel composed of one receiver-demodulator is reviewed, and how this applies to the Deep Space Network composed of several receiver-demodulators (space diversity reception) is shown. Viterbi decoding for the resulting quantized channel is discussed.

Description: Field tests of SETI (Search for Extraterrestrial Intelligence) prototype hardware and software began in March 1985 at Goldstone. With emphasis on the sky survey component of the NASA SETI search strategy, the article describes the survey characteristics, the detection strategy, and preliminary results of system tests.

Description: As part of the Network Consolidation Program, the 26-meter Tracking and Communication Subnet was transferred to JPL. Along with this transfer JPL assumed responsibility for tracking and navigation support for Earth orbiter missions normally tracked by the 26-meter sites. The High Earth Orbiter (HEO) Multimission Navigation Facility was formed as a component of the Deep Space Network (DSN) Tracking System for the purpose of supporting Earth orbiter missions and certain classes of deep space missions. This facility has been implemented on a dedicated VAX 11/780 minicomputer within the Network Operations Control Center (NOCC). The primary function of the system is to process radio metric data and estimate the orbit of a spacecraft in near-Earth or deep space environment. The system is capable of processing radio metric data in near-real time and providing the quick turnaround required for Earth orbiter operations. It is also capable of generating precision spacecraft ephemeris for use by the NOCC Support Subsystem and external agencies. This article discusses the implementation and functional operation of the Multimission Navigation Subsystem and describes the support that has been provided for an array of missions.

Description: Results of station frequency stability testing performed at DSS 13 (Deep Space Station) during May 1985 through March 1986 are presented. The testing was done on X-band uplink and X- and S-band downlink subsystems as well as on end-to-end systems. The subsystem test data are useful for assessing the frequency stability of various prototype X-band uplink or downlink subsystems for purposes of making design improvements. Information derived from extensive testing at DSS 13 will be useful in the preparation of an X-band Uplink Demonstration Experiment to be conducted at DSS 13, and will also be valuable in the preparations of gravity wave experiments to be conducted at other DSN stations in the future.