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  • Articles  (62,751)
  • Articles: DFG German National Licenses  (62,751)
  • Chemistry  (62,298)
  • General Chemistry  (13,154)
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  • GEOPHYSICS
  • LUNAR AND PLANETARY EXPLORATION
  • Saccharomyces cerevisiae
  • 1995-1999  (38,906)
  • 1965-1969  (23,845)
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  • Articles  (62,751)
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  • 1
    Electronic Resource
    Electronic Resource
    Identification of a fungal triacetylfusarinine C siderophore transport gene (TAF1) in Saccharomyces cerevisiae as a member of the major facilitator superfamily (1999)
    Springer
    BioMetals 12 (1999), S. 301-306 
    Details
    ISSN: 1572-8773
    Keywords: iron ; siderophores ; transport ; Saccharomyces cerevisiae ; fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Transport proteins of microorganisms may either belong to the ATP-binding cassette (ABC) superfamily or to the major facilitator (MFS)-superfamily. MFS transporters are single-polypeptide membrane transporters that transport small molecules via uniport, symport or antiport mechanisms in response to a chemiosmotic gradient. Although Saccharomyces cerevisiae is a non-siderophore producer, various bacterial and fungal siderophores can be utilized as an iron source. From yeast genome sequencing data six genes of the unknown major facilitator (UMF) family were known of which YEL065w Sce was recently identified as a transporter for the bacterial siderophore ferrioxamine B (Sit1p). The present investigation shows that another UMF gene, YHL047c Sce, encodes a transporter for the fungal siderophore triacetylfusarinine C. The gene YHL047c Sce (designated TAF1) was disrupted using the kanMX disruption module in a fet3 background (strain DEY 1394 Δfet3), possessing a defect in the high affinity ferrous iron transport. Growth promotion assays and transport experiments with 55Fe-labelled triacetylfusarinine C showed a complete loss of iron utilization and uptake in the disrupted strain, indicating that TAF1 is the gene for the fungal triacetylfusarinine transport in Saccharomyces cerevisiae and possibly in other siderophore producing fungi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009252118050
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  • 2
    Electronic Resource
    Electronic Resource
    Interaction of Saccharomyces cerevisiae with gold: toxicity and accumulation (1999)
    Springer
    BioMetals 12 (1999), S. 289-294 
    Details
    ISSN: 1572-8773
    Keywords: accumulation ; gold ; proton efflux ; Saccharomyces cerevisiae ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract This paper examines the effects of ionic gold on Saccharomyces cerevisiae, as determined by long-term (growth in gold-containing media) and short-term interactions (H+ efflux activity). An increasing gold concentration inhibited growth and at 〈0.2 mM Au, growth was not observed. Transmission electron microscopy revealed no differences in ultrastructure but fine electron dense particles were observed in unstained preparations from gold-containing medium. After glucose addition (to 10mM) to starved suspensions of S. cerevisiae, glucose-dependent reduction of external pH occurred as the cells extruded protons. In the presence of increasing gold concentrations, the lag time before proton extrusion did not change but the rate and duration decreased significantly with a marked influence on proton efflux rate being observed at ≤ 10 μM. Extension of preincubation time of yeast cells in gold-containing medium resulted in a decreasing proton efflux rate and colloidal phase formation in the cell suspensions, the time between gold addition and the beginning of colloidal phase formation depending on the gold concentration used. Both Ca and Mg enhanced the inhibitory effect of gold on the yeast cells with Ca showing a stronger inhibitory effect than Mg.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009210101628
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of otolith chemistry in Nassau grouper (Epinephelus striatus) from the Bahamas and Belize using solution-based ICP-MS (1999)
    Springer
    Coral reefs 18 (1999), S. 171-178 
    Details
    ISSN: 1432-0975
    Keywords: Key words Otolith ; Chemistry ; ICP-MS ; Stock discrimination ; Epinephelus striatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract  We examined the utility of otolith minor and trace element chemistry, assayed with inductively coupled plasma mass spectrometry (ICP-MS), as a means of delineating population structure in the Nassau grouper (Epinephelus striatus). We characterized the elemental composition of otoliths collected in 1993 from three locations in Exuma Sound, Bahamas and from Glover Reef, Belize in 1995. A single location in Exuma Sound was sampled in 1994 to test temporal variability in otolith composition. Five elements (Ca, Zn, Sr, Ba and Pb) were routinely detected, at levels significantly above background, by solution-based ICP-MS. Results from analysis of variance of elemental data, expressed as a ratio to Ca, indicated that there were no significant differences among the Exuma locations for any element, but significant variability was found between Glover Reef and the pooled Exuma localities for Zn/Ca, Sr/Ca and Ba/Ca ratios. Significant inter-annual differences at one Exuma Sound location was restricted to Ba/Ca ratios. Discriminant function analysis correctly classified 86% and 95% of the Belize and pooled Exuma sites, respectively. Otoliths from Belize were characterized by low Zn/Ca and high Ba/Ca and Pb/Ca ratios compared to otoliths from fish collected in Exuma Sound. Although differences in Ba levels may be related to upwelling at Glover Reef, more data are needed to definitely link otolith composition with regional differences in water chemistry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003380050176
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  • 4
    Electronic Resource
    Electronic Resource
    Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases (1999)
    Springer
    Current genetics 35 (1999), S. 609-617 
    Details
    ISSN: 1432-0983
    Keywords: Key words Cysteine uptake ; Amino-acid permeases ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uptake by Saccharomyces cerevisiae of the sulphur-containing amino acid L-cysteine was found to be non-saturable under various conditions, and uptake kinetics suggested the existence of two or more transport systems in addition to the general amino-acid permease, Gap1p. Overexpression studies identified BAP2, BAP3, AGP1 and GNP1 as genes encoding transporters of cysteine. Uptake studies with disruption mutants confirmed this, and identified two additional genes for transporters of cysteine, TAT1 and TAT2, both very homologous to BAP2, BAP3, AGP1 and GNP1. While Gap1p and Agp1p appear to be the main cysteine transporters on the non-repressing nitrogen source proline, Bap2p, Bap3p, Tat1p, Tat2p, Agp1p and Gnp1p are all important for cysteine uptake on ammonium-based medium. Furthermore, whereas Bap2p, Bap3p, Tat1p and Tat2p seem most important under amino acid-rich conditions, Agp1p contributes significantly when only ammonium is present, and Gnp1p only contributes under the latter condition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050459
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  • 5
    Electronic Resource
    Electronic Resource
    Starvation in yeast increases non-adaptive mutation (1999)
    Springer
    Current genetics 35 (1999), S. 77-81 
    Details
    ISSN: 1432-0983
    Keywords: Key words Adaptive mutations ; 6-N-hydroxylaminopurine ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The frequency of reversion in a histidine-requiring mutant of Saccharomyces cerevisiae increases about ten-fold in stationary cells during histidine starvation. Histidine starvation enhances a similar frequency of reversion in a tryptophan-requiring mutant. Starvation, therefore, enhances mutation frequencies in a non-adaptive manner. The base analogue 6-N-hydroxylaminopurine (HAP) added prior to plating on medium with limited histidine strongly increases reversion of the histidine mutant. HAP-induced reversion increases further in stationary starving cells with the same kinetics as that which increases spontaneous reversion. Adding HAP to the stationary starving cells does not produce any effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050435
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  • 6
    Electronic Resource
    Electronic Resource
    Strand interruptions confer strand preference during intracellular correction of a plasmid-borne mismatch in Saccharomyces cerevisiae (1999)
    Springer
    Current genetics 35 (1999), S. 499-505 
    Details
    ISSN: 1432-0983
    Keywords: Key words Heteroduplex repair ; Strand discrimina-tion ; Strand interruptions ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Site-directed mutagenesis was used to construct yeast centromere plasmids in which a strand nick or gap could be placed 5′ or 3′, on either strand, to a reporter gene (SUP4-o) carrying defined base mismatches. The plasmids were then transformed into yeast cells and the direction and efficiency of mismatch repair were assayed by scoring colouring of the transformant colonies. Strands that were nicked were consistently corrected more often than intact strands, but the effect was very small. However, placement of a small gap at the same positions as the nicks resulted in a marked increase in selection for the gapped strand and an enhanced efficiency of mismatch repair. Both the preference for the gapped strand and correction of the mismatch were offset by deletion of the mismatch repair gene PMS1. Together, the results suggest that strand interruptions can direct intracellular mismatch correction of plasmid-borne base mispairs in yeast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050445
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of the genes activated by the FLO8 gene in Saccharomyces cerevisiae (1999)
    Springer
    Current genetics 36 (1999), S. 256-261 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsFLO8 ; Transcriptional regulation ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It is thought that the FLO8 gene encodes a transcriptional activator of the dominant flocculation gene FLO1 in Saccharomycescerevisiae. To determine other genes which are regulated by FLO8, a detailed comparison of the transcripts from the FLO8 and Δflo8 strains was carried out. In addition to the FLO1 gene, it was found that transcription of the FLO11 and STA1 genes is positively regulated by FLO8. In flo8 strains, not only transcripts of the FLO11, STA1, and FLO1 genes but also invasive growth, extracellular glucoamylase production, and flocculation were undetected. From these results, it is suggested that FLO8 regulates these characteristics via the transcriptional regulation of the FLO11, STA1, and FLO1 genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050498
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  • 8
    Electronic Resource
    Electronic Resource
    Mutant allele pso7-1, that sensitizes Saccharomyces cerevisiae to photoactivated psoralen, is allelic with COX11, encoding a protein indispensable for a functional cytochrome c oxidase (1999)
    Springer
    Current genetics 36 (1999), S. 124-129 
    Details
    ISSN: 1432-0983
    Keywords: Key words Psoralen sensitivity ; Cytochrome oxidase ; Saccharomyces cerevisiae ; Oxidative stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The yeast gene PSO7 was cloned from a genomic library by complementation of the pso7-1 mutant's sensitivity phenotype to 4-nitroquinoline-1-oxide (4NQO). Sequence analysis revealed that PSO7 is allelic to the 1.1-kb ORF of the yeast gene COX11 which is located on chromosome XVI and encodes a protein of 28-kDa localized in the inner mitochondrial membrane. Allelism of PSO7/COX11 was verified by non-complementation of 4NQO-sensitivity in diploids homo- and hetero-allelic for the pso7-1 and cox11::TRP1 mutant alleles. Sensitivity to 4NQO was the same in exponentially growing cells of the pso7-1 mutant and the cox11::TRP1 disruptant. Allelism of COX11 and PSO7 indicates that the pso7 mutant's sensitivity to photoactivated 3-carbethoxypsoralen and to 4NQO is not caused by defective DNA repair, but rather is due to an altered metabolism of the pro-mutagen 4NQO in the absence of cytochrome oxidase (Cox) in pso7-1/cox11::TRP1 mutants/disruptants. Lack of Cox might also lead to a higher reactivity of the active oxygen species produced by photoactivated 3-carbethoxypsoralen. The metabolic state of the cells is important for their sensitivity phenotype since the largest enhancement of sensitivity to 4NQO between wild-type (WT) and the pso7 mutant occurs in exponentially growing cells, while cells in stationary phase or growing cells in phosphate buffer have the same 4NQO resistance, irrespective of their WT/mutant status. Strains containing the pso7-1 or cox11::TRP1 mutant allele were also sensitive to the oxidative stress-generating agents H2O2 and paraquat. Mutant pso7-1, as well as disruptant cox11::TRP1, harboured mitochondria that in comparison to WT contained less than 5% and no detectable Cox activity, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050481
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  • 9
    Electronic Resource
    Electronic Resource
    Comparative effects of Saccharomyces cerevisiae cultivation under copper stress on the activity and kinetic parameters of plasma-membrane-bound H+-ATPases PMA1 and PMA2 (1999)
    Springer
    Archives of microbiology 171 (1999), S. 273-278 
    Details
    ISSN: 1432-072X
    Keywords: Key words Plasma membrane H+-ATPase ; PMA1 ; ATPase ; PMA2 ATPase ; Saccharomyces cerevisiae ; Copper stress ; Copper tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The major yeast plasma membrane H+-ATPase is encoded by the essential PMA 1 gene. The PMA 2 gene encodes an H+-ATPase that is functionally interchangeable with the one encoded by PMA 1 , but it is expressed at a much lower level than the PMA 1 gene and it is not essential. Using genetically manipulated strains of Saccharomyces cerevisiae that exclusively synthesize PMA1 ATPase or PMA2 ATPase under control of the PMA1 promoter, we found that yeast cultivation under mild copper stress leads to a similar activation of PMA2 and PMA1 isoforms. At high inhibitory copper concentrations (close to the maximum that allowed growth), ATPase activity was reduced from maximal levels; this decrease in activity was less important for PMA2 ATPase than for PMA1 ATPase. The higher tolerance to high copper stress of the artificial strain synthesizing PMA2 ATPase exclusively, as compared to that synthesizing solely PMA1 ATPase, correlated both with the lower sensitivity of PMA2 ATPase to the deleterious effects of copper in vivo and with its higher apparent affinity for MgATP, and suggests that plasma membrane H+-ATPase activity plays a role in yeast tolerance to copper.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050710
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  • 10
    Electronic Resource
    Electronic Resource
    A strange calmodulin of yeast (1999)
    Springer
    Molecular and cellular biochemistry 190 (1999), S. 47-54 
    Details
    ISSN: 1573-4919
    Keywords: calmodulin ; yeast calmodulin ; Ca2+ binding ; Ca2+ binding protein ; Saccharomyces cerevisiae ; interdomain interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Calmodulin of Saccharomyces cerevisiae has different Ca2+ binding properties from other calmodulins. We previously reported that the maximum number of Ca2+ binding was 3 mol/mol and the fourth binding site was defective, which was different from 4 mol/mol for others. Their macroscopic dissociation constants suggested the cooperative three Ca2+ bindings rather than a pair of cooperative two Ca2+ bindings of ordinary calmodulin. Here we present evidence for yeast calmodulin showing the intramolecular close interaction between the N-terminal half domain and the C-terminal half domain, while the two domains of ordinary calmodulin are independent of each other. We will discuss the relationship of the shape and the shape change caused by the Ca2+ binding to the enzyme activation in yeast. The functional feature of calmodulin in yeast will also be considered, which might be different from the one of vertebrate calmodulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006951710440
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  • 11
    Electronic Resource
    Electronic Resource
    Characterization of Saccharomyces cerevisiae strains expressing ira1 mutant alleles modeled after disease-causing mutations in NF1 (1999)
    Springer
    Molecular and cellular biochemistry 202 (1999), S. 109-118 
    Details
    ISSN: 1573-4919
    Keywords: NF1 mutations ; IRA1 ; Saccharomyces cerevisiae ; RAS2 ; GAP activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The 2818 amino acids of neurofibromin, the product of the human NF1 gene, include a 230 amino acid Ras-GAP related domain (GRD). Functions which may be associated with the rest of the protein remain unknown. However, many NF1 mutations in neurofibromatosis 1 patients are found downstream of the GRD, suggesting that the C-terminal region of the protein is also functionally important. Since the C-terminal region of neurofibromin encompassing these mutations is homologous with the corresponding regions in the two Saccharomyces cerevisiae Ras-GAPs, Ira1p and Ira2p, we chose yeast as a model system for functional exploration of this region (Ira-C region). Three missense mutations that affect the Ira-C region of NF1 were used as a model for the mutagenesis of IRA1. The yeast phenotypes of heat shock sensitivity, iodine staining, sporulation efficiency, pseudohyphae formation, and GAP activity were scored. Even though none of the mutations directly affected the Ira1p-GRD, mutations at two of the three sites resulted in a decrease in the GAP activity present in ira1 cells. The third mutation appeared to disassociate the phenotypes of sporulation ability and GAP activity. This and other evidence suggest an effector function for Ira1p.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007058427880
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  • 12
    Electronic Resource
    Electronic Resource
    Direct observation of oxidative stress on the cell wall of Saccharomyces cerevisiae strains with atomic force microscopy (1999)
    Springer
    Molecular and cellular biochemistry 201 (1999), S. 17-24 
    Details
    ISSN: 1573-4919
    Keywords: Saccharomyces cerevisiae ; atomic force microscope ; bioscope ; organic synthesis ; molecular biology ; oxidative stress ; pore enlargement ; cell wall ; baker's yeast ; biotechnology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We imaged pores on the surface of the cell wall of three different industrial strains of Saccharomyces cerevisiae using atomic force microscopy. The pores could be enlarged using 10 mM diamide, an SH residue oxidant that attacks surface proteins. We found that two strains showed signs of oxidative damage via changes in density and diameter of the surface pores. We found that the German strain was resistant to diamide induced oxidative damage, even when the concentration of the oxidant was increased to 50 mM. The normal pore size found on the cell walls of American strains had diameters of about 200nm. Under conditions of oxidative stress the diameters changed to 400nm. This method may prove to be a useful rapid screening process (45-60 min) to determine which strains are oxidative resistant, as well as being able to screen for groups of yeast that are sensitive to oxidative stress. This rapid screening tool may have direct applications in molecular biology (transference of the genes to inside of living cells) and biotechnology (biotransformations reactions to produce chiral synthons in organic chemistry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007007704657
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  • 13
    Electronic Resource
    Electronic Resource
    Characterization of the Oxaloacetate Decarboxylase and Pyruvate Kinase-like Activities of Saccharomyces cerevisiae and Anaerobiospirillum succiniciproducens Phosphoenolpyruvate Carboxykinases (1999)
    Springer
    The protein journal 18 (1999), S. 659-664 
    Details
    ISSN: 1573-4943
    Keywords: Phosphoenolpyruvate carboxykinase ; oxaloacetate decarboxylase ; pyruvate kinase-like activity ; Anaerobiospirillum succiniciproducens ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Two members of the ATP-dependent class of phosphoenolpyruvate carboxykinases (PEPCKs) (Saccharomyces cerevisiae and Anaerobiospirillum succiniciproducens) have been comparatively studied with regard to their oxaloacetate (OAA) decarboxylase and pyruvate kinase-like activities. The pyruvate kinase-like activities were dependent on the presence of Mn2+; at the same concentrations Mg2+ was not effective. These activities were synergistically activated by a combination of both metal ions. V max for these activities in A. succiniciproducens and S. cerevisiae PEPCKs was 0.13% and 1.2% that of the principal reaction, respectively. The OAA decarboxylase activity was nucleotide independent and, with decreasing order of effectiveness, these activities were supported by Mn2+ and Mg2+. AMP is an activator of these reactions. V max for the OAA decarboxylase activities in A. succiniciproducens and S. cerevisiae PEPCKs was 4% and 0.2% that of the PEP-forming reaction, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020602222808
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  • 14
    Electronic Resource
    Electronic Resource
    Genetic evidence for interaction between components of the yeast 26S proteasome: combination of a mutation in RPN12 (a lid component gene) with mutations in RPT1 (an ATPase gene) causes synthetic lethality (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 145-153 
    Details
    ISSN: 1617-4623
    Keywords: Key words Proteasome ; Synthetic lethality ; Saccharomyces cerevisiae ; AAA-ATPase ; 19S Regulatory particle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 19S regulatory particle of the yeast 26S proteasome consists of six related ATPases (Rpt proteins) and at least 11 non-ATPase proteins (Rpn proteins). RPN12 (formerly NIN1) encodes an Rpn component of the 19S regulatory particle and is essential for growth. To determine which subunit(s) of the 26S proteasome interact(s) with Rpn12, we attempted to screen for mutations that cause synthetic lethality in the presence of the rpn12-1 (formerly nin1-1) mutation. Among the candidates recovered was a new allele of RPT1 (formerly CIM5). This mutant allele was designated rpt1-2; on its own this mutation caused no phenotypic change, whereas the rpn12-1 rpt1-2 double mutant was lethal, suggesting a strong interaction between Rpn12 and Rpt1. The site of the rpt1-2 mutation was determined by DNA sequencing of the RPT1 locus retrieved from the mutant, and a single nucleotide alteration was found. This changes amino acid 446 of the RPT1 product from alanine to valine. The alanine residue is conserved in all Rpt proteins, except Rpt5, but no function has yet been assigned to the region that contains it. We propose that this region is necessary for Rpt1 to interact with Rpn12. The terminal phenotype of the rpn12-1 rpt1-2 double mutant was not cell cycle specific, suggesting that in the double mutant cells the function of the 26S proteasome is completely eliminated, thereby inducing multiple defects in cellular functions.
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    URL: http://dx.doi.org/10.1007/s004380051069
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  • 15
    Electronic Resource
    Electronic Resource
    Cat8p, the activator of gluconeogenic genes in Saccharomyces cerevisiae, regulates carbon source-dependent expression of NADP-dependent cytosolic isocitrate dehydrogenase (Idp2p) and lactate permease (Jen1p) (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 869-875 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsCAT8 ; Transcriptional regulation ; IDP2 ; JEN1 ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The yeast transcriptional activator Cat8p has been identified as a factor that is essential for the derepression of genes involved in gluconeogenesis (like FBP1, PCK1, ACR1, ICL1 and MLS1) when only non-fermentable carbon sources are provided. Cat8p-dependent expression is mediated by cis-acting elements in the respective promoters, which are named UAS/CSREs (upstream activating sequence/carbon source responsive element). To establish whether the function of Cat8p is restricted to the activation of gluconeogenesis or is also involved in the regulation of a greater variety of genes, we investigated the transcriptional regulation of two genes, IDP2 and JEN1, which exhibit a similar expression pattern to gluconeogenic genes, although IDP2 at least is not linked directly to the gluconeogenic pathway. We identified functional UAS/CSRE elements in the promoters of both genes. Expression studies revealed that JEN1 is regulated negatively by the repressors Mig1p and Mig2p, and that Cat8p is needed for full derepression of the gene under non-fermentative growth conditions. Furthermore, we showed that Mig2p is also involved in the repression of CAT8 itself. The results presented in this study support a model in which Cat8p-dependent gene activation is not restricted to gluconeogenesis, but targets a wide variety of genes which are strongly derepressed under non-fermentative growth conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051152
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  • 16
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    Electronic Resource
    Yeast genes GIS1–4: multicopy suppressors of the Gal− phenotype of snf1 mig1 srb8/10/11 cells (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 589-599 
    Details
    ISSN: 1617-4623
    Keywords: Key words Ras/cAMP pathway ; Saccharomyces cerevisiae ; Snf1 ; Mig1 ; Mediator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyclin C and the cyclin C-dependent protein kinase are associated with the RNA polymerase II Mediator complex, which regulates initiation of transcription in response to signals from activators and repressors bound to upstream promoter elements. Disruption of the corresponding genes, SRB11 and SRB10, in budding yeast causes a reduction in expression of the GAL genes, which is particularly pronounced in a mig1 snf1 background. We have screened two yeast genomic libraries for genes that can suppress this phenotype when overexpressed. Seven suppressor genes were identified, GIS1–7. GIS1 encodes one of two related zinc-finger proteins, which also share two other highly conserved domains present in several eukaryotic transcription factors. GIS2 encodes a homologue of the mammalian CNBP and fission yeast Byr3 proteins. GIS3 and GIS4 predict proteins with no obvious similarities to any known proteins. GIS5–7 are identical to the previously described genes PDE2, SGE1 and TUB3, respectively. None of the suppressor genes seem to be involved in Mediator function. Instead, we find that the GIS1, GIS2 and GIS4 genes interact with the CDC25 gene, indicating a possible involvement of these genes in the RAS/cAMP signaling pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051121
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  • 17
    Electronic Resource
    Electronic Resource
    Genetic evidence for interactions between yeast importin α (Srp1p) and its nuclear export receptor, Cse1p (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 788-795 
    Details
    ISSN: 1617-4623
    Keywords: Key words Cse1p ; Srp1p ; Importin ; Nuclear transport ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The yeast Srp1p protein functions as an import receptor for proteins bearing basic nuclear localization signals. Cse1p, the yeast homolog of mammalian CAS, recycles Srp1p back to the cytoplasm after import substrates have been released into the nucleoplasm. In this report we describe genetic interactions between SRP1 and CSE1. Results from genetic suppression and synthetic lethality studies demonstrate that these gene products interact to ensure accurate chromosome segregation. We also describe new mutant alleles of CSE1 and analyze a new temperature-sensitive allele of CSE1, cse1-2. This allele causes high levels of chromosome missegregation and cell cycle arrest during mitosis at the nonpermissive temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050022
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  • 18
    Electronic Resource
    Electronic Resource
    The Saccharomyces cerevisiae LEP1/SAC3 gene is associated with leucine transport (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 332-341 
    Details
    ISSN: 1617-4623
    Keywords: Key words Leucine transport ; Saccharomyces cerevisiae ; Trifluoroleucine resistance ; LEP1 ; SAC3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Leucine uptake by Saccharomyces cerevisiae is mediated by three transport systems, the general amino acid transport system (GAP), encoded by GAP1, and two group-specific systems (S1 and S2), which also transport isoleucine and valine. A new mutant defective in both group-specific transport activities was isolated by employing a gap1 leu4 strain and selecting for trifluoroleucine-resistant mutants which also showed greatly reduced ability to utilize l-leucine as sole nitrogen source and very low levels of [14C]l-leucine uptake. A multicopy plasmid containing a DNA fragment which complemented the leucine transport defect was isolated by selecting for transformants that grew normally on minimal medium containing leucine as nitrogen source and subsequently assaying [14C]l-leucine uptake. Transformation of one such mutant, lep1, restored sensitivity to trifluoroleucine. The complementing gene, designated LEP1, was subcloned and sequenced. The LEP1 ORF encodes a large protein that lacks characteristics of a transporter or permease (i.e., lacks hydrophobic domains necessary for membrane association). Instead, Lep1p is a very basic protein (pI of 9.2) that contains a putative bipartite signal sequence for targeting to the nucleus, suggesting that it might be a DNA-binding protein. A database search revealed that LEP1 encodes a polypeptide that is identical to Sac3p except for an N-terminal truncation. The original identification of SAC3 was based on the isolation of a mutant allele, sac3-1, that suppresses the temperature-sensitive growth defect of an actin mutant containing the allele act1-1. Sac3p has been previously shown to be localized in the nucleus. When a lep1 mutant was crossed with a sac3 deletion mutant, no complementation was observed, indicating that the two mutations are functionally allelic.
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    URL: http://dx.doi.org/10.1007/s004380051091
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    Galactose induction in yeast involves association of Gal80p with Gal1p or Gal3p (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 495-507 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsKluyveromyces lactis ; Saccharomyces cerevisiae ; GAL1 ; GAL80 ; Protein interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gal1p carries out two functions in the galactose pathway of yeast. It activates Gal4p by interacting with Gal80p – a function that can also served by Gal3p – and it catalyzes the formation of galactose-1-phosphate. Recently, we and others have presented biochemical evidence for complex formation between Gal1p and Gal80p. Here, we extend these data and present genetic evidence for an interaction between Gal1p and Gal80p in vivo, using a two-hybrid assay. Interaction between Gal1p and Gal80p depends on the presence of galactose, but not on the catalytic activity of Gal1p. A new class of Kluyveromyces lactis mutants was isolated, designated Klgal1-m, which have lost the derepressing activity but retain galactokinase activity, indicating that the two Gal1p activities are functionally independent. The KlGal1-m proteins are defective in their ability to interact with Gal80p in a two-hybrid assay. The locations of gal1-m mutations identify putative interaction sites in Gal1p and Gal80p. A dominant mutation, KlGAL1-d, leads to a high level of constitutive expression of genes of the galactose pathway. The behavior of chimeric proteins consisting of Gal3p and KlGal1p sequences indicates that both the N-terminal and C-terminal halves of KlGal1p are involved in specific interaction with KlGal80p.
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    URL: http://dx.doi.org/10.1007/s004380050993
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    The mitochondrial cytochrome c peroxidase Ccp1 of Saccharomyces cerevisiae is involved in conveying an oxidative stress signal to the transcription factor Pos9 (Skn7) (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 437-447 
    Details
    ISSN: 1617-4623
    Keywords: Key words Oxidative stress signalling ; Mitochondria ; Pos9 (Skn7) ; Ccp1 ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Saccharomyces cerevisiae two transcription factors, Pos9 (Skn7) and Yap1, are involved in the response to oxidative stress. Fusion of the Pos9 response-regulator domain to the Gal4 DNA-binding domain results in a transcription factor which renders the expression of a GAL1-lacZ reporter gene dependent on oxidative stress. To identify genes which are involved in the oxygen-dependent activation of the Gal4-Pos9 hybrid protein we screened for mutants that failed to induce the heterologous test system upon oxidative stress (fap mutants for factors activating Pos9). We isolated several respiration-deficient and some respiration-competent mutants by this means. We selected for further characterization only those mutants which also displayed an oxidative-stress-sensitive phenotype. One of the respiration-deficient mutants (complementation group fap6) could be complemented by the ISM1 gene, which encodes mitochondrial isoleucyl tRNA synthetase, suggesting that respiration competence was important for signalling of oxidative stress. In accordance with this notion a rho0 strain and a wild-type strain in which respiration had been blocked (by treatment with antimycin A or with cyanide) also failed to activate Gal4-Pos9 upon imposition of oxidative stress. Another mutant, fap24, which was respiration-competent, could be complemented by CCP1, which encodes the mitochondrial cytochrome c peroxidase. Mitochondrial cytochrome c peroxidase degrades reactive oxygen species within the mitochondria. This suggested a possible sensor function for the enzyme in the oxidative stress response. To test this we used the previously described point mutant ccp1 W191F , which is characterized by a 104-fold decrease in electron flux between cytochrome c and cytochrome c peroxidase. The Ccp1W191F mutant was still capable of activating the Pos9 transcriptional activation domain, suggesting that the signalling function of Ccp1 is independent of electron flux rates.
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    URL: http://dx.doi.org/10.1007/s004380051103
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    A human gene, hSGT1, can substitute for GCR2, which encodes a general regulatory factor of glycolytic gene expression in Saccharomyces cerevisiae (1999)
    Springer
    Molecular genetics and genomics 260 (1999), S. 535-540 
    Details
    ISSN: 1617-4623
    Keywords: Key words Gene expression ; Glycolysis ; GCR ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To determine whether similar regulatory mechanisms control the expression of glycolytic genes in yeast and human cells, we screened a human brain cDNA library for clones which complement the growth defect of the gcr2 mutant of Saccharomyces cerevisiae, and isolated hSGT1 (human suppressor of GCR two). Further work confirmed that the rescue of growth was associated with recovery of glycolytic enzyme activities, and that hSGT1 did not complement the growth defect of a gcr1 mutant. A hybrid protein comprising hSgt1p and the DNA-binding domain of Gal4p (GBD) activated a GAL1-lacZ reporter gene fusion, suggesting that the cloned gene may be a transcriptional activator. Two-hybrid experiments in yeast also indicate that hSgt1p interacts with Gcr1p. Northern analysis showed that hSGT1 is highly expressed in muscle and heart. Although the predicted amino acid sequence of hSgt1p does not display significant similarity to Gcr2p, we speculate that their functions may be analogous.
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    URL: http://dx.doi.org/10.1007/s004380050926
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    The Saccharomyces cerevisiae RAD54 gene is important but not essential for natural homothallic mating-type switching (1999)
    Springer
    Molecular genetics and genomics 260 (1999), S. 551-558 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsRAD54 ; Saccharomyces cerevisiae ; Recombination ; Mating-type ; DNA repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Homothallic Saccharomyces cerevisiae strains switch their mating-type in a specific gene conversion event induced by a DNA double strand break made by the HO endonuclease. The RAD52 group genes control recombinational repair of DNA double strand breaks, and we examined their role in native homothallic mating-type switching. Surprisingly, we found that the Rad54 protein was important but not essential for mating-type switching under natural conditions. As an upper limit, we estimate that 29% of the rad54 spore clones can successfully switch their mating-type. The RAD55 and RAD57 gene products were even less important, but their presence increased the efficiency of the process. In contrast, the RAD51 and RAD52 genes are essential for homothallic mating-type switching. We propose that mating-type switching in RAD54 mutants occurs stochastically with a low probability, possibly reflecting different states of chromosomal structure.
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    URL: http://dx.doi.org/10.1007/s004380050928
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    Heterologous expression in Saccharomyces cerevisiae of an Arabidopsis thaliana cDNA encoding mevalonate diphosphate decarboxylase (1999)
    Springer
    Plant molecular biology 39 (1999), S. 953-967 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; heterologous expression ; isoprenoids ; mevalonate diphosphate decarboxylase ; sterols ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequence comparison with the mevalonate diphosphate decarboxylase (MVD) amino acid sequence of Saccharomyces cerevisiae identified an EST clone corresponding to a cDNA that may encode Arabidopsis thaliana MVD (AtMVD1). This enzyme catalyses the synthesis of isopentenyl diphosphate, the building block of sterol and isoprenoid biosynthesis, and uses mevalonate diphosphate as a substrate. Sequencing of the full-length cDNA was performed. The predicted amino acid sequence presents about 55% identity with the yeast, human and rat MVDs. The sequence of the genomic region of A. thaliana MVD was also obtained and Southern blot analysis on genomic DNA showed that A. thaliana could have at least one homologous MVD gene. In order to allow heterologous expression in S. cerevisiae, the MVD open reading frame (ORF) was then cloned under the control of the yeast PMA1 strong promoter. When expressed in yeast, the A. thaliana cDNA complemented both the thermosensitive MN19-34 strain deficient in MVD, and the lethal phenotype of an ERG19 deleted strain. However, the wild-type sterol content was not fully restored suggesting that the A. thaliana MVD activity may not be optimal in yeast. A two-hybrid assay was also performed to evaluate homodimer formation of the A. thaliana MVD and heterodimer formation between the plant and yeast heterologous enzymes.
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    The Role of the Amino-Terminal β-Barrel Domain of the α and β Subunits in the Yeast F1-ATPase (1999)
    Springer
    Journal of bioenergetics and biomembranes 31 (1999), S. 95-104 
    Details
    ISSN: 1573-6881
    Keywords: F1-ATPase ; β-barrel domain ; mitochondria ; assembly ; yeast ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The crystal structure of mitochondrial F1-ATPase indicatesthat the α and β subunits fold into a structure defined by threedomains: the top β-barrel domain, the middle nucleotide-binding domain,and the C-terminal α-helix bundle domain (Abraham et al.1994); Bianchet et al., 1998). The β-barrel domains of theα and β subunits form a crown structure at the top ofF1, which was suggested to stabilize it (Abraham et al.1994). In this study. the role of the β-barrel domain in the α andβ subunits of the yeast Saccharomyces cerevisiae F1,with regard to its folding and assembly, was investigated. The β-barreldomains of yeast F1 α and β subunits were expressedindividually and together in Escherichia coli. When expressedseperately, the β-barrel domain of the β subunit formed a largeaggregate structure, while the domain of the α subunit waspredominately a monomer or dimer. However, coexpression of the β-barreldomain of α subunit domain. Furthermore, the two domains copurified incomplexes with the major portion of the complex found in a small molecularweight form. These results indicate that the β-barrel domain of theα and β subunits interact specifically with each other and thatthese interactions prevent the aggregation of the β-barrel domain of theβ subunit. These results mimic in vivo results and suggest thatthe interactions of the β-barrel domains may be critical during thefolding and assembly of F1.
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    URL: http://dx.doi.org/10.1023/A:1005443609985
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    Saké brewing characteristics and multidrug resistance of trichothecin-resistant yeast mutants (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 629-630 
    Details
    ISSN: 1573-0972
    Keywords: Ethanol ; multi-drug resistance ; Saccharomyces cerevisiae ; trichothecin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Trichothecin-resistant mutants were isolated from saké yeast. These mutants were subjected to saké brewing, and showed a higher ethanol productivity than did the parents. They showed multidrug resistance, and resistance to organic compounds. We considered that the higher ethanol productivity of the mutants was related to their resistance to organic compounds and to their ethanol tolerance.
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    URL: http://dx.doi.org/10.1023/A:1008946001006
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    The wheat LEA protein Em functions as an osmoprotective molecule in Saccharomyces cerevisiae (1999)
    Springer
    Plant molecular biology 39 (1999), S. 117-128 
    Details
    ISSN: 1573-5028
    Keywords: LEA protein ; osmotic stress ; Saccharomyces cerevisiae ; drought ; salt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biased amino acid composition and aperiodic (random coil) configuration of Group 1 late embryogenesis-abundant (LEA) proteins imply that these proteins are capable of binding large amounts of water. While Group 1 LEAs have been predicted to contribute to osmotic stress protection in both embryonic and vegetative tissues, biochemical support has been lacking. We have used Saccharomyces cerevisiae as a model system to test the putative osmoprotective function of a wheat Group 1 LEA protein, Em. We demonstrate that expression of Em protein in yeast cells is not deleterious to growth in media of normal osmolarity and attenuates the growth inhibition normally observed in media of high osmolarity. Enhanced growth is observed in the presence of a variety of osmotically active compounds indicating that Em protein is capable of mitigating the detrimental effect of low water potential in a relatively non-specific manner. These results are the first biochemical demonstration of an osmoprotective function for a Group 1 LEA and suggest that the yeast expression system will be useful in dissecting the mechanism of protection through structure-function studies.
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    URL: http://dx.doi.org/10.1023/A:1006106906345
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    Masthead (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999) 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330101
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    Projektionsversuche mit dem Diaprojektor (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 45-50 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A variety of demonstration experiments can be shown to a large audience with a minimum amount of chemicals by using an ordinary slide projector and easily mounted cuvettes. A detailed instruction for assembling the cuvettes is given as m d l as examples for suitable demonstration experiments in general, inorganic, organic and physical chemistry.
    Additional Material: 21 Ill.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330106
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    Wer und was ist es? (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 61-61 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330111
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    Dokumentation (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 66-66 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330114
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    Rückblick (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 67-67 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330117
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    Masthead (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999) 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330201
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    Umweltprobenbanken-eine wichtige Säule der Umweltbeobachtung (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 95-103 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Natural events as well as human activities are affecting the environment and even with largescale monitoring programs it is not possible to completely assess the state of the environment. But a documentation of the environmental status with respect to its chemical composition can be realized with systematically collected representative environmental samples, which can be stored for decades at very low temperatures almost without degradation. Such ‘banking activity’ allows a current environmental monitoring and also a retrospective analysis for the determination of components which have not been analyzed at the time of sampling because of ta lack in analytical techniques or because they have not been known or considered environmentally relevant. The concept of environmental specimen banking and general procedures are described. Various examples illustrate the many facets of such activities.
    Additional Material: 13 Ill.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330205
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    Jobsuch auf dem Internet (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 128-128 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die Arbeitsmarktsituation in der Chemie hat sich in den letzten Jahren spürbar entspannt. Dennoch gibt es auf etlichen, nicht hundertprozentig dem Trend der Zeit entsprechenden Spezialgebieten immer noch einen beträchtlichen Überhang an Stellensuchenden. Entsprechende Mobilität vorausgesetzt, kann das Internet bei der Stellensuche hilfreich sein.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330210
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    Rückblick (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 135-135 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330215
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    Masthead (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999) 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330301
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    Quantenchemie auf dem Internet (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 165-166 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330306
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    Die Rolle des Aerosols im Klimasystem (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 158-164 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Atmospheric aerosol particles cause one of the largest uncertainties in estimates of human influence on climate - a good reason to take a closer look at the atmospheric life cycle of the aerosols and its effects. Besides a number of primary and secondary natural aerosol sources we need to consider since the beginning of industrialisation strong manmade particle sources. During its residence in the atmosphere the aerosol interacts in many physical and chemical ways with other atmospheric trace substances, most importantly with water vapor and its liquid and solid phases. Through its direct effect on solar and thermal radiation and through its influence on clouds the atmospheric aerosol exerts a climate forcing. To data we cannot predict the ensuing climate response because of our limited understanding of essential atmospheric processes and of the many possible feedbacks within the climate system. However, already our present knowledge of the role of the atmospheric aerosol in the climate system makes strictly global views of anthropogenic climate changes questionable.
    Notes: Der Stand unseres Wissens zum Kreislauf des Atmosphärischen Aerosols und von seiner Rolle im Klimasystem läßt sich wie folgt zusammenfassen: 1Unser Verständnis des globalen Aerosolkreislaufs und vor allem des menschlichen Eingriffs in denselben ist noch sehr unvollständig. Dies gilt in hohem Maße auch für die mit diesem Kreislauf verbundenen chemischen Mehrphasenprozesse.2Ein negativer direkter Klimaantrieb anthropogener Aerosole existiert über die Streuung und Absorption, hat aber noch große Unsicherheiten und kann über hoch mit Rußpartikeln belasteten Gebieten auch positive Werte annehmen.3Ein indirekter Klimaantrieb anthropogener Aerosole existiert wahrscheinlich und hat ebenfalls ein negatives Vorzeichen. Seine Größenordnung und Unsicherheiten lassen sich noch nicht quantifizieren.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330305
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    Welche Bedeutung haben Theoretische Konzepte in der Chemie? (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 177-185 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Chemical concepts are important to gain a qualitative understanding of chemistry. They are needed to find interesting questions to which experimental research as well as quantum-chemical investigations and computer simulations can contribute. We point out why chemical concepts should be seen as an important and from fundamental physical theories to a certain degree independent part of chemistry. An overview and analysis of some concepts - which are often introduced only in connection with special examples instead of a discussion for their own sake - are given. We take a closer look at some concepts to illustrate our assertions. An analysis of the stability of the cyclic (MoNL3)-trimer demonstrates how these concepts work and what they can tell us about this special case.
    Notes: Wir haben versucht, deutlich zu machen, wie wichtig theoretische Konzepte in der Chemie sind. Dabei gelangen wir zu folgenden Schlußfolgerungen über die Konzepte, die in der Chemie herangezogen werden, um Beobachtungen zu deuten und zu verstehen: Qualitativ chemische Konzepte sind für ein Verständnis der Chemie unverzichtbar. Eine quantenchemische Berechnung liefert nicht automatisch Verständnis und Einordnung in einen Gesamtzusammenhang.Erst die vereinfachende Beschreibung chemischer Konzepte ermöglicht eine unmittelbare Diskussion chemischer Fragestellungen.Die Konzepte sind trotz grundlegender physikalischer Theorien bis zu einem gewissen Grade eigenständig.
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330312
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    Masthead (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999) 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330401
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    Hundert Jahre Aspirin® (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 213-220 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The article commemorates the centenary of the protection of the proprietary mine of Aspirin. The early medicinal uses of salicylic acid derivatives in herbal remedies arc summarised as well as the biosynthesis of these derivatives. The history of the synthesis of salicylic acid and of acetyl salicylic acid (ASS) is described in detail as is the discovery of additional therapeutic applications and the basic pharmacology of ASS.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330406
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    Internet-Werkzeuge für die Zusammenarbeit zwischen Chemikern (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 234-235 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330409
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    Alchemie. Lexikon einer hermetischen Wissenschaft. Herausgegeben von Claus Priesner und Karin Figala. Verlag C. H. Beck, München 1998. 412 S., 40 Abb., Leinen, DM 68,-. ISBN 3-406-44104-8 (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 239-240 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330412
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    Dokumentation (1999)
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    Chemie in unserer Zeit 33 (1999), S. 246-246 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330417
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    Editorial (1999)
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    Chemie in unserer Zeit 33 (1999), S. 250-250 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330502
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    ChiuZ aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 308-309 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330508
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    Wer und was ist es? (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 312-312 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Impressum (1999)
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    Chemie in unserer Zeit 33 (1999), S. 314-314 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Literaturkarusell. Chemie (1999)
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    Chemie in unserer Zeit 33 (1999), S. IX 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Editorial (1999)
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    Chemie in unserer Zeit 33 (1999), S. 318-318 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330602
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    Chemische Biologie (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 354-358 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Chemical Biology is a rapidly growing field at the interface of organic chemistry and cell biology. Chemical strategies are used to address biological questions not amenable to traditional genetic and biochemical techniques alone. Thc enormous potential of Chemical Biology is illustrated by four recent examples.
    Notes: Chemische Biologie ist eine junge Forschungsrichtung im Grenzgebiet zwischen Organischer Cherme und Zellbiologie, welche aus einem Bedarf nach neuen Techniken zur Untersuchung zellbiologischer Mechanismen entstanden ist. Chemische Methoden werden mit biochemischen und molekularbiologischen Methoden kombiniert, um neuartige Möglichkeiten für die biologische Grundlagenforschung zu schaffen. So werden synthetische Oligosaccharide kovalent an die Oberfläche lebender Zellen gebunden, die Konzentration von Calciumionen im Zytosol künstlich reguliert und Rezeptoren künstlich aktiviert.
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    Elektrosynthesen. Strom doppelt genutzt: Erste technische „Paired Electrosynthesis“ (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 373-374 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Eine neue Elektrosynthese erfüllt erstmals den Traum der Elektrochemiker, gleichzeitig Anoden- und Kathodenreaktion für organische Synthesen technisch zu nutzen.
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    Experimente für die Chemie (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 377-377 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Persistenz und Reichweite von Umweltchemikalien. Von M. Scheringer. Wiley-VCh, Weinheim 1999.XIII + 208 Abb.,17Tab., gebunden, DM 158,-.ISBN 3-527-29752-9 (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 380-380 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Vorbereitungen für die Jahrtausend-Party (1999)
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    Chemie in unserer Zeit 33 (1999), S. 382-384 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Around the world, BASF has been preparing for Y2K since 1996. The Information services and the Process Control Technology divisions, as well as embedded systems and logistics, not to mention the company's customers and suppliers have all been systenmatically tested for Y2K capability. In addition, special precautionary measures in particular, at large Verbund sites - will be in place during the switchover period.
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    Editorial (1999)
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    Chemie in unserer Zeit 33 (1999), S. 4-4 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Andere iu Z-Zeitschriften (1999)
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    Chemie in unserer Zeit 33 (1999), S. 66-66 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ciuz.19990330115
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    Gentechnik in der Schule (1999)
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    Chemie in unserer Zeit 33 (1999), S. 104-109 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The Fonds of German Chemical Industry (FCI) has produced an instrumental kit named „Blue Genes“ which enables High School teachers to perform in their school environment basic experiments of gene analysis and cloning. In particular, the kit contains all necessary equipment and reagents to perform a restriction analysis, and cloning and expression of a bacterial gene.
    Additional Material: 7 Ill.
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    Chemie der Wunder (1999)
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    Chemie in unserer Zeit 33 (1999), S. 152-157 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: A few physical phenomena believed to be religious miracles are considered, and scientific explanations for them are proposed.
    Notes: Einige physikalische Phänomene, von denen man glaubte, sie seien religiöse Wunder, werden vorgestellt und wissenschaftliche Erklärungen hierfür vorgeschlagen.
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    Wer und was ist es? (1999)
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    Chemie in unserer Zeit 33 (1999), S. 173-173 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ciuz.19990330308
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    Lebensgeschichten. Sternstunden der frühen Chemie von Johann Rudolph Glauber bis Justus von Liebig. Von Ernst F. Schwenk. Beck'sche Reihe 1252, Verlag C. H. Beck, München 1998. 288 S., 42 s/w-Abb., Paper-back, DM 19,80. ISBN 3-406-42052-4 (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 176-176 
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    Keywords: Chemistry ; Chemistry
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    Literaturkarusell. Interdisziplinäres (1999)
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    Chemie in unserer Zeit 33 (1999), S. VIII 
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    Keywords: Chemistry ; Chemistry
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    Literaturkarusell. Pharmazie (1999)
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    Chemie in unserer Zeit 33 (1999), S. XIV 
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    Keywords: Chemistry ; Chemistry
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    Kupfer  -  Die biochemische Bedeutung eines Metalls (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 334-341 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The essential trace element copper plays a key role in living systems. Its metalloproteins are involved in various reactions. The IT ononuclear blue copper type-1 proteins take, part in electron transfer processes. Type-2 proteins can either act as oxidases, oxygenases, dioxygenases, or superoxide dismutases, containing one copper core. Dinuclear copper centers of Type-3 proteins are the oxygen transport system hemocyanin, oxidases and oxygenases. Due to this variety of functions copper deficiency and also abundance can cause severe damage of any life form.
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    Fritz Haber, Carl Bosch und Friedrich Bergius  -  Protagonisten der Hochdrucksynthese (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 359-364 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: It is tried to fix the limits between the notions high pressure synthesis and high temperature chemistry. The work of Fritz Haber to apply high pressure to synthetise ammonia and of Friedrich Bergius to liquify coal to synthetise petrol is described. Special attention is given to the merits of Carl Bosch and his coworkers at BASF for applying the high pressure synthesis into industrial scale. The consequences of thc work of Haber, Bergius und Bosch on chemistry, social and political life and their importance for the present are considered.
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    Bakterien, Viren, Prionen (1999)
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    Chemie in unserer Zeit 33 (1999), S. 376-376 
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    Dokumentation (1999)
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    Chemie in unserer Zeit 33 (1999), S. 390-390 
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    Keywords: Chemistry ; Chemistry
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    URL: http://dx.doi.org/10.1002/ciuz.19990330622
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    Von Biomineralien zu biomimetischen Materialien: Der Weg ist das Ziel (1999)
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    Chemie in unserer Zeit 33 (1999), S. 6-19 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Diatoms are unicellular organisms which possess a unique silicified cell wall. Their intricate structures have inspired the imagination of naturalists and scientists throughout centuries. However, molecular biological details about silica metabolic pathways still are obscure. To find out how soft matter can selforganize and create finite solid shapes and complex patterns is certainly one of the most challenging scientific problems today. Especially chemists just have started to unravel the mysteries of chemical morphogenesis.
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    ChiuZ aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 58-60 
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    Keywords: Chemistry ; Chemistry
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    Chemie im Selbststudium. Grundkurs Chemie I. Allgemeine und Anorganische Chemie. Von Arnold Arni. überarb. Aulf. 3., überarb. Aufl., WILEY-VCH, Weinheim 1998. XIV + 350 S., 66 Abb., 15 Tab., Broschur, DM 54,-. ISBN 3-527-29542-9. Grundkurs Chemie II. Organische Chemie. 2., überarb. Aufl. WILEY-VCH, Weinheim 1998. XIII + 198 S., 16 Abb., 5 Tab., Broschur, DM 48,-. ISBN 3-527-29562-3 (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 60-61 
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    Keywords: Chemistry ; Chemistry
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    Impressum (1999)
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    Chemie in unserer Zeit 33 (1999), S. 66-66 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    URL: http://dx.doi.org/10.1002/ciuz.19990330116
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    Editorial (1999)
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    Chemie in unserer Zeit 33 (1999), S. 70-70 
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    URL: http://dx.doi.org/10.1002/ciuz.19990330202
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    Wissenschaft aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 122-125 
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    Dokumentation (1999)
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    Chemie in unserer Zeit 33 (1999), S. 134-134 
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    URL: http://dx.doi.org/10.1002/ciuz.19990330212
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    Impressum (1999)
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    Chemie in unserer Zeit 33 (1999), S. 134-134 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Literaturkarusell. Chemie (1999)
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    Chemie in unserer Zeit 33 (1999), S. X 
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    Editorial (1999)
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    Chemie in unserer Zeit 33 (1999), S. 138-138 
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    ChiuZ aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 174-175 
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    Chemie mit Licht. Photochemie. Von D. Wöhrle, M.W. Tausch und W.-D. Stohrer. WILEY-VCH, Weinheim 1998. XVI, 253, S., 169 Abb., 39 Tab., Broschur, DM 128,-.ISBN 3-527-29545-3 (1999)
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    Chemie in unserer Zeit 33 (1999), S. 175-176 
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    Editorial (1999)
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    Chemie in unserer Zeit 33 (1999), S. 190-190 
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    Sandwich-artige Cyclopentadienylkomplexe von Hauptgruppenelementen (1999)
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    Chemie in unserer Zeit 33 (1999), S. 342-353 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Cyclopentadienyl (Cp) complexes of maingroup elements are classified and compared in terms of structural types, bonding features, and characteristic reactions. Halfsandwich, sandwich and multidecker sandwich arrangements of different type are observed including cluster species with weak or strong metal-metal bonds. The Cp-El bonding ranges from mainly covalent to purely ionic with important structure-determining intermolecular contacts in the latter. π-Complexation allows the stabilisation of compounds with low-valent elements such as AI(I), Ga(I), and Si(I1). Important reaction principles and applications are shortly described.
    Additional Material: 19 Ill.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330605
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    Wissenschaft aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 367-372 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330608
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    ChiuZ aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 375-375 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330612
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    Schreiben und publizieren in den naturwissenschaften. Vin H.F.Ebel u C. Bliefert. 4., völlig neu bearb. Aufl., WILEY-VCh, Weinheim 1988.XIV + 550 s., Broschur, Dm 68,-ISBN 3-527-29626-3 (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 378-379 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330615
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    Wer war es? (1999)
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    Chemie in unserer Zeit 33 (1999), S. 381-381 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330620
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    Andere iu Z-Zeitschriften (1999)
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    Chemie in unserer Zeit 33 (1999), S. 390-390 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330623
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    Wissenschaft aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 53-58 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330107
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    Koffeinschub für Chemie-Applets im WWW (1999)
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    Chemie in unserer Zeit 33 (1999), S. 62-63 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330112
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    Wer war es? (1999)
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    Chemie in unserer Zeit 33 (1999), S. 129-129 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330211
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    Andere iu Z-Zeitschriften (1999)
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    Chemie in unserer Zeit 33 (1999), S. 134-134 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330213
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    Literaturkarusell. Biologie (1999)
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    Chemie in unserer Zeit 33 (1999), S. V 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330216
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    Literaturkarusell. Physik (1999)
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    Chemie in unserer Zeit 33 (1999), S. VIII 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330217
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    Impressum (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 186-186 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330315
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    Methyltrioxorhenium (1999)
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    Chemie in unserer Zeit 33 (1999), S. 192-198 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330403
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    Molekulare Aspekte des Alterns (1999)
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    Chemie in unserer Zeit 33 (1999), S. 221-225 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In the last decade, major advances in the understanding of the biology of aging were made. Studies in worms, flies and yeast led to the identification of several life-extending genes. In mammals, two genes were found which, when mutated, shorten life span. Previous studies in rodents reporting life span prolongation by caloric restriction were confirmed and extended to lower organisms. The studies arc consistent with and supportive of the free radical theory of aging, which secs reactive oxygen species as the main contributor to aging. A refinement of this theory puts mitochondrial DNA into the center of the aging process. It appears that mitochondrial DNA may be both, a yardstick for the biological age and a signalling device to tell the organism when to die. A diet low in calories and high in antioxidants promises to lead to a better quality of life at old age and possibly to increased life span for humans.
    Additional Material: 2 Tab.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330407
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    ChiuZ aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 237-239 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330411
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    Radikale und Radikalionen in der Organischen Synthese. Von Torsten Linker und Michael Schmittel. WILEY-VCH, Weinheim 1998. 421 S., DM 88,-. ISBN 3-527-29492-9 (1999)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 33 (1999), S. 241-241 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330415
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    Impressum (1999)
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    Chemie in unserer Zeit 33 (1999), S. 247-247 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/ciuz.19990330419
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    Wiederaufladbare Batterien (1999)
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    Chemie in unserer Zeit 33 (1999), S. 252-266 
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    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This first part of „Rechargeable Batteries“ is devoted to materials and reactions in batteries with aqueous electrolytes. Lead-acid, nickelcadmium, nickel-metal hydride, and various zinc systems, such as zinc-manganese oxide, zinc-air, and zinc-bromine cells are discussed. Special attention is paid to the limited electrochemical stability window of the aqueous electrolytes, which enables overcharge and overdischarge protection of the battery but limits cell voltages to less than 2 Volts. In the following part of this article we will focus on nonaqueous electrolyte-based rechargeable batteries. Due to thc wider electrochemical stability window of these electrolytes high specific charge and high cell voltage batteries can be realized, e.g., the „4 V“ lithium ion cell.
    Additional Material: 19 Ill.
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    URL: http://dx.doi.org/10.1002/ciuz.19990330503
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    Wissenschaft aktuell (1999)
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    Chemie in unserer Zeit 33 (1999), S. 304-308 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19990330507
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