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  • 1
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    Combined proton NMR imaging and spectral analysis of locust embryonic development (1987)
    National Academy of Sciences
    Details
    Publication Date: 1987-08-01
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Fusion of cultured dog kidney (MDCK) cells: I. Technique, fate of plasma membranes and of cell nuclei (1989)
    Springer
    The journal of membrane biology 111 (1989), S. 37-48 
    Details
    ISSN: 1432-1424
    Keywords: Cell membrane fusion ; cell nuclei fusion ; cell culture ; MDCK cells ; lectins ; acridine orange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The evaluation of the intracellular signal train and its regulatory function in controlling transepithelial transport with electrophysiological methods often requires intracellular measurements with microelectrodes. However, multiple impalements in epithelial cells are hampered by the small size of the cells. In an attempt to avoid these problems we fused cells of an established cell line, Madin Darby canine kidney cells, originally derived from dog kidney, to “giant” cells by applying a modified polyethylene glycol method. During trypsin-induced detachment from the ground of the petri dish, individual cells grown in a monolayer incorporate volume and mainly lose basolateral plasma membrane by extrusion. By isovolumetric cell-to-cell fusion, spherical “giant” cells are formed within 2 hr. During this process a major part of the individual cell plasma membranes is internalized. Over three weeks following cell plasma membrane fusion degradation of single cell nuclei and cell nuclear fusion occurs. We conclude that this experimental approach opens the possibility to investigate ion transport of epithelia in culture by somatic cell genetic techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869207
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  • 3
    Electronic Resource
    Electronic Resource
    Proton NMR in Vivo Imaging: A Tool for Studying Embryo Development of Small Organisms (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 508 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb32929.x
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  • 4
    Electronic Resource
    Electronic Resource
    [1-(Aryl-bzw. Alkylcarbonyloxy)alkyl]phosphonium-Salze, 4. Herstellungsmethoden (1985)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 118 (1985), S. 124-131 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: [1-(Aryl- and Alkylcarbonyloxy)alkyl]phosphonium Salts, 4 Synthetic MethodsFour syntheses A  -  D are described for the phosphonium salts 1, which are precursors of the highly reactive phosphoranes 3. Whereas the usual method B fails in some cases it is now possible to prepare 1 with a wide variety of substitutents R1, R2, and R3 and anions X-.
    Notes: Für die Phosphoniumsalze 1, Vorstufen der hochreaktiven Phosphorane 3, werden vier Synthesemöglichkeiten A  -  D beschrieben. Während das bisherige Verfahren B in einigen Fällen versagt, sind die Salze 1 nunmehr bei großer Variationsbreite der Reste R1, R2 und R3 sowie der Anionen X- herstellbar.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19851180113
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  • 5
    Electronic Resource
    Electronic Resource
    Chemische Modifikation von Kanamycin A Derivate mit 4,5-Doppelbindung im Kanosamin-Teil (1985)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1985 (1985), S. 644-649 
    Details
    ISSN: 0170-2041
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Chemical Modification of Kanamycin A. - Derivatives Containing a 4,5-Double Bond in the Kanosamine PartOxidation of the primary alcohol group in the kanamycin A derivative 2b with simultaneous β-elimination gave the α,β-unsaturated aldehyde 3a of which the derivatives 3b - e were prepared by acetalisation, oxidation, or reduction. Hydrogenation of the double bond led to 4 ‚-deoxy-5‘-epi-kanamycin A derivatives.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.198519850327
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  • 6
    Electronic Resource
    Electronic Resource
    A measuring grid for the analysis of isoelectrically focused proteins in polyacrylamide gel slabs (1985)
    Weinheim : Wiley-Blackwell
    Electrophoresis 6 (1985), S. 206-209 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: An overlay grid was constructed to facilitate the measurement of densiometric scans of proteins in pH gradients formed by electrofocusing. The overlay procedure provides a rapid way for data collection. The relationship between pH and gel distance closely fit a straight line, (R 〉0.99). The reliability of the system was shown by the close agreement of the linear regressions from six different sets of data. Also, the feasibility of using a similar grid for a nonlinear system was established.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150060504
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  • 7
    Electronic Resource
    Electronic Resource
    N-[1-(Acyloxy)alkyl]heteroaryliumsalze in der Synthese, 3. Intramolekulare ortho-Acylierung einiger N-heteroaromatischer Ringsysteme (1986)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 119 (1986), S. 279-296 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: N[1-(Acyloxy)alkyl]heteroarylium Salts in Synthesis, 3. Intramolecular ortho-Acylation of Some N-Heteroaromatic Ring SystemsThe title compounds 17 (prepared by methods A—C) have been reacted with sodium bis(trimethylsilyl)amide (18) in a heterogeneous mixture (-80°C, 2-60 h). The ortho-substituted N-heteroaromatic compounds 4 are isolated exclusively after an intramolecular and regiospecific reaction [Scheme 1, Eq. (5)]. This method represents the first widely applicable acylation technique for N-heteroaromatic compounds in which the incoming substituent (R1CO) functions as an internal electrophile and in which the formation of isomeric or polyacylated products has not yet been observed. Both experimental and theoretical (MNDO) investigations show that the substitution reaction (5) is primarily determined by the conformational and electronic properties of the deprotonation product 25 [Eq. (7)], which is formed preferentially.
    Notes: Die Titelverbindungen 17 (Herstellungsmethoden A—C) werden in heterogener Phase mit Natrium-bis(trimethylsilyl)amid (18) umgesetzt (-80°C, 2-60 h). Nach einem intramolekular und regiospezifisch verlaufenden Folgeschritt werden ausschließlich die ortho-substituierten N-heteroaromatischen Verbindungen 4 isoliert [Schema 1, Gl. (5)]. Diese Methode repräsentiert das erste breit anwendbare Acylierungsverfahren in der N-heteroaromatischen Reihe, bei dem der hinzukommende Substituent (R1CO) als internes Elektrophil eingesetzt wird und die Entstehung isomerer oder mehrfach acylierter Substitutionsprodukte bisher nicht beobachtet wurde. Aus experimentellen und theoretischen Untersuchungen (MNDO-Methode) folgt übereinstimmend, daß der Verlauf der Substitutionsreaktion (5) maßgeblich von den konformativen und elektronischen Eigenschaften des bevorzugt entstehenden Deprotonierungsprodukts 25 [Gl. (7)] bestimmt wird.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19861190125
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  • 8
    Electronic Resource
    Electronic Resource
    Mechanistische Untersuchungen zur dephosphorylierenden Ylid-Diketon-Umlagerung der [(Arylcarbonyloxy)alkyliden]phosphorane (1986)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 119 (1986), S. 1350-1360 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Mechanistic Studies on the Dephosphorylating Ylide-Diketone Rearrangement of [(Arylcarbonyloxy)alkylidene]phosphoranesA new rearrangement of the phosphoranes 8 to 1,2-diketones 15 can be classified as an intramolecular 3-exo-trig process on the basis of experimental results. The mechanism is discussed in terms of a polar (Route a) or alternatively a carbene (Route b) reaction. The transition state for carbene formation from the model compound 17 [(hydroxymethylene)- phosphorane] has been determined using the ab-initio RHF/6-31G*/6-31G* method. The results indicate that π-donor/σ-acceptor-substituted phosphoranes 1a and 8 can be considered as potential carbene precursors by analogy with the model compounds 16 and 17.
    Notes: Die zu den 1,2-Diketonen 15 führende neue Umlagerung der Phosphorane 8 wird auf der Basis experimenteller Ergebnisse als intramolekularer 3-exo-trig-Prozeß klassifiziert und alternativ im Sinne eines polaren (Weg a) und carbenischen (Weg b) Mechanismus diskutiert, Der Übergangszustand der Carbenbildung aus einer charakterisierenden Modellverbindung 17 [(Hydroxymethylen)phosphoran] wurde ab-initio nach der RHF/6-31G*/6-31G*-Methode mit dem Resultat berechnet, daß π-Donor/σ-Donor/σ-Akzeptor-substituierte Phosphorane 1a, 8 bzw. deren Modelle 16 und 17 als potentielle Carben-Vorstufen zu interpretieren sind.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19861190422
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