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  • 1
    Monograph available for loan
    Monograph available for loan
    Beiträge zur Petrographie und Geologie des Maggia-Lappens (NW- Tessin) : I. Petrographie des oberen Val Peccia ; II. Tektogenese des Maggia-Lappens (1954)
    Zürich
    Details Availability
    Call number: MR 90.0577
    Type of Medium: Monograph available for loan
    Pages: 159 S.
    Language: German
    Location: Upper compact magazine
    Branch Library: GFZ Library
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  • 2
    Series available for loan
    Series available for loan
    Ultrafiltration zur Trinkwasseraufbereitung (2004)
    München : Oldenbourg Industrieverl.
    Associated volumes
    Details Availability
    Call number: ZS-142(93)
    In: Mitteilungen
    Type of Medium: Series available for loan
    Pages: Getr. Zählung
    ISBN: 3486630679
    Series Statement: Mitteilungen / Institut für Wasserwesen, Universität der Bundeswehr München 93
    Classification:
    Hydrology
    Location: Lower compact magazine
    Branch Library: GFZ Library
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  • 3
    Series available for loan
    Series available for loan
    Feststoffabtrennung auf Kläranlagen : Theorie und Praxis (2001)
    München : Oldenbourg Industrieverl.
    Associated volumes
    Details Availability
    Call number: ZS-142(78)
    In: Mitteilungen
    Type of Medium: Series available for loan
    Pages: 148 S.
    ISBN: 3486265253
    Series Statement: Mitteilungen / Institut für Wasserwesen 78
    Language: German
    Location: Lower compact magazine
    Branch Library: GFZ Library
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  • 4
    Electronic Resource
    Electronic Resource
    Inactivation of PR8 influenza virus through the octadecylrhodamine B chloride membrane marker (1993)
    s.l. : American Chemical Society
    Biochemistry 32 (1993), S. 900-907 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00054a022
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  • 5
    Electronic Resource
    Electronic Resource
    Über das Alpine Alter der Penninischen Deckengesteine des W Tessins und der Angrenzenden Simplon-Region (1956)
    Springer
    International journal of earth sciences 45 (1956), S. 194-202 
    Details
    ISSN: 1437-3262
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Zusammenfassung Argand, Preiswerk und neuerdings auchNabholz halten die granitoiden Kerngesteine der penninischen Decken der Simplon-Tessin Region (schweizerische und italienische Alpen) für herzynische Eruptivgesteine, die alpin mehr oder weniger vergneist wurden.Nabholz gliedert das penninische Kristallin in einen vorherzynischen und einen herzynischen vortriadischen Gesteinsanteil. Neuere Untersuchungen belegen jedoch das alpine Alter der penninischen Deckengesteine der genannten Region und lassen lediglich eine Gliederung in alpin ältere und alpin jüngere Deckengesteine zu. Die älteren werden Hüllgesteine genannt; aus ihnen sind durch alpin syn-bis posttektonische Granitisation s. 1. die jüngeren Kerngesteine hervorgegangen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01802006
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  • 6
    Electronic Resource
    Electronic Resource
    Formation of Multilayers in the Caco-2 Cell Culture Model: A Confocal Laser Scanning Microscopy Study (2000)
    Springer
    Pharmaceutical research 17 (2000), S. 460-465 
    Details
    ISSN: 1573-904X
    Keywords: Caco-2 ; in vitro intestinal epithelial model ; confocal laser scanning microscopy ; tight junctions ; cytoarchitecture ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To introduce confocal laser scanning microscopy (CLSM)combined with digital image restoration to characterise Caco-2 cellsunder different culture conditions, and thus to define additional validcriteria for the optimisation of culture models. Methods. Growth curves were established and transepithelial electricalresistance (TEER) measured for cells grown in EMEM or DMEMmedium on Cyclopore™ membranes. Cytoskeleton, cell nuclei and tightjunctions (TJ) were investigated by CLSM. Results. Cultures reached a plateau of ∼4.5 × 105 cells/cm2 after∼ 10 days. At the same time TEER reached 750 Ω cm2. An irregular,fairly complete network of TJ was present at confluence (∼2 d).Between 15 and 30 days a regular TJ network was established. Cellsformed mixed mono- and multilayers under most conditions with twoexceptions: flat monolayers were observed on polycarbonate filterswith EMEM and with the Biocoat™ intestinal epithelium differentiationenvironment system. In multilayers TJ were found in the upper aswell as in the lower cell layers although the regular vertical polaritywas disturbed. Conclusions. CLSM represents an important tool to investigate thecytoarchitecture of Caco-2 cells. 3D-analysis of confocal data givesimportant clues on the characteristics of cell layers and thus helps tovalidate optimisation strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007585105753
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  • 7
    Electronic Resource
    Electronic Resource
    MDCK Cell Cultures as an Epithelial In Vitro Model: Cytoskeleton and Tight Junctions as Indicators for the Definition of Age-Related Stages by Confocal Microscopy (1998)
    Springer
    Pharmaceutical research 15 (1998), S. 964-971 
    Details
    ISSN: 1573-904X
    Keywords: MDCK cells ; confocal laser scanning microscopy ; cytoskeleton ; tight junctions ; in vitro epithelial cell model ; age-related changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. Madin Darby Canine Kidney (MDCK) cells were grown in culture, and age-related morphological changes in the cytoskeleton and tight junction (TJ) network were used to define stages in view of establishing an optimal in vitro model for the epithelial barrier. Methods. Growth curves and transepithelial electrical resistance (TEER) were determined, and the cytoskeleton (actin, α-tubulin, vimentin) and TJ (Zonula occludens proteins ZO1, ZO2) were investigated with immunofluorescent methods by confocal laser scanning microscopy (CLSM) and digital image restoration. Results. TEER measurements indicated that TJ were functional after one day. Values then remained constant. Four morphological stages could be distinguished. Stage I (0−1 day): Sub confluent cultures with flat cells; TJ established after cell-to-cell contacts are made. Stage II (2−6 days): Confluent monolayers with a complete TJ network, which remains intact throughout the later stages. Stage III (7−14 days): Rearrangement in the cytoskeleton; constant cell number; volume and surface area of cells reduced (cobble-stone appearance). Stage IV (≥ 15 days): Dome formation, i.e. thickening and spontaneous uplifting of the cell monolayer. Conclusions. Based on the structural characteristics of stage III cell cultures, which are closest to the in vivo situation, we expect them to represent an optimal in vitromodel to study drug transport and/or interactions with drugs and excipients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011953405272
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  • 8
    Electronic Resource
    Electronic Resource
    Microwave irradiation of paraffin-embedded tissue sensitizes the TUNEL method for in situ detection of apoptotic cells (1995)
    Springer
    Histochemistry and cell biology 103 (1995), S. 157-160 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Apoptosis is a morphologically distinct form of programmed cell death that plays an important role in the growth regulation of a variety of tissues and also in the elimination of self-reacting immunocompetent cells. Several techniques for the qualitative and quantitative detection of this process have been established; recently, an in situ nick end-labelling technique based on the detection of DNA fragmentation, which is a molecular characteristic of apoptotic cell death, was described. Applying this method to paraffin sections of human tissues, sensitivity was observed to be inconsistently low with regard to the expected number of apoptotic cells. In the present study we show that irradiation of the tissue sections in 10 mM citrate buffer, pH 6.0, by microwaves at 750 W considerably enhances the sensitivity of this nick end-labelling technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01454013
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  • 9
    Electronic Resource
    Electronic Resource
    CD44 variant isoforms are preferentially expressed in basal epithelia of non-malignant human fetal and adult tissues (1994)
    Springer
    Histochemistry and cell biology 101 (1994), S. 79-89 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract CD44 is a transmembrane glycoprotein, which can exist in a multitude of isoforms due to alternative splicing of the pre-mRNA. We have generated monoclonal antibodies to several of these variant regions, which are encoded by 10 additional exons in the extracellular part of the molecule. CD44 variant isoforms have been reported to be involved in the malignant progression of rat and human tumours. The precise localization of CD44 variant isoforms in normal developmental and morphogenetic processes is essential for diagnostic studies of human tumorigenesis. Therefore, we have analysed a large number of different human tissues by immunohistochemistry for the expression of CD44 isoforms containing either exons 4v, 6v or 9v. Expression of exon 9v-isoforms was detected in almost all epithelia analysed, with a few exceptions. Exon 6v isoforms are expressed only in squamous and glandular epithelia, e.g. skin epidermis, sweat and sebaceous glands, oesophagus, ducts of the mammary gland, salivary and prostate glands. Detection of exon 4v-encoded isoforms was restricted to the epidermis and the oesophagus. Similar tissue distributions of CD44 variant isoforms were observed in 10-week-old fetal tissues. Since one of the ligands of CD44 is hyaluronic acid (HA), we also analysed the tissue distribution of HA synthetase. HA synthetase was detected in all tissues analysed, showing good correlation with the expression of the standard form of CD44, CD44s.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269353
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  • 10
    Electronic Resource
    Electronic Resource
    CD44 standard and variant isoform expression in normal human skin appendages and epidermis (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 283-289 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract CD44 isoforms have been implicated in tumor progression and metastasis formation. This study presents a thorough immunohistochemical analysis of CD44 standard ann isoform expression in normal human skin appendages and epidermis applying monoclonal antibodies against CD44s, CD44v3,-v4,-v5,-v6, and-v9. An improved immunohistochemical protocol with microwave-based antigen retrieval in paraffin section and heavy metal amplificatio of the diaminobenzidine reaction product provided enhanced resolution and sensitivity as compared to studies on frozen sections. The hair follile, the seborrheic and eccrine sweat glands were strongly positive for all CD44 isoforms studied. In the latter, the clear cells but not the dark (intercalated) cells were positive. The sudoriferous ducts adjacent to the glands were weakly positive for all CD44 isoforms and strongly positive near the skin surface. In the apocrine glands, the basal cells showed only a moderate positivity. The myoepithelial cells expressed only CD44s. In the epidermis, all CD44 isoforms were detectable, with strongest CD44 immunostaining in the lower third of the stratum spinosum and weaker staining in the stratum basale and the upper two-thirds of the stratum granulosum. The stratum granulosum and corneum were unreactive. Thus, a regional and cell type-specific CD44 expression was revealed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02473238
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