ISSN:
1432-203X
Schlagwort(e):
Key wordsAgrobacterium tumefaciens
;
Pea transformation
;
Cotyledonary meristem
;
Bar gene
;
Phosphinothricin resistance
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens-mediated trans-formation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01142315
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