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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 64 (1982), S. 47-50 
    ISSN: 1432-2242
    Keywords: Lipid content ; Storage protein ; Legumeseeds ; Genetic control ; Pea ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The lipid content of seed from a set of isogenic lines for the R a : r a locus has been determined; the results show that this locus as well as affecting the starch, sugar and storage protein content and composition, also has a marked effect on the lipid content of the seed. Genotypes having different combinations of alleles at the r a and r b loci have also been examined; an r a r a r b r b , genotype had 5.57% purified total lipid in its seed — a more than 2-fold increase over that found in the round-seeded varieties (R a R a R b R b ) usually grown for the dry sed crop.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 16 (1997), S. 513-519 
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium tumefaciens ; Pea transformation ; Cotyledonary meristem ; Bar gene ; Phosphinothricin resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens-mediated trans-formation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 16 (1997), S. 513-519 
    ISSN: 1432-203X
    Keywords: Agrobacterium tumefaciens ; Pea transformation ; Cotyledonary meristem ; Bar gene ; Phosphinothricin resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1988), S. 362-365 
    ISSN: 1432-2242
    Keywords: Pea ; r b ; RFLP ; Vicilin gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have located an RFLP marker, corresponding to the locus Vc-5, which is linked to the r b locus. We also show that the heterogeneity at the Vc-5 locus is less among r brb lines than among pea genotypes as a whole. The relevance of this RFLP is discussed in relation to the construction of the double recessive rr r brb genotypes.
    Type of Medium: Electronic Resource
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