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A simple system for pea transformation (1997)

Bean, S. J. ; Gooding, P. S. ; Mullineaux, P. M. ; [et al.]

Springer

Plant cell reports 16 (1997), S. 513-519

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ISSN: 1432-203X

Keywords: Key wordsAgrobacterium tumefaciens ; Pea transformation ; Cotyledonary meristem ; Bar gene ; Phosphinothricin resistance

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens-mediated trans-formation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01142315

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