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  • Articles  (146)
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  • 1
    Electronic Resource
    Electronic Resource
    Glutamate dehydrogenase regulation in callus cultures of Nicotiana plumbaginifolia: effect of glucose feeding and carbon source starvation on the isoenzymatic pattern (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 14 (1991), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. In callus cultures of Nicotiana plumbaginifolia, the activity of glutamate dehydrogenase was repressed by glucose, whereas, on the contrary, carbon and energy source deprivation induced a remarkable increase in specific activity. Definition of these two opposite types of response was made possible by the use of glycerol as a non-repressing carbon source: in this condition, glutamate dehydrogenase activity reached an intermediate level, which was similar to the derepressed values of activity obtainable when cultures were allowed to exhaust the glucose supply in the medium. Isoelectric focusing analysis revealed the existence of three different isoenzymatic patterns which could be correlated to the three different levels of specific activity: repressed (glucose), induced (carbon starvation) and intermediate (glycerol). Repression affected mainly the four more cathodic bands which were predominant in non-repressed conditions. The possible catabolic role of these isoenzymes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1991.tb01533.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ertyhromycin as a tool for discriminating in vivo between mitochondrial and chloroplastic protein synthesis in Nicotiana sylvestris
    Amsterdam : Elsevier
    Plant Science Letters 29 (1983), S. 215-225 
    Details
    ISSN: 0304-4211
    Keywords: Chloroplastic protein synthesis ; Erythromycin ; Mitochondrial protein synthesis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0304-4211(83)90146-3
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  • 3
    Electronic Resource
    Electronic Resource
    The effects of carbon starvation on cellular metabolism and protein and RNA synthesis in Gerbera callus cultures
    Amsterdam : Elsevier
    Plant Science 83 (1992), S. 127-136 
    Details
    ISSN: 0168-9452
    Keywords: Gerbera jamesonii ; glutamate dehydrogenase induction ; heat shock proteins ; starvation-related proteins ; translational/transcriptional regulation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(92)90070-3
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  • 4
    Electronic Resource
    Electronic Resource
    Sex determination and differentiation in Asparagus officinalis L.
    Amsterdam : Elsevier
    Plant Science 80 (1991), S. 67-77 
    Details
    ISSN: 0168-9452
    Keywords: Asparagus officinalis ; dioecy ; sex
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(91)90273-B
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  • 5
    Electronic Resource
    Electronic Resource
    Isozyme gene markers in the dioecious species Asparagus officinalis L. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 613-618 
    Details
    ISSN: 1432-2242
    Keywords: Asparagus officinalis ; Isoenzymes ; Marker genes ; Sex-linked inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Extracts from phylloclads of Asparagus officinails were electrophoretically analyzed for isozyme polymorphism. Fourteen enzyme systems were examined using four buffer systems: seven enzymes (acid phosphatase, catalase, glutamate-oxaloacetate transaminase, isocitrate dehydrogenase, malate dehydrogenase, peroxidase, and 6-phosphogluconate dehydrogenase) exhibited clear and consistent banding patterns. Isozyme polymorphism was studied in seven pairs of male and female doubled haploids and in their male F1s. Segregation of polymorphic loci was examined in the backcross progenies and was found to be consistent with a simple Mendelian inheritance in all cases, except for three anodical peroxidases, where two factors have been hypothesized. No linkage could be found between isozyme markers that were segregating in the same cross, but association was demonstrated between one malate dehydrogenase locus and the sex determining genes. The availability of isozyme markers may be useful in breeding and, in particular, the localization of one malate dehydrogenase locus on the sex chromosomes may be helpful in mapping the sex genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226726
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  • 6
    Electronic Resource
    Electronic Resource
    A genetic map of Asparagus officinalis based on integrated RFLP, RAPD and AFLP molecular markers (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1083-1089 
    Details
    ISSN: 1432-2242
    Keywords: Key words Asparagus ; Dioecious species ; Genetic map ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An integrated genetic map of the dioecious species Asparagus officinalis L. has been constructed on the basis of RFLP, RAPD, AFLP and isoenzyme markers. The segregation analysis of the polymorphic markers was carried out on the progeny of five different crosses between male and female doubled-haploid clones generated by anther culture. A total of 274 markers have been organized to ten linkage groups spanning 721.4 cM. Since the haploid chromosome number of asparagus is ten, the established linkage groups probably represent the different chromosomes; however, the only group associated with a specific chromosome is the one which includes sex, whose determinant genes have been located on chromosome 5. A total of 33 molecular markers (13 RFLPs, 18 AFLPs, 2 RAPDs and 1 isoenzyme) have been located on this chromosome. The closest marker to the sex determinant is the AFLP SV marker at 3.2 cM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050995
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  • 7
    Electronic Resource
    Electronic Resource
    Linkage Arrangement of RFLP loci in progenies from crosses between doubled haploid Asparagus officinalis L. clones (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 124-128 
    Details
    ISSN: 1432-2242
    Keywords: Asparagus officinalis L ; Genetic map RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A preliminary genetic map of the dioecious species Asparagus officinalis L. (2n = 20) has been constructed on the basis of restriction fragment length polymorphism (RFLP) and isozyme marker data. With DNA samples digested with either EcoRI or HindIII 61 out of 148 probes (41%) identified RFLPs in six families of doubled haploid lines obtained through anther culture. A higher level of polymorphism (65%) was observed when a single family was screened for RFLPs using six distinct restriction enzymes. Segregation analysis of the BC progenies (40–80 individuals) resulted in a 418-cM extended map comprising 43 markers: 39 RFLPs, three isozymes and one morphological (sex). These markers are clustered in 12 linkage groups and four of them exhibited significant deviations from the expected 1∶1 ratio. One isozyme and three RFLP markers were assigned to the sex chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221005
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of rho mutability in Saccharomyces cerevisiae (1980)
    Springer
    Molecular genetics and genomics 177 (1980), S. 589-595 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Erythromycin induced mutability of the cytoplasmic factor rho in S. cerevisiae does not depend only on the participation of its mitochondrial target (formally a mitochondrial protein synthesis component) to the replication of the mitochondrial DNA. The relative increase from the spontaneous background in the percentage of RD mutants accumulated in the presence of the drug is the same in mmc and MMC strains whereas the total number of RD mutants accumulated is about tenfold higher in mmc strains. Mutations at the mitochondrial locus that controls the sensitivity to Erythromycin reduce both the Erythromycin induced and the spontaneous mutability of rho in mmc strains indicating that the gene products of nuclear (MMC) and mitochondrial (ERYs) determinants interact in determining the fidelity of a “replicative complex” for the mitochondrial DNA. The fact that ERYR allelesaare able to suppress the pet-ts associated mutability of rho but not the RD conditional trait of these mutants makes evident that the pet-ts gene-products have an additional role beside the enzymatic or the biosynthetic ones. Since the pet-ts genes are not directly involved in the metabolism of mitochondrial DNA we hypothesize that they provide for some structures that contribute to ensure the proper conformation of a “replicative complex” for mitochondrial DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272668
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  • 9
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    Fluid geochemistry versus tectonic setting: the case study of Morocco (2007)
    In:  Geological Society Special Publication 262: 131-145.
    Details
    Publication Date: 2007-10-08
    Description: This paper presents the results of an extensive chemical and isotopic investigation on natural thermal and cold fluids (spring waters and associated gases) discharging throughout the main geological domains of Morocco. The chemical features of the thermal springs are mainly dependent on rock dissolution involving Triassic evaporite formations, producing either Na-Cl or Ca-SO4 composition, although mixing with shallower connate high-saline waters in Neogene post-orogenic sedimentary layers cannot be ruled out. Only in the Moroccan Meseta and Anti-Atlas domains have spring discharges probably undergone equilibration as a result of water-rock interaction in granites. Of the chemical and isotopic features of the gas seeps, 3He/4He ratios and {delta}13C-CO2 values indicate the occurrence of a significant contribution of mantle-derived gas, especially at Oulmes (Moroccan Meseta) and Tinejdad-Erfoud (Anti-Atlas), where associated waters are found to equilibrate at relatively high temperatures (c. 130 {degrees}C). These areas are also characterized by the presence of Pliocene to Quaternary basaltic volcanic rocks. Thermal discharges located along the Rif front and related to the NE-SW-oriented main strike-slip faults are associated with a CH4- and/or N2-rich gas phase, derived respectively from a crustal or an atmospheric source. Some of them have significant contents of 3He that could indicate the rising of mantle fluids. Such a striking isotopic signature, which is not related to any recent volcanism visible at surface, is likely to be associated with cooling magma at depth related to transpressive fault systems. Similarly, in the northeastern area, the small, although significant, enrichment of 3He in the gas discharges seeping out along the Nekor seismic active fault and related to Pliocene-Quaternary basalts also suggests a deep-seated (magmatic) contribution. The distribution of thermal discharges is strongly related to the main active tectonic structures of Morocco. Moreover, this study indicates the presence of deep active tectonic structures in areas until now considered as stable. In particular, the NE-SW-trending Nekor fault may be part of a major system that extends to the Moroccan Meseta and into the Smaala-Oulmes fault system, thus emerging as a deep structure with crustal significance.
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  • 10
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    Compositional changes in a fumarolic field, Vulcano Island, Italy: a statistical case study (2007)
    In:  Geological Society Special Publication 264: 67-77.
    Details
    Publication Date: 2007-10-08
    Description: The identification of compositional changes in fumarolic gases of active volcanic areas is one of the most important objectives in monitoring programmes. Together with information from seismic data and deformation, it provides key data to the formulation and management of emergency plans for populations living near active volcanoes. Chemical data obtained from different fumaroles collected at Vulcano Island (Sicily, southern Italy) between 2000 and 2004 have been analysed statistically. The methodology has identified parameters able to elucidate the structure of the complex fumarolic field of Vulcano in the investigated span of time, notwithstanding the high data variability. The southern portion of the Tyrrhenian Sea was affected by an earthquake (M = 5.8, 40 km NE of Palermo) in December 2002. Abrupt outgassing on the island of Panarea occurred in November 2002 and Stromboli was significantly active from December 2002 to July 2003. The great geological instability of the area is thought to have had an influence on the variability shown by the data. The time-dependent variations in the components of the data have been investigated using logratios. The H2O-HCl-SO2 subcomposition, for a limited set of fumaroles, has been used to check for log-contrast principal components to be considered as a monitoring tool of volcanic activity. Results obtained indicate that the compositional changes are a complex function of time, chemistry, temperature and space. ANOVA (analysis of variance) of log-ratios for which there is no time dependence has elucidated components subject to significant spatial variations across the fumarole field, due to changes in redox conditions, and components dominated by random variations.
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