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  • 1
    Electronic Resource
    Electronic Resource
    Isozyme gene markers in the dioecious species Asparagus officinalis L. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 613-618 
    Details
    ISSN: 1432-2242
    Keywords: Asparagus officinalis ; Isoenzymes ; Marker genes ; Sex-linked inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Extracts from phylloclads of Asparagus officinails were electrophoretically analyzed for isozyme polymorphism. Fourteen enzyme systems were examined using four buffer systems: seven enzymes (acid phosphatase, catalase, glutamate-oxaloacetate transaminase, isocitrate dehydrogenase, malate dehydrogenase, peroxidase, and 6-phosphogluconate dehydrogenase) exhibited clear and consistent banding patterns. Isozyme polymorphism was studied in seven pairs of male and female doubled haploids and in their male F1s. Segregation of polymorphic loci was examined in the backcross progenies and was found to be consistent with a simple Mendelian inheritance in all cases, except for three anodical peroxidases, where two factors have been hypothesized. No linkage could be found between isozyme markers that were segregating in the same cross, but association was demonstrated between one malate dehydrogenase locus and the sex determining genes. The availability of isozyme markers may be useful in breeding and, in particular, the localization of one malate dehydrogenase locus on the sex chromosomes may be helpful in mapping the sex genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226726
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  • 2
    Electronic Resource
    Electronic Resource
    Development of male and female flower in Asparagus officinalis. Search for point of transition from hermaphroditic to unisexual developmental pathway (1994)
    Springer
    Sexual plant reproduction 7 (1994), S. 239-249 
    Details
    ISSN: 1432-2145
    Keywords: Asparagus ; Flower development ; Sex differentiation ; Male and female RNase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Asparagus officinalis is a dioecious plant. The flowers start to develop as hermaphrodites and later become unisexual. In female flowers the stamens degenerate, while in male flowers the ovary stops growing without degenerating. We have examined young asparagus flowers using SEM and optical microscopy in order to determine the exact moment of transition from hermaphroditic to unisexual development. We defined 13 stages of development, starting from flower primordia up to completely mature flowers and labelled them with numbers from -6 to 7. The first five stages are fully hermaphroditic: a difference between sexes becomes visible at stage — 1 when the style begins to develop in female flowers. Degeneration of stamens in female flowers starts somewhat later. At the stage of transition, some differences between sexes also appear in the bidimensional polypeptide pattern of flowers. RNase activity shows a distinct peak at this stage (in female flowers only), probably related to stamen degeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232743
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    Paper (German National Licenses)
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