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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 66 (1992), S. 235-241 
    ISSN: 1573-5060
    Keywords: cryopreservation ; English roses ; liquid nitrogen storage ; pollen ; Rosa spp. ; storage ; viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An investigation was undertaken on the storage characteristics of pollen collected from two English rose cultivars. A rapid decline in viability was observed in pollen stored at +4° C and −20° C, whereas the viability of pollen, stored at ultra-low temperature (−196° C), remained constant. Cryopreserved pollen was shown to retain its ability for fertilisation. The effects of the stage of flower development and anther dehiscence were assessed on both pre-and post-cryopreservation viabilities. Successful long-term storage of pollen will facilitate hybridisation of rose species and cultivars that do not flower synchronously.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant growth regulation 20 (1996), S. 43-45 
    ISSN: 1573-5087
    Keywords: cryopreservation ; encapsulation/dehydration ; rose ; Rosa multiflora ; shoot tips
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Shoot tips of in vitro grown plantlets of Rosa multiflora were cryopreserved using an encapsulation/dehydration procedure. The influence of sucrose and silica gel pretreatments on pre- and post-freeze shoot growth were examined. Shoot tips recovered from liquid nitrogen only grew after 24h pretreatment in medium containing 0.5 M sucrose, followed by 2 h drying with silica gel and rapid freezing.
    Type of Medium: Electronic Resource
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