Summary
An investigation was undertaken on the storage characteristics of pollen collected from two English rose cultivars. A rapid decline in viability was observed in pollen stored at +4° C and −20° C, whereas the viability of pollen, stored at ultra-low temperature (−196° C), remained constant. Cryopreserved pollen was shown to retain its ability for fertilisation. The effects of the stage of flower development and anther dehiscence were assessed on both pre-and post-cryopreservation viabilities. Successful long-term storage of pollen will facilitate hybridisation of rose species and cultivars that do not flower synchronously.
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Marchant, R., Power, J.B., Davey, M.R. et al. Cryopreservation of pollen from two rose cultivars. Euphytica 66, 235–241 (1992). https://doi.org/10.1007/BF00025309
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DOI: https://doi.org/10.1007/BF00025309