ISSN:
1573-0603
Keywords:
End-labeling
;
GBS
;
Mapping
;
Restriction enzyme mapping
;
Tn917
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two pieces of data are needed to fully map the location of a transposon inserted in a plasmid, the site of insertion and the transposon's orientation. Both of these parameters can be determined from a map of restriction sites, which can be derived by end-probing. Like restriction mapping, end-probing reveals the distance between restriction sites on a plasmid. In contrast to restriction mapping, end-probing unambiguously reveals the order of those restriction sites. End-probing is similar to end-labeling, except that the uncertainties inherent in the radiolabeling reaction (and the problems of working with radionucleotides) are avoided. In this paper we discuss the use of end-probing as a means to map insertions of transposon Tn917 into the Streptococcus agalactiae plasmid pGB354
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009851211362
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