Abstract
Two pieces of data are needed to fully map the location of a transposon inserted in a plasmid, the site of insertion and the transposon's orientation. Both of these parameters can be determined from a map of restriction sites, which can be derived by end-probing. Like restriction mapping, end-probing reveals the distance between restriction sites on a plasmid. In contrast to restriction mapping, end-probing unambiguously reveals the order of those restriction sites. End-probing is similar to end-labeling, except that the uncertainties inherent in the radiolabeling reaction (and the problems of working with radionucleotides) are avoided. In this paper we discuss the use of end-probing as a means to map insertions of transposon Tn917 into the Streptococcus agalactiae plasmid pGB354
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Lee, M.H., Nittayajarn, A. & Rubens, C.E. End-probing: A non-radioactive approach to mapping transposon insertions. Methods Cell Sci 20, 113–118 (1998). https://doi.org/10.1023/A:1009851211362
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DOI: https://doi.org/10.1023/A:1009851211362