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  • 1
    ISSN: 1570-7458
    Keywords: Spodoptera littoralis ; pesticides ; RH-5849 ; ecdysteroids ; pupation ; circadian rhythmicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Under laboratory conditions (L16:D8, 24°C, r.h. 80%), applying of an ecdysone agonist, RH 5849, (1,2-dibenzoyl-1-tert-butylhydrazine) onto final instar larvae of Spodoptera littoralis (Boisd.), Noctuidae, Lepidoptera, delayed or advanced the time of pupation depending on the time of treatment. This response was dose-dependent. In addition, non-lethal doses of RH 5849, ranging from 0.05 to 10 µg/larva were used. Following treatment of S. littoralis larvae with 5 µg RH 5849 the endogenous ecdysteroid level was increased or decreased depending on the time of application. Based on the results presented, we suggest that potential users of non-steroidal ecdysone agonists should consider the proper circadian timing of application in the field.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1570-7458
    Keywords: nonsteroidal ecdysone mimic ; RH-0345 ; tebufenozide ; RH-5849 ; precocious moulting ; polypeptide synthesis ; haemolymph ; cuticle ; ultrastructure ; Leptinotarsa decemlineata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Larvae of the Colorado potato beetle, Leptinotarsa decemlineata (Say), that were orally treated with RH-0345 at 0.1 mg l−1, RH-5849 at 10 and 50 mg l−1, tebufenozide at 2 g l−1, and 20-hydroxyecdysone at 2 g l−1, showed symptoms of premature moulting, followed by inhibition of ecdysis. In addition, fresh weight gain and total protein content were blocked. The effects on haemolymphal and cuticular polypeptides after PAGE were linked with premature, new epicuticle deposition as was observed under the electron microscope. These observations support the concept that the ecdysteroid-mimicking action of the three nonsteroidal molecules is specific.
    Type of Medium: Electronic Resource
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