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  • 1
    Digitale Medien
    Digitale Medien
    Endocrine background of how 20-hydroxyecdysone agonist, RH 5849, influences diurnal pattern of pupation in shape Spodoptera littoralis (1998)
    Springer
    Entomologia experimentalis et applicata 87 (1998), S. 255-261 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Spodoptera littoralis ; pesticides ; RH-5849 ; ecdysteroids ; pupation ; circadian rhythmicity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Under laboratory conditions (L16:D8, 24°C, r.h. 80%), applying of an ecdysone agonist, RH 5849, (1,2-dibenzoyl-1-tert-butylhydrazine) onto final instar larvae of Spodoptera littoralis (Boisd.), Noctuidae, Lepidoptera, delayed or advanced the time of pupation depending on the time of treatment. This response was dose-dependent. In addition, non-lethal doses of RH 5849, ranging from 0.05 to 10 µg/larva were used. Following treatment of S. littoralis larvae with 5 µg RH 5849 the endogenous ecdysteroid level was increased or decreased depending on the time of application. Based on the results presented, we suggest that potential users of non-steroidal ecdysone agonists should consider the proper circadian timing of application in the field.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1003258319630
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Effects of the ecdysteroid agonists RH 5849 an RH 5992, alone and in combination with a juvenile hormone analogue, pyriproxyfen, on larvae ofSpodoptera exigua (1994)
    Springer
    Entomologia experimentalis et applicata 72 (1994), S. 115-123 
    Details
    ISSN: 1570-7458
    Schlagwort(e): ecdysteroid agonists ; juvenile hormone analogue ; Spodoptera exigua ; growth ; moulting ; metamorphosis ; imaginal discs
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Biological activity assays with RH 5849 and RH 5992 indicated that both compounds affected growth and development of last-instar larvae ofSpodoptera exigua (Hübner) (Lepidoptera: Noctuidae) in a dose-dependent manner. Within the first 24 h after treatment by continuously offering leaves dipped in a water solution of ≥50 mg/l RH 5849 and ≥0.5 mg/l RH 5992, symptoms of a prematurely induced larval moult and head capsule apolysis were visible. Intoxicated larvae died shortly afterwards, showing signs of unsuccessful ecdysis. LC50-values of RH 5849 and RH 5992 for fifth-instarS. exigua larvae were 110 and 2.5 mg/l, respectively. Pyriproxyfen alone affected the larval stage and disturbed normal metamorphosis. One supernumerary larval instar occurred occasionally. LC50-value for pyriproxyfen was 1.7 mg/l. Larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, continued to grow until they attained a size and weight about 2–3 times that of the controls. This growth was accompanied by at least one and sometimes two supernumerary moults. Concerning thein vivo imaginal wing disc growth and development, only in larvae treated with 10 and 50 mg/l RH 5849 or 0.5 mg/l RH 5992, tracheole migration was observed earlier than in the controls. When applying 300 mg/l RH 5849 or 3–7 mg/l RH 5992, the discs remained small and no signs of tracheole migration were observed. In larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, tracheole migration was not prematurely induced and a pupal cuticle was produced in the discs of larvae, undergoing a supernumerary moult. No clear signs of evagination were observed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02383546
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Significance of penetration and metabolism on topical toxicity of diflubenzuron in Spodoptera littoralis and Spodoptera exigua (1997)
    Springer
    Entomologia experimentalis et applicata 82 (1997), S. 255-260 
    Details
    ISSN: 1570-7458
    Schlagwort(e): diflubenzuron ; topical toxicity ; cuticle penetration ; metabolism ; Spodoptera littoralis/ ; Spodoptera exigua
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Toxicity assays indicated that when diflubenzuron (DFB) was topically applied on larvae of the Egyptian cotton leafworm, Spodoptera littoralis (Boisduval), and the beet armyworm, Spodoptera exigua (Hübner), estimated LD50 values were similar, reaching 0.47 and 0.44 ng/larva, respectively. This study evaluated the importance of the rate of uptake and excretion, and of enzymatic metabolism in building up an insecticidal toxicity after topical application on the insect cuticle in both species. In general, penetration of DFB in S. littoralis was about 2-fold higher than in S. exigua, whereas metabolic breakdown was of minor importance in S. littoralis as compared with S. exigua. The current data indicate the importance of cuticle penetration and discuss that extrapolation from oral feeding toxicity results to topical toxicity data should be evaluated with caution, especially for insect growth regulators used in integrated pest management.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1002948719578
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Nonsteroidal moulting hormone agonists: effects on protein synthesis and cuticle formation in Colorado potato beetle larvae (1999)
    Springer
    Entomologia experimentalis et applicata 93 (1999), S. 1-8 
    Details
    ISSN: 1570-7458
    Schlagwort(e): nonsteroidal ecdysone mimic ; RH-0345 ; tebufenozide ; RH-5849 ; precocious moulting ; polypeptide synthesis ; haemolymph ; cuticle ; ultrastructure ; Leptinotarsa decemlineata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Larvae of the Colorado potato beetle, Leptinotarsa decemlineata (Say), that were orally treated with RH-0345 at 0.1 mg l−1, RH-5849 at 10 and 50 mg l−1, tebufenozide at 2 g l−1, and 20-hydroxyecdysone at 2 g l−1, showed symptoms of premature moulting, followed by inhibition of ecdysis. In addition, fresh weight gain and total protein content were blocked. The effects on haemolymphal and cuticular polypeptides after PAGE were linked with premature, new epicuticle deposition as was observed under the electron microscope. These observations support the concept that the ecdysteroid-mimicking action of the three nonsteroidal molecules is specific.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1003848728677
    Standort Signatur Erwartet Verfügbarkeit
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