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  • 1
    Electronic Resource
    Electronic Resource
    Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division, in protoplasts of Nicotiana tabacum L. cultivated in vitro (1978)
    Springer
    Planta 142 (1978), S. 253-262 
    Details
    ISSN: 1432-2048
    Keywords: Cell wall formation ; Cytokinesis ; Inhibitors ; Mitosis ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of cytochalasin B, colchicine, coumarin and 2,6-dichlorobenzonitrile on cell wall formation and cellular division was studied by light and electron microscopy with tobacco mesophyll protoplasts cultivated in vitro. 2,6-dichlorobenzonitrile was found to be the most effective and reversible inhibitor of cell wall formation. The other inhibitors caused irreversible damage and/or inhibited mitosis. In protoplasts cultivated in the presence of 2,6-dichlorobenzonitrile the total inhibition of cell wall formation had no effect on nuclear division, but cytokinesis was totally inhibited so that multinucleate protoplasts were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385074
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  • 2
    Electronic Resource
    Electronic Resource
    Time course of hormonal control of the first mitosis in tobacco mesophyll protoplasts cultivated in vitro (1979)
    Springer
    Planta 147 (1979), S. 181-185 
    Details
    ISSN: 1432-2048
    Keywords: Mitosis ; Nicotiana ; Phytohormones ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The presence of auxin and cytokinin is necessary for the induction of mitosis in tobacco mesophyll protoplasts cultivated in vitro. In their absence, protoplasts firstly accumulate inhibitors of mitosis in the culture medium, possibly because of non-coordinated cell-wall synthesis, and secondly evolve a nonmitotic and degenerative metabolism. By changing the intoxified medium, it is possible to show that auxin is necessary from the beginning of culture, while cytokinin is only required later to allow a step in the development of the mitotic apparatus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388735
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    Paper (German National Licenses)
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