Abstract
The effect of cytochalasin B, colchicine, coumarin and 2,6-dichlorobenzonitrile on cell wall formation and cellular division was studied by light and electron microscopy with tobacco mesophyll protoplasts cultivated in vitro. 2,6-dichlorobenzonitrile was found to be the most effective and reversible inhibitor of cell wall formation. The other inhibitors caused irreversible damage and/or inhibited mitosis. In protoplasts cultivated in the presence of 2,6-dichlorobenzonitrile the total inhibition of cell wall formation had no effect on nuclear division, but cytokinesis was totally inhibited so that multinucleate protoplasts were obtained.
Similar content being viewed by others
Abbreviations
- DB:
-
2,6-dichlorobenzonitrile=“dichlobenil”
- CB:
-
cytochalasin B
References
Burgess, J., Linstead, P.J.: Coumarin inhibition of microfibril formation at the surface of cultured protoplasts. Plants 133, 267–273 (1977)
Capesius, I., Meyer, Y.: Isolation of nuclei from protoplasts of orchids. Cytobiol. 15, 485–490 (1977)
Chrispeels, M.J.: Biosynthesis, intracellular transport, and secretion of extracellular macromolecules. Ann. Rev. Plant Physiol. 27, 19–38 (1976)
Cocking, E.C.: Plant cell protoplasts — isolation and development. Ann. Rev. Plant Physiol. 23, 29–50 (1972)
Franke, W.W., Morré, J., Herth, W., Zerban, H.: I. Cytology a) General and molecular cytology. 1. Structure of the Golgi apparatus and association with other cell components. Fortschr. Bot. 38, 1–16 (1976)
Frearson, E.M., Power J.B., Cocking, E.C.: The isolation, culture, and regeneration of Petunia leaf protoplasts. Dev. Biol. 33, 130–137 (1973)
Harada, H., Ohyama, K., Cheruel, J.: Effects of coumarin and other factors on the modification of form and growth of isolated mesophyll cells. Z. Pflanzenphysiol. 66, 307–324 (1972)
Herth, W., Meyer, Y.: Ultrastructural and chemical analysis of the wall fibrils synthesized by tobacco mesophyll protoplasts. Biol. Cellulaire 30, 33–40 (1977)
Herth, W., Meyer, Y.: Cytology of budding and cleavage in tobacco mesophyll protoplasts cultivated in saline medium. Planta in press
Herth, W., Franke, W.W., VanDerWoude, W.J.: Cytochalasin stops tip growth in plants. Naturwiss. 59, 38–39 (1972)
Hepler, P.K., Palevitz, B.A.: Microtubules and microfilaments. Ann. Rev. Plant Physiol. 25 309–362 (1974)
Hogetsu, T., Shibaoka, H., Shimokoriyama, M.: Involvement of cellulose synthesis in actions of gibberellin and kinetin on cell expansion. 2,6-dichlorobenzonitrile as a new cellulose-synthesis inhibitor. Plant Cell Physiol. 15, 389–393 (1974)
Meyer, Y.: Isolation and culture of tobacco mesophyll protoplasts using a saline medium. Protoplasma 81, 363–372 (1974)
Meyer, Y., Abel, W.O.: Importance of the wall for cell division and in the activity of the cytoplasm in cultured tobacco protoplasts. Planta 123, 33–40 (1975a)
Meyer, Y., Abel, W.O.: Budding and cleavage division of tobacco mesophyll protoplasts in relation to pseudo-wall and wall formation. Planta 125, 1–13 (1975b)
Mollenhauer, H.H., Morré, D.J.: Cytochalasin B, but not colchicine, inhibits migration of secretory vesicles in root tips of maize. Protoplasma 87, 39–48 (1976)
Nagata, T., Takebe, I.: Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta 92, 301–308 (1970)
O'Brien, T.P.: The cytology of cell-wall formation in some eucaryotic cells. Bor. Rev. 38, 87–118 (1972)
Pearce, R.S., Cocking, E.C., Behaviour in culture of isolated protoplasts from “Paul's Scarlet” rose suspension culture cells. Protoplasma 77, 165–180 (1973)
Robenek, H., Peveling, E.: Ultrastructure of the cell wall regeneration of isolated protoplasts of Skimmia japonica Thunb. Planta 136, 135–145 (1977)
Satoh, S., Matsuda, K., Tamari, K.: β-1,4-glucan occurring in homogenate of Phaseolus aureus seedlings. Possible nascent stage of cellulose biosynthesis in vivo. Plant Cell Physiol. 17, 1243–1254 (1976a)
Satoh, S., Takahara, M., Matsuda, K.: Inhibition of the synthesis of microbial cellulose by coumarin. Plant Cell Physiol. 17, 1077–1080 (1976b)
Sawhney, V.K., Srivastava, L.M.: Comparative effects of cytochalasin B and colchicine on lettuce seedlings. Ann. Bot. 41, 271–274 (1977)
Schilde-Rentschler, L.: Role of the cell wall in the ability of tobacco protoplasts to form callus. Planta 135, 177–181 (1977)
Schnepf, E.: Microtubules and cell wall formation. Portugal. Acta Biol. Ser. A 14, 451–462 (1974)
Schnepf, E., Röderer, G., Herth, W.: The formation of the fibrils in the lorica of Poteriochromonas stipitata. Tip growth, kinetics, site, orientation. Planta 125, 42–62 (1975)
Srivastava, L.M., Sawhney, V.K., Bonettemaker, M.: Cell growth, wall deposition, and correlated fine structure of colchicinetreated lettuce hypocotyl cells. Can. J. Bot. 55, 902–917 (1977)
Umetsu, N., Satoh, S., Matsuda, K.: Effects of 2,6-dichlorobenzonitrile on suspension-cultured soybean cells. Plant Cell Physiol. 17, 1071–1075 (1975)
Vannini, G.L., Fasulo, M.P., Bruni, A.: First observations on the cytological changes induced in Euglena gracilis by coumarin treatment. Z. Pflanzenphysiol. 84, 183–187 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Meyer, Y., Herth, W. Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division, in protoplasts of Nicotiana tabacum L. cultivated in vitro. Planta 142, 253–262 (1978). https://doi.org/10.1007/BF00385074
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00385074