Abstract
The effect of cytochalasin B, colchicine, coumarin and 2,6-dichlorobenzonitrile on cell wall formation and cellular division was studied by light and electron microscopy with tobacco mesophyll protoplasts cultivated in vitro. 2,6-dichlorobenzonitrile was found to be the most effective and reversible inhibitor of cell wall formation. The other inhibitors caused irreversible damage and/or inhibited mitosis. In protoplasts cultivated in the presence of 2,6-dichlorobenzonitrile the total inhibition of cell wall formation had no effect on nuclear division, but cytokinesis was totally inhibited so that multinucleate protoplasts were obtained.
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Abbreviations
- DB:
-
2,6-dichlorobenzonitrile=“dichlobenil”
- CB:
-
cytochalasin B
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Meyer, Y., Herth, W. Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division, in protoplasts of Nicotiana tabacum L. cultivated in vitro. Planta 142, 253–262 (1978). https://doi.org/10.1007/BF00385074
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DOI: https://doi.org/10.1007/BF00385074