Publikationsdatum:
1996-12-20
Beschreibung:
Adipocyte differentiation is an important component of obesity and other metabolic diseases. This process is strongly inhibited by many mitogens and oncogenes. Several growth factors that inhibit fat cell differentiation caused mitogen-activated protein (MAP) kinase-mediated phosphorylation of the dominant adipogenic transcription factor peroxisome proliferator-activated receptor gamma (PPARgamma) and reduction of its transcriptional activity. Expression of PPARgamma with a nonphosphorylatable mutation at this site (serine-112) yielded cells with increased sensitivity to ligand-induced adipogenesis and resistance to inhibition of differentiation by mitogens. These results indicate that covalent modification of PPARgamma by serum and growth factors is a major regulator of the balance between cell growth and differentiation in the adipose cell lineage.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hu, E -- Kim, J B -- Sarraf, P -- Spiegelman, B M -- R37DK31405/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1996 Dec 20;274(5295):2100-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Dana-Farber Cancer Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8953045" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
3T3 Cells
;
Adipocytes/*cytology/metabolism
;
Animals
;
Blood
;
Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
;
Cell Differentiation
;
Cell Line
;
Enzyme Inhibitors/pharmacology
;
Epidermal Growth Factor/pharmacology
;
Flavonoids/pharmacology
;
Insulin/pharmacology
;
Ligands
;
Mice
;
Mitogens/pharmacology
;
Mutation
;
Phosphorylation
;
Rats
;
Receptors, Cytoplasmic and Nuclear/chemistry/genetics/*metabolism
;
Tetradecanoylphorbol Acetate/pharmacology
;
Transcription Factors/chemistry/genetics/*metabolism
;
Transcription, Genetic/drug effects
;
Transfection
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
Permalink
