ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Mapping of the Hor-3 locus encoding D hordein in Barley (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 367-371 
    Details
    ISSN: 1432-2242
    Keywords: Storage proteins ; Hordein D ; Three point test ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The hordein storage proteins of barley (Hordeum vulgare L.) are of intense interest due to their genetic diversity and prominence and impact on the industrial and agricultural uses of the seed. Two major hordein loci have been previously mapped on chromosome 5 (Hor-1 and Hor-2 encoding the C and B hordeins, respectively). A third major locus, Hor-3, which codes for D hordein, has been located in the centromeric region of chromosome 5, probably on the long arm. Two allelic variants with apparent molecular weights of 83,000 and 91,000 and similar isoelectric points of 8.0 comprise the products of this locus in the barley varieties ‘Advance’ and ‘Triple Awned Lemma’. The D hordein examined is similar in molecular weight and isoelectric point to the high molecular weight (HMW) glutenin proteins encoded by the 1B chromosome of wheat (Triticum aestivum L.)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303909
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Use of the additive main effects and multiplicative interaction model in QTL mapping for adaptation in barley (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 30-37 
    Details
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Quantitative trait loci ; Genotype x environment interaction ; Pattern analysis ; Molecular marker-assisted selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The additive main effects and multiplicative interaction (AMMI) model has emerged as a powerful analytical tool for genotype x environment studies. The objective of the present study was to assess its value in quantitative trait locus (QTL) mapping. This was done through the analysis of a large two-way table of genotype-by-environment data of barley (Hordeum vulgare L.) grain yields, where the genotypes constituted a genetic population suitable for mapping studies. Grain yield data of 150 doubled haploid lines derived from the ‘Steptoe’ x ‘Morex’ cross, and the two parental lines, were taken by the North American Barley Genome Mapping Project (NABGMP) at 16 environments throughout the barley production areas of the USA and Canada. Four regions of the genome were responsible for most of the differential genotypic expression across environments. They accounted for approximately 50% of the genotypic main effect and 30% of the genotype x environment interaction (GE) sums of squares. The magnitude and sign of AMMI scores for genotypes and sites facilitate inferences about specific interactions. The parallel use of classification (cluster analysis of environments) and ordination (principal component analysis of GE matrix) techniques allowed most of the variation present in the genotype x environment matrix to be summarized in just a few dimensions, specifically four QTLs showing differential adaptation to four clusters of environments. Thus, AMMI genotypic scores, when the genotypes constituted a population suitable for QTL mapping, could provide an adequate way of resolving the magnitude and nature of QTL x environment interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225723
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Verification of barley seed dormancy loci via linked molecular markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 87-91 
    Details
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Dormancy ; Quantitative trait loci ; Molecular marker-assisted selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seed dormancy is a relatively complex trait in barley (Hordeum vulgare L.). Several dormancy loci were identified previously by quantitative trait locus analysis. Three reciprocal crosses were made in the present study between parents carrying specific dormancy alleles via linked molecular markers to verify individual dormancy locus effects and potential expression. Analyses of F2 progenies revealed that the dormancy allele at the locus flanked by the markers Ale and ABC302 on the long arm of chromosome 7 had a major effect on dormancy, and was at least partly epistatic to the dormancy locus in the ABC309-MWG851 interval near the telomere of the long arm of chromosome 7. In the absence of the dormancy allele in the Ale-ABC302 interval, the allele in the ABC309-MWG851 interval exerted moderate to large effects on dormancy. Cytoplasmic effects on dormancy were also observed. The germination percentages of progeny with relatively high levels of dormancy were more variable than those of non-dormant or less-dormant progeny, apparently due to environmental effects. Removal of the dormancy allele in the Ale-ABC302 interval, or introducing the dormancy allele in the ABC309-MWG851 interval, should suffice for adjusting dormancy levels in breeding programs to suit various production situations and end uses. The verification of dormancy loci via linked molecular markers allows manipulation of these loci in applied breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222956
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Mapping of β-glucan content and β-glucanase activity loci in barley grain and malt (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 921-927 
    Details
    ISSN: 1432-2242
    Keywords: QTL mapping ; β-Glucan ; β-Glucanase Malt barley ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic study of β-glucan content and β-glucanase activity has been facilitated by recent developments in quantitative trait loci (QTL) analysis. QTL for barley and malt β-glucan content and for green and finished malt β-glucanase activity were mapped using a 123-point molecular marker linkage map from the cross of Steptoe/Morex. Three QTL for barley β-glucan, 6 QTL for malt β-glucan, 3 QTL for β-glucanase in green malt and 5 QTL for β-glucanase in finished malt were detected by interval mapping procedures. The QTL with the largest effects on barley β-glucan, malt βglucan, green malt β-glucanase and finished malt βglucanase were identified on chromosomes 2,1,4 and 7, respectively. A genome map-based approach allows for dissection of relationships among barley and malt βglucan content, green and finished malt β-glucanase activity, and other malting quality parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223901
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Barley tissue as direct template for PCR: a practical breeding tool (1996)
    Springer
    Molecular breeding 2 (1996), S. 181-183 
    Details
    ISSN: 1572-9788
    Keywords: barley ; breeding ; Hordeum vulgare ; polymerase chain reaction ; tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A method for using alkali treated intact plant tissue as a DNA source for the polymerase chain reaction (PCR) was applied to barley. This method saves up to two days and more than USD 50 per 40 samples by eliminating the need for DNA extraction to produce template for PCR. The conditions were optimized for various barley tissues. Fresh leaves, freeze-dried leaves, and anthers worked well as templates while root, embryo, and endosperm tissues did not. The method was shown to work with several genotypes and different primers. The resulting PCR product could be cut with restriction enzyme to produce clear polymorphism without any interference. This method can be a practical breeding tool by providing a fast, inexpensive method for screening large populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00441433
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Verification of yield QTL through realized molecular marker- assisted selection responses in a barley cross (1999)
    Springer
    Molecular breeding 5 (1999), S. 143-152 
    Details
    ISSN: 1572-9788
    Keywords: Hordeum vulgare ; molecular marker-assisted selection ; quantitative trait loci ; genotype × environment interaction ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Verification of putative quantitative trait loci (QTL) is an essential step towards implementing the use of marker-assisted selection (MAS) in cultivar improvement. In a previous study with 150 doubled haploid lines derived from the 6-row cross Steptoe/Morex (S/M), four regions (QTL1–4) of the barley genome were associated with differential genotypic expression for grain yield across environments. The objectives of this study were to verify the value of these four QTL for selection and to compare the efficiency of alternative MAS strategies using these QTL vs. conventional phenotypic selection for grain yield. A total of 92 DHLs derived from the S/M cross that were not used in the original mapping efforts were used for QTL verification. Confirmation of QTL effects was first accomplished by assessing yield differences between individuals carrying alternative alleles at each putative locus in three environments. QTL1 on chromosome 3 was confirmed as the most important and consistent locus to determine yield across sites, with the S allele being favorable. The M allele at QTL3 on chromosome 6 was beneficial for grain yield across sites, but to a lesser degree than QTL1. Magnitudes of allele effects at QTL2 (chromosome 2) and QTL4 (chromosome 7) were highly influenced by the environment where the genotypes were grown. Verification of QTL effects was best achieved by comparing realized selection response. Genotypic (MAS) and tandem genotypic and phenotypic selection were at least as good as phenotypic selection. Consistent selection responses were detected for QTL1 alone and together with QTL3. Genotypic selection for lines carrying the S allele at QTL1 resulted in the identification of high-yielding genotypes. Selection responses increased when the M allele at QTL3 was combined with the S allele at QTL1. Significant qualitative QTL × environment interactions for QTL2 and QTL4 were detected through differential realized selection responses at different sites. Without a thorough understanding of the physiological and agronomic particulars of any QTL and the target environment, MAS for QTL showing qualitative interactions should be minimized
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009684108922
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Molecular marker-assisted selection for malting quality traits in barley (1997)
    Springer
    Molecular breeding 3 (1997), S. 427-437 
    Details
    ISSN: 1572-9788
    Keywords: Hordeum vulgare ; malting quality ; molecular marker-assisted selection ; quantitative trait loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Selection for malting quality in breeding programs by micromalting and micromashing is time-consuming, and resource-intensive. More efficient and feasible approaches for identifying genotypes with good malting quality would be highly desirable. With the advent of molecular markers, it is possible to map and tag the loci affecting malting quality. The objective of this study was to assess the effectiveness of molecular marker assisted selection for malting quality traits. Two major quantitative trait loci (QTL) regions in six-row barley for malt extract percentage, α-amylase activity, diastatic power, and malt β-glucan content on chromosomes 1 (QTL1) and 4 (QTL2) have been previously identified. The flanking markers, Brz and Amy2, and WG622 and BCD402B, for these two major QTL regions were used in marker-assisted selection. Four alternative selection strategies; phenotypic selection, genotypic selection, tandem genotypic and phenotypic selection, and combined phenotypic and genotypic selection, were compared for both single and multiple trait selection in a population consisting of 92 doubled haploid lines derived from ‘Steptoe’ × ‘Morex’ crosses. Marker assisted selection for QTL1 (tandem genotypic and phenotypic selection, and combined phenotypic and genotypic selection) was more effective than phenotypic selection, but for QTL2 was not as effective as phenotypic selection due to a lack of QTL2 effects in the selection population. The effectiveness of tandem genotypic and phenotypic selection makes marker assisted selection practical for traits which are extremely difficult or expensive to measure such as most malting quality traits. It can substantially eliminate undesirable genotypes by early genotyping and keeping only desirable genotypes for later phenotypic selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009608312385
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...