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  • Gene library  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Cloning and characterization of the recA gene of Aquaspirillum magnetotacticum (1989)
    Springer
    Archives of microbiology 152 (1989), S. 567-571 
    Details
    ISSN: 1432-072X
    Keywords: RecA gene ; Aquaspirillum magnetotacticum ; Gene library ; Recombinant cosmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The recA gene of Aquaspirillum magnetotacticum has been isolated from a genomic library and introduced into a recA mutant strain of Escherichia coli K12. The cloned gene complemented both the recombination and DNA repair deficiency of the host and its protein product promoted the proteolytic cleavage of the LexA protein. A protein whose molecular weight is similar to that of the RecA protein of E. coli was associated with the cloned sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425488
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  • 2
    Electronic Resource
    Electronic Resource
    Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12 (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 592-594 
    Details
    ISSN: 1617-4623
    Keywords: Gene library ; Recombinant cosmid ; Aquaspirillum magnetotacticum ; magnetotaxis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gene libraries from the magnetotactic bacterium, Aquaspirillum magnetotacticum were constructed in Escherichia coli with cosmids pLAFR3 and c2RB as vectors. Recombinant cosmids able to complement the thr-1, leuB, and proA mutations of the host were identified. The Pro+ recombinant cosmid restored wild-type phenotype in proA and proB but not in the proC mutants of E. coli. The results of restriction endonuclease digestion and Southern hybridization analysis indicate that the relevent leu and pro biosynthetic genes of A. magnetotacticum are not closely linked on the chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330500
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    Paper (German National Licenses)
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