Abstract
The recA gene of Aquaspirillum magnetotacticum has been isolated from a genomic library and introduced into a recA mutant strain of Escherichia coli K12. The cloned gene complemented both the recombination and DNA repair deficiency of the host and its protein product promoted the proteolytic cleavage of the LexA protein. A protein whose molecular weight is similar to that of the RecA protein of E. coli was associated with the cloned sequence.
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Bates PF, Swift RA (1983) Double cos site vectors: simplified cosmid cloning. Gene 26:137–146
Better M, Helinski DR (1983) Isolation and characterization of the recA gene of Rhizobium meliloti. J Bacteriol 155:311–316
Blakemore RP (1982) Magnetotactic bacteria. Ann Rev Microbiol 36:217–238
Blakemore RP, Maratea D, Wolfe RS (1979) Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium. J Bacteriol 140:720–729
Clark AJ (1973) Recombination-deficient mutants of E. coli and other bacteria. Ann Rev Genet 7:67–86
Goldberg I, Mekalanos JJ (1986) Cloning of the Vibrio cholerae recA gene and construction of a Vibrio cholerae mutant. J Bacteriol 165:715–722
Hanawalt PC, Cooper PK, Ganesan AK, Smith CA (1979) DNA repair in bacteria and mammalian cells. Ann Rev Biochem 48:60–93
Keener SL, McNamee KP, McEntee K (1984) Cloning and characterization of recA genes from Proteus vulgaris, Erwinia carotovora, Shigella flexneri, and Escherichia coli B/r. J Bacteriol 160:153–160
Koomey JM, Falkow S (1987) Cloning of the recA gene of Neisseria gonorrhoeae and construction of genococcal recA mutants. J Bacteriol 169:790–795
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratories, Cold Spring Harbor, NY
Manly KF, Singer ER, Radding CM (1969) Nonessential functions of bacteriophage λ. Virology 37:177–188
Mount DW (1977) A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways. Proc Natl. Acad Sci USA 74:300–304
Ohman DE, West MA, Flynn JL, Goldberg JB (1985) Methods for gene replacement in Pseudomonas aeruginosa used in construction of recA mutants: recA-independent instability of alginate production. J Bacteriol 162:1068–1074
Ozeki H, Stocker BAD, Smith SM (1962) Transmission of colicinogeny between strains of Salmonella typhimurium grown together. J Gen Microbiol 28:671–687
Pierre A, Paoletti C (1983) Purification and characterization of RecA protein from Salmonella typhimurium. J Bacteriol 258:2870–2874
Rodriguez RL, Tait RC (1983) Recombinant DNA techniques: An introduction. Addison-Wesley, Reading, MASS
Sancar A, Stachelek C, Koningsberg W, Rupp WD (1980) Sequences of recA gene and protein. Proc Natl Acad Sci USA 77:2611–2615
Waleh NS (1988) Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli. Mol Gen Genet 214:592–594
Waleh NS, Johnson PJ (1985) Structural and functional characterization of the colicin E1 operon. Proc Natl Acad Sci USA 82:8389–8393
Walker GC (1984) Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev 48:60–93
West SC, Countryman JK, Howard-Flanders P (1983) Purification and properties of the recA protein of Proteus mirabilis. J Biol Chem 258:4648–4654
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This paper is affectionately dedicated to Prof. John L. Ingraham
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Berson, A.E., Hudson, D.V. & Waleh, N.S. Cloning and characterization of the recA gene of Aquaspirillum magnetotacticum . Arch. Microbiol. 152, 567–571 (1989). https://doi.org/10.1007/BF00425488
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DOI: https://doi.org/10.1007/BF00425488