Summary
Gene libraries from the magnetotactic bacterium, Aquaspirillum magnetotacticum were constructed in Escherichia coli with cosmids pLAFR3 and c2RB as vectors. Recombinant cosmids able to complement the thr-1, leuB, and proA mutations of the host were identified. The Pro+ recombinant cosmid restored wild-type phenotype in proA and proB but not in the proC mutants of E. coli. The results of restriction endonuclease digestion and Southern hybridization analysis indicate that the relevent leu and pro biosynthetic genes of A. magnetotacticum are not closely linked on the chromosome.
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References
Bachmann BJ (1983) Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev 47:180–230
Bates PF, Swift RA (1983) Double cos site vectors: simplified cosmid cloning. Gene 26:137–146
Blakemore RP (1975) Magnetotactic bacteria. Science 190:377–379
Blakemore RP, Maratea D, Wolfe RS (1979) Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium. J Bacteriol 140:720–729
Blakemore RP, Frankel RB, Kalmijn AdJ (1980) South-seeking magnetotactic bacteria in the Southern Hemisphere. Nature 286:384–385
Bolivar F, Rodriguez RL, Grenne PJ, Betlach MC, Heynecker HL, Boyer W, Crosa JH, Falkow S (1977) Construction and chartacterization of new cloning vehicles. Gene 2:95–113
Csonka LN, Baich A (1983) Proline biosynthesis. In: Herrnann K, Somerville RL (eds) Amino acid biosynthesis and genetic regulation. Addison-Wesley, Reading, Mass, pp 35–51
Datta AR, Ostroff R, MacQuillan AM (1987) Genetic and physical characterization of proBAgenes of the marine bacterium Vibrio parahaemolyticus. Appl Environ Microbiol 53:2733–2738
Fons M, Cami B, Patte JC, Chippaux M (1987) Cloning in Escherichia coli of genes involved in the synthesis of proline and leucine in Desulfovibrio desulfuricans Norway. Mol Gen Genet 206:141–143
Frankel RB, Blakemore RP, Wolfe RS (1979) Magnetite in freshwater magnetotactic bacteria. Science 203:1355–1356
Frankel RB, Blakemore RP, Torres de Araujo FF Esquival DMS, Danon J (1981) Magnetotactic bacteria at the geomagnetic equator. Science 212:1269–1270
Hayzer DJ, Lensinger T (1980) The gene-enzyme relationship of proline biosynthesis in Escherichia coli. J Gen Microbiol 118:287–293
Maratea D, Blakemore RP (1980) Aquaspirillum magnetotacticum sp. nov., a magnetic spirillum. Int J Syst Bacteriol 31:452–455
Moench TT, Konetzka WA (1978) A novel method for the isolation and study of magnetotactic bacteria. Arch Microbiol 119:203–212
Rodriguez RL, Tait RC (1983) Recombinant DNA techniques: An introduction. Addison-Wesley, Reading, Mass
Staskwicz B, Dahlbeck D, Keen N, Naploi C (1987) Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. J Bacteriol 169:5789–5794
Taylor AL, Thoman MS (1964) The genetic map of Escherichia coli K-12. Genetics 50:659–677
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Communicated by H. Böhme
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Waleh, N.S. Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12. Mol Gen Genet 214, 592–594 (1988). https://doi.org/10.1007/BF00330500
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DOI: https://doi.org/10.1007/BF00330500