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  • Chemistry  (3,458)
  • Polymer and Materials Science  (1,616)
  • Animals  (615)
  • 1980-1984  (3,349)
  • 1960-1964  (942)
  • 1930-1934  (118)
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Year
  • 1
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    Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine (1981)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1981-12-04
    Description: A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When Escherichia coli transformed with this plasmid was grown in tryptophan-depleted media, approximately 17 percent of the total cellular protein was found to be an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to foot-and-mouth disease virus. When inoculated into six cattle and two swine, this protein elicited high levels of neutralizing antibody and protection against challenge with foot-and-mouth disease virus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kleid, D G -- Yansura, D -- Small, B -- Dowbenko, D -- Moore, D M -- Grubman, M J -- McKercher, P D -- Morgan, D O -- Robertson, B H -- Bachrach, H L -- New York, N.Y. -- Science. 1981 Dec 4;214(4525):1125-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6272395" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Antibody Formation ; Base Sequence ; Cattle ; Cattle Diseases/*prevention & control ; *Cloning, Molecular ; DNA Restriction Enzymes ; DNA, Recombinant/metabolism ; Foot-and-Mouth Disease/*prevention & control ; Immunity, Cellular ; Protein Biosynthesis ; Swine ; Swine Diseases/*prevention & control ; Transcription, Genetic ; *Vaccines ; Viral Proteins/genetics/*therapeutic use
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Gas chromatography mass spectrometry computer analysis of volatile halogenated hydrocarbons in man and his environment - a multimedia environmental study (1980)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 7 (1980), S. 139-147 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: As part of a study to make a comparative analysis of selected halogenated compounds in man and the environmental media, a quantitative gas chromatographic mass spectrometric analysis of the levels of the halogenated compounds found in the breath, blood and urine of an exposed population (Old Love Canal area, Niagara, New York) and their immediate environment (air and water) was undertaken. In addition, levels of halogenated hydrocarbons in air samples taken in the general Buffalo, Niagara Falls area were determined.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200070402
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  • 3
    Electronic Resource
    Electronic Resource
    Cultivation of a filamentous mold in a glass pilot-scale airlift fermentor (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 863-877 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Results of pilot plant studies using a glass airlift fermentation device (55 liter fermentation volume) have proven the relative merits of such a system in the fermentation of a filamentous mold, Monascus purpureus, on 4% (w/w) starch media. The resultant overall yield of cell mass (Yx/s) of 0.38 was an appreciable increase over the 0.32 obtained with a pilot scale stirred tank fermentor previously studied. Power requirements of the airlift fermentor were approximately 50% of those for the mechanically agitated system. The lack of mechanical shear in the airlift system provides a more gentle environment or the cultivation of organisms than does the high degree of shear prevalent in the mechanically agitated vessels. Mass transfer of oxygen to the aqueous phase of the fermentation volume is improved significantly through use of the airlift device. Mass transfer coefficients in the range of 200 reciprocal hr were obtained to approximately 80 reciprocal hr in the stirred tank fermentor.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260230416
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of solute purity, temperature, and surfactants on solid-liquid mass transfer (1963)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 9 (1963), S. 474-479 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690090412
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  • 5
    Electronic Resource
    Electronic Resource
    Deterioration of plastic films under soil burial conditionsIssued as N.R.C.C. # 18807. (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 26 (1981), S. 509-519 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The deterioration of polyolefin, poly(ethylene terephthalate), and polyamide films under soil burial conditions extending up to 32 months has been investigated. Based on changes in their elongation at break, the films can be ranked in order of increasing sensitivity to degradation: Polyester ≃ polypropylene 〈 low-density polyethylene ≃ high-density polyethylene 〈 nylon 6.6. The degraded nylon 6.6 and polyethylene films were characterized by infrared and luminescence spectroscopy and scanning electron microscopy, as well as by wet analysis for hydroperoxides. From a comparison with the well-known oxidative sensitivity of nylon 6.6 in oxygenated water at slightly elevated temperatures, the rapid deterioration of nylon 6.6 film during soil burial was also concluded to be an oxidative process. The somewhat smaller, but significant, embrittlement of the polyethylenes studied could not be simply explained by thermal oxidation (with only trace oxidation products detectable) or microbiological attack (deterioration being unaffected by surface activation to enhance wettability).
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1981.070260211
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  • 6
    Electronic Resource
    Electronic Resource
    Blood-materials interactions: The minimum interfacial free energy and the optimum polar/apolar ratio hypotheses (1982)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 16 (1982), S. 381-398 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Numerous hypotheses exist to explain observed blood-materials interactions. It is the purpose of this article to test two popular hypotheses, namely, the minimum interfacial free energy hypothesis and the optimum polar/apolar ratio hypothesis. Methacrylate polymers and copolymers were characterized using the captive bubble underwater contact angle method; bulk water content was determined by gravimetric methods; streaming potential measurements were made; and surface roughness and possible particulate contamination were evaluated by reflected light microscopy. In vitro blood tests include whole blood clotting time measurements on polymer-coated tubes; centrifugal force platelet adhesion on polymer-coated coverslips; and a measure of the partial thromboplastin time, Russell's viper venom time (Stypven time), and the prothrombin time of native whole blood exposed to polymer-coated microscope slides. Results suggest that platelet adhesion correlates in the opposite direction of whole blood clotting time and partial thromboplastin time, emphasizing the need for a multiparameter approach to blood-materials testing. Based on these tests the minimum interfacial free energy hypothesis is not supported. In fact, the data suggest the opposite to be true. It is apparent that platelet adhesion can be a misleading indicator of blood compatibility. Neither hypotheses can explain the apparent conflict between the platelet adhesion data and the coagulation time data.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820160407
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  • 7
    Electronic Resource
    Electronic Resource
    Fracture of porous polyethylene-bone composite (1983)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 17 (1983), S. 59-70 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Porous high-density polyethylene specimens were implanted in the femurs of mongrel canines. At the end of the residency period (3 or 6 months), the animals were sacrificed and the implants were retrieved. The work-of-fracture of the implant specimens was then determined using the technique of Tattersall and Tappin. The work required to fracture a specimen in three-point bending by controlled crack propagation through a triangular cross section was obtained directly from the load-deflection curve. The area of the resulting fracture surface was measured by macro-photographic techniques, and the work-of-fracture was calculated as work per unit area. The implants were subsequently sectioned and examined microradiographically to determine the extent of bone ingrowth. Bone specimens adjacent to the implants and porous high-density polyethylene controls (no ingrowth) were also tested to determine their work-of-fracture. The results showed that bone adjacent to the implant specimens had a higher work-of-fracture than normal medial, canine femoral bone and was not appreciably different from the composite. The work-of-fracture of porous high-density polyethylene was not significantly increased by an increase in bone infiltration, and this anomalous behavior was attributed to a degradation of the polyethylene during implant residence. Control studies supported this hypothesis.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820170106
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  • 8
    Electronic Resource
    Electronic Resource
    High performance liquid chromatography (HPLC) of natural products part VI.For part V, see [1] Sulfoxides of penicillin N and cephalosporin c and their role in biosynthesis of β-lactam antibiotics (1984)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 67 (1984), S. 876-884 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Pure pencillin N α-sulfoxide (1) and penicillin N β-sulfoxide (2) were obtained by HPLC and tested as substrates for deacetoxycephalosporin C synthetase (DXCS). Neither one of the sulfoxides was utilized under conditions of conversion of penicillin N (8) to deacetoxycephalosporin C (9). The cephalosporin C α and β-sulfoxides (3 and 4, resp.) were also prepared. Relative stabilities of the sulfoxides 3 and 4 are discussed by interpretation of the 13C-NMR spectra.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19840670329
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  • 9
    Electronic Resource
    Electronic Resource
    Spectroscopic studies of solutions, suspensions, and precipitates of poly(1,6-di-p-toluene sulfonyloxy-2,4-hexadiyne) (1981)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Physics Edition 19 (1981), S. 321-334 
    Details
    ISSN: 0098-1273
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Optical-absorption, fluorescence, and Raman spectra for solutions, suspensions, and precipitates of poly(1,6-di-p-toluene sulfonoxy-2,4-hexadiyne) in and from nitrobenzene, acetone, and chloroform are presented. These are interpreted in terms of the occurrence of two forms of the polymer chain; a quasicrystalline form with properties close to those of single crystal polymer and a chain-extended form occurring in solution and colloidal particles, with an absorption energy of about 2.5 eV (20,000 cm-1). No evidence is found for the presence of very short polymer chains in partially polymerized monomer at low conversion. The relationship of these results to those for deformed single crystals is briefly discussed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1981.180190212
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  • 10
    Electronic Resource
    Electronic Resource
    Mean free path for inelastic scattering of 1.2 kev electrons in thin poly(methylmethacrylate) films (1980)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 2 (1980), S. 5-10 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Mean free paths for inelastic scattering (λ) of low energy electrons in poly(methylmethacrylate) (PMMA) have been determined by measuring Al Kα excited C 1s and Si 2s photoelectron signal intensities as a function of ellipsometrically determined thicknesses of PMMA overlayers on silicon substrates. The λ values obtained are 29 ± 4 Å for 1196 eV electrons and 33 ± 5 Å for 1328 eV electrons. These data are necessary for the quantitative analysis of the surface region of PMMA and similar polymers by X-ray photoelectron spectroscopy (XPS). The magnitude of the λ values determined indicates that XPS measurements can provide chemical information about the surface region of polymers, such as PMMA, in the depth range of ∼6 to 100 Å. The results of this study are compared and discussed with respect to λ values determined for other organic compounds.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740020103
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