ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The mathematical model for the reaction sequence catalyzed by immobilized invertase and glucose oxidase discussed in the preceding article has been used successfully to duplicate experimental findings. In addition, it has been used as a tool for the simulation and prediction of effects derived from alterations to system-related and gel-related parameters. The effects of gel diffusivity on the overall conversion of sucrose substrate to reaction products was investigated through use of this model. Changes in the enzyme loading within a gel and the results of varying the ration of invertase activity to glucose activity were also evaluated. Through use of concentrations of the molecular species determined at the collocation points within a gel particle and in the bulk liquid phase, an estimate of the thickness of the diffusion boundary layer around the gel particle was determined which was in close agreement with values obtained from classical mass transfer relationships. For most of this study, the enzymes were coimmobilized within the same polymeric matrix. However, a number of tests were run with the enzymes immobilized individually and placed in separate reactors in a sequential reactor system. The experimental results from these tests were duplicated successfully by means of the model with little modification to the basic computer program. Such an example illustrates the potential flexibility of the model and its overall versatility.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260231107
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