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  • 1
    Electronic Resource
    Electronic Resource
    A behavioural and electrophysiological study of the role of tarsal chemoreceptors in feeding by adults of Spodoptera, Heliothis virescens and Helicoverpa armigera
    Amsterdam : Elsevier
    Journal of Insect Physiology 36 (1990), S. 743-745+747-756 
    Details
    ISSN: 0022-1910
    Keywords: Helicoverpa armigera ; Heliothis virescens ; Spodoptera frugiperda ; Spodoptera littoralis ; adult feeding ; amino acids ; azadirachtin ; behaviour ; electrophysiology ; neural interactions ; plant extracts ; sugars ; tarsal chemoreceptors
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0022-1910(90)90048-K
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  • 2
    Electronic Resource
    Electronic Resource
    Induction of embryogenesis with colchicine instead of heat in microspores ofBrassica napus L. cv. Topas (1996)
    Springer
    Planta 198 (1996), S. 433-439 
    Details
    ISSN: 1432-2048
    Keywords: Brassica napus ; Colchicine ; Heat shock ; Microspore embryogenesis ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis fromBrassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction inB. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00620060
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  • 3
    Electronic Resource
    Electronic Resource
    High frequency production of doubled haploid plants of Brassica napus cv. Topas derived from colchicine-induced microspore embryogenesis without heat shock (1996)
    Springer
    Plant cell reports 15 (1996), S. 668-671 
    Details
    ISSN: 1432-203X
    Keywords: Brassica napus ; Chromosome doubling ; Colchicine ; Doubled haploids ; Microspore embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This report describes a very high genome doubling efficiency of Brassica napus cv. Topas plants, derived from microspores induced to undergo embryogenesis with a colchicine treatment, without the use of a heat treatment. The plants showed normal growth and development, and 90% were fertile. In contrast, only 6% of the plants derived from heat-induced embryos were fertile diploids. All cytological analysis of the progeny of fertile plants showed 2n=38 chromosomes. These results show that colchicine can simultaneously induce microspore embryogenesis and double the ploidy level to produce doubled haploid plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231921
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of culture density, conditioned medium and feeder cultures on microspore embryogenesis in Brassica napus L. cv. Topas (1990)
    Springer
    Plant cell reports 8 (1990), S. 594-597 
    Details
    ISSN: 1432-203X
    Keywords: Brassica napus ; microspore ; density ; conditioned medium ; feeder ; embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In microspore cultures of Brassica napus L. cv. Topas, embryo yield increases with culture density up to about 40,000 microspores per ml. A much higher density (100,000 per ml) appears inhibitory to embryogenesis. A relatively high culture density (30,000 or 40,000 per ml) for the first 2–4 days of culture is crucial for embryogenesis, after which cultures may be diluted to allow better embryo growth. Medium conditioned by culturing microspores at 30,000 or 40,000 per ml for 1 day improved microspore-embryo yield in low density cultures (3,000 or 4,000 per ml) more than 3-fold. In contrast, media conditioned with microspores from 1–4 days or 0–4 days of culture were inhibitory. Use of feeder cultures resulted in up to 10-fold increase of embryo yield in low density microspore cultures, depending on the method used. Filter papers and other membranes placed on top of feeders greatly inhibited embryogenesis in the feeder layer as well as microspores cultured on the feeder, possibly due to poorer gaseous exchange.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270061
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  • 5
    Electronic Resource
    Electronic Resource
    Azadirachtin: structural requirements for reducing growth and increasing mortality in lepidopterous larvae (1990)
    Springer
    Entomologia experimentalis et applicata 55 (1990), S. 169-181 
    Details
    ISSN: 1570-7458
    Keywords: Azadirachtin ; Spodoptera littoralis ; Spodoptera frugiperda ; Heliothis virescens ; growth disruption ; oral cannulation ; abdominal injection ; topical application
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Spodoptera littoralis, S. frugiperda et Heliothis virescens ont été exposés à l'azadirachtine et 23 autres substances voisines dans 3 expériences: canulation orale, injection d'haemolymphe et application topique. S. littoralis est sensible à un plus grand nombre de substances que les 2 autres espèces. De toutes façons, les substances les plus actives ont été l'azadirachtine, la 22,23-dihydroazadirachtine et la 1-détigloyl-22,23-dihydroazadirachtine. La nature des substitutions en C-1 et C-3 de l'anneau décaline modifie la puissance des substances, tout comme les substitutions en C-22,23. D'une façon générale, les chenilles ont été moins sensibles aux applications topiques qu'aux canulations dans le tube digestif ou aux injections dans l'haemolymphe.
    Notes: Abstract Spodoptera littoralis, Spodoptera frugiperda and Heliothis virescens were exposed to azadirachtin and 23 other related compounds in three bioassays: oral cannulation, haemolymph injection and topical application. Spodoptera littoralis was susceptible to a larger proportion of the compounds than either of the other species. Overall, azadirachtin, 22,23-dihydroazadirachtin and 1-detigloyl-22,23-dihydroazadirachtin were the most active compounds. The nature of the substitutes at C-1 and C-3 of the decalin ring affects the potency of the compounds, as does the addition of substitutes to C-22,23. Generally, larvae were less sensitive to compounds when they were topically applied than when they were cannulated into the gut or injected into the haemolymph.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352578
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  • 6
    Electronic Resource
    Electronic Resource
    Antifeedant effects of azadirachtin and structurally related compounds on lepidopterous larvae (1990)
    Springer
    Entomologia experimentalis et applicata 55 (1990), S. 149-160 
    Details
    ISSN: 1570-7458
    Keywords: Spodoptera littoralis ; Spodoptera frugiperda ; Heliothis virescens ; Heliothis armigera ; azadirachtin ; salannin ; limonoids ; antifeedants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'activité phagodissuadante de l'azadirachtine, de ses dérivés et des limonoïdes voisins sur 4 espèces de lépidoptères: Spodoptera littoralis, S. frugiperda, Heliothis virescens et H. armigera a été évaluée par des expériences avec et sans choix. Les expériences de choix ont montré que le comportement alimentaire de S. littoralis était modifié par plus de substances que celui de S. frugiperda ou H. virescens. Celui de H. armigera était le moins modifié. Les 2 substances les plus puissantes parmi les 40 examinées, ont été l'azadirachtine et le dihydroazadirachtine. Ces résultats montrent que l'hydrogénation de la double liaison C-22,23 ne réduit pas l'activité phagodissuadante et que la nature des substitutions en C-1, C-3 et C-11 sont importantes. Les molécules avec des substitutions volumineuses en C-22 ou C-23 sont généralement des phagodissuadants aussi inefficaces que ceux ayant perdu un époxide. Les substances notées comme phagodissuadants actifs dans les expériences de choix ne sont pas toujours aussi actives en absence de choix. La valeur des tests dans l'évaluation du mode d'action des substances est discuté.
    Notes: Abstract The antifeedant activity of azadirachtin, azadirachtin-derivatives and related limonoids was assessed in choice and no-choice bioassays against four species of Lepidoptera: Spodoptera littoralis, Spodoptera frugiperda, Heliothis virescens and Heliothis armigera. The choice bioassay showed that the feeding behaviour of S. littoralis was affected by more of the compounds than that of either S. frugiperda or H. virescens. H. armigera was the least affected. Azadirachtin and dihydroazadirachtin were the most potent of the 40 compounds tested. The results showed that hydrogenation of the C-22,23 double bond did not decrease antifeedant activity and the nature of the substitutes at C-1, C-3 and C-11 were important. Molecules with bulky substitutes at either C-22 or C-23 were usually ineffective antifeedants as were compounds lacking an epoxide. Compounds recorded as active antifeedants in the choice bioassay were not always as active in the no-choice test. The value of the bioassays in assessing the mode of action of the compounds is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352576
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  • 7
    Electronic Resource
    Electronic Resource
    The role of the secondary plant compound 2,5-dihydroxymethyl 3,4-dihydroxypyrrolidine as a feeding inhibitor for insects (1984)
    Springer
    Entomologia experimentalis et applicata 36 (1984), S. 209-216 
    Details
    ISSN: 1570-7458
    Keywords: Secondary plant compounds ; 2,5-dihydroxymethyl ; 3,4-dihydroxypyrrolidine ; Spodoptera littoralis ; S. exempta ; Heliothis virescens ; Schistocerca gregaria ; Locusta migratoria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Récemment a été mis en évidence le puissant pouvoir inhibiteur de différents glucosidases. Présenté par l'amino-alditol cyclique, 2,5 dihydroxymethyl 3,4 dihydroxypyrrolidine (DMDP), isolé de graines de la légumineuse Lonchocarpus sericeus. Nous avons examiné le rôle de ce composé secondaire dans la stratégie défensive de la plante contre une gamme d'insectes phytophages, à travers son action sur le développement, le comportement alimentaire et le fonctionnement des récepteurs gustatifs. Nous avons montré que DMDP peut inhiber, suivant la dose, la prise de nourriture et, qu'apr⪻s ingestion (par Spodoptera spp), il peut agir comme une toxine. Par suite de sa structure moléculaire, DMDP peut être considéré, non seulement comme l'analogue d'un sucre, mais aussi comme un alcaloïde. Le bilan des résultats de nos expériences suggère que l'efficacité de DMDP comme phagodissuadant, si elle ne provient pas de sa toxicité, découle principalement de ses propriétés d'alcaloïde.
    Notes: Abstract The cyclic amino alditol 2,5-dihydroxymethyl 3,4-dihydroxypyrrolidine (DMDP) has recently been shown to be an inhibitor of various glucosidases. We have investigated its role as a plant protective chemical against a range of phytophagous insects by examining its effects on development, feeding behaviour and functioning of taste receptors. We have shown that it can inhibit feeding and can be toxic. The mechanisms by which these effects are achieved are discussed in the context of the molecular structure of the compound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00185649
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  • 8
    Electronic Resource
    Electronic Resource
    Food selection in adults and larvae of three species of Lepidoptera: a behavioural and electrophysiological study (1988)
    Springer
    Entomologia experimentalis et applicata 49 (1988), S. 111-121 
    Details
    ISSN: 1570-7458
    Keywords: Spodoptera littoralis ; Heliothis virescens ; H. armigera ; electrophysiology ; feeding behaviour ; adults ; larvae ; proboscis sensilla ; antifeedants ; phagostimulants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adults and larvae of Spodoptera littoralis, Heliothis virescens and Heliothis armigera were tested with a range of sugars, amino acids, sugar alcohols and allelochemics. Feeding behaviour was correlated with the electrophysiological responses of maxillary styloconic sensilla in the larvae and proboscis styloconic sensilla in the adult. The neural response was more vigorous in larvae than in adults but otherwise the spectra of responses were similar in the two life stages. Phagostimulants and antifeedants stimulated maximally different sensilla in larvae but not in adults. The responses of adult sensilla to sugars and amino acids were significantly correlated to behaviour in all three species, but only in H. armigera was there a similar correlation with the sugar alcohols and allelochemics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00188244
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  • 9
    Electronic Resource
    Electronic Resource
    Behavioral and electrophysiological study of antifeedant mechanisms associated with polyhydroxy alkaloids (1990)
    Springer
    Journal of chemical ecology 16 (1990), S. 3167-3196 
    Details
    ISSN: 1573-1561
    Keywords: Spodoptera littoralis ; Spodoptera exempta ; Heliothis virescens ; Helicoverpa armigera ; Lepidoptera ; Noctuidae ; polyhydroxy alkaloids ; piperidine ; pyrrolidine ; castanospermine ; swainsonine ; alexine ; fagomine ; behavior ; electrophysiology ; receptor mechanisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Eleven polyhydroxy alkaloids of plant origin were tested for anti-feedant effects against larvae of the lepidopteransSpodoptera littoralis, Spodoptera frugiperda, Heliothis virescens, andHelicoverpa armigera. Data from behavioral and electrophysiological investigations were correlated to reveal information on the mode of action of the antifeedants. The pyrrolidine DMDP was an effective antifeedant for all four species, whereas the piperidines fagomine and XZ-1 and the pyrrolizidine alexine were all ineffective as antifeedants. The activity of the pyrrolidines CYB-3 and DAB-1, the piperidines DNJ, DMJ, and BR1, and the bicyclic octahydroindolizine castanospermine varied among species. The investigation focuses on the structural similarities between some of the alkaloids and some common phagostimulatory sugars and illustrates a neural interaction involving the neurons that are differentially responsive to alkaloids and sugars. InS. littoralis, the neurons responding specifically to the alkaloids DMDP, DAB-1, and castanospermine and to the sugars fructose, sucrose, and glucose are more active when the compounds are applied singly than when an alkaloid and a sugar are applied together. The implications for the occurrence and functioning of different sugar receptor sites are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00979618
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  • 10
    Electronic Resource
    Electronic Resource
    A procedure for the microinjection of plant cells and protoplasts (1989)
    Springer
    Methods in cell science 12 (1989), S. 139-144 
    Details
    ISSN: 1573-0603
    Keywords: microinjection ; genetic transformation ; protoplasts ; microspores ; Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This paper describes a general method suitable for the microinjection ofBrassica napus protoplasts, unicellular microspores, and multicellular microspores. By incorporating components taken from other methods, manual operations frequently involved in the microinjection of plant cells have been simplified and microinjection rates increased. The embedding of cells in agarose provides a simple alternative to the variety of sophisticated immobilization strategies devised for different plant cell types thereby reducing the manipulations often involved in the culture of microinjected cells. Use of an automatic microinjector eliminated the operation of fine control systems on manual injectors; however, precision in sample delivery was reduced. Analyses indicate that transformed tissues can be recovered from microinjected protoplasts and microspores at high frequencies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404440
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