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  • 1
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    Sperm fertilization capacity of Caspian salmon, Salmo trutta caspius under ultraviolet irradiation (2021)
    In:  http://aquaticcommons.org/id/eprint/21728 | 18721 | 2017-11-28 08:45:40 | 21728 | University of Guilan, Faculty of Natural Resources, Iran
    Details
    Publication Date: 2021-06-29
    Description: The optimum dose of ultraviolet (UV) irradiation to produce gynogenetic Caspian salmon, Salmo trutta caspius, with emphasis on the "Hertwig effect" and photoreactivation (PR) were investigated. The sperm of Caspian salmon was irradiated with UV at 2010 ± 200 µw.cm-2 in different times including 0, 1, 3, 5, 8, 10, 15, 20, 25, 35 and 45 min and was allowed to fertilize normal ova; the fertilization, eyed and hatching rates were calculated to assess the performance. Using the irradiated sperm decreased the fertilization, eyed as well as hatching rates and the so-called "Hertwig effect" was observed, with the time-dependent decrease in the hatching rates at 0+ to 3 min irradiation, but better hatching rates were observed at more prolonged irradiation times. The best hatching rate was achieved at 25 min of UV irradiation; after that the survival rates rapidly declined to near zero. For PR studies, the semen was irradiated with UV (5, 30 and 120s) and untreated semen (0s) was used as control. Irradiated semen and/or fertilized eggs by treated semen were exposed to visible light (60 W) at a distance of 30 cm for 10 min; the eyed and hatching rates were measured. UV irradiation as low dose as 5s, significantly decreased the hatching rate (P 〈0.05). Semen and/or eggs illumination with visible light could not improve the survival rates (P 〉0.05). So, based on the results of this study, it was impossible to detect any PR mechanism in Caspian salmon.
    Keywords: Aquaculture ; Biology ; Salmo trutta caspius ; sperm ; fertilization ; capacity ; Caspian salmon ; ultraviolet ; irradiation ; Caspian sea ; iran ; photoreactivation ; gynogenetic
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
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    Format: 1-13
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  • 2
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    Genetic variability and differentiation of common Kilka fish (Clupeonella cultriventris Nordmann, 1840) in the southern coasts of Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/21944 | 18721 | 2018-01-18 08:55:31 | 21944 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: A total of 120 samples of adult common Kilka fish (Clupeonella cultriventris) were collected during spring and summer from the southern coasts of Caspian Sea (Bandar Anzali and Babolsar). Fifteen sets of microsatellite primers were developed from Clupeidae being tested on genomic DNA of common Kilka. Allele frequency, observed and expected heterozygosity, FST, RST, FIS index were determined. Five primer sets as polymorphic loci were used to analyze the genetic variation in adults of the common Kilka population. Results revealed that average alleles per locus was 13.1 (range 5 to 22 alleles per locus in regions, Ne=9.5). All sampled regions contained private alleles. Average observed and expected heterozygosity was 0.348 and 0.877, respectively. Deviations from Hardy Weinberg equilibrium were observed in most cases. FST, RST and gene flow estimates in AMOVA and the genetic distance between populations indicated that the genetic difference among the studied populations was pronounced. The data generated in this study provide primary information on the genetic variation and differentiation in populations of Caspian common Kilka.
    Keywords: Biology ; Fisheries ; Population ; genetic ; Microsatellite ; Clupeidae ; Common kilka ; Clupeonella cultriventris ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
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    Format: 139-148
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  • 3
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    Genetic relationships of Iranian coastline ship sturgeon (Acipenser nudiventris) samples and Ural population based on microsatellite DNA (2021)
    In:  http://aquaticcommons.org/id/eprint/22458 | 18721 | 2018-04-09 17:47:06 | 22458 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: Genetic relationships among 96 specimens of ship sturgeon, Acipenser nudiventris, collected from six locations along the Iranian coastline as well as 8 specimens from northern population (Ural River) were analysed by the restricted maximum likelihood method, as well as two distance analysis method (Nei's and Cavalli-Sforza's distance) of gene frequencies. We have used four SSR markers that produced three polymorphic and two monomorphic loci. Overall results showed that Iranian coastline samples form a monophyletic group (clade) which was different from the northern (Ural) samples. Iranian coastline samples are divided into two groups (clades): Anzali-Kiashahr clade and Sefidrud-Babolsar-Noushahr-Gorgan clade. Bootstrapping test showed monophyleticity of Sefidrud, Babolsar, Noushahr, Gorgan with high (Bootstrap support=93) and Anzali-Kiashahr with middle (Bootstrap support=65) confirmation. Topology of reconstructed trees was in correspondence with geographical distributions of samples.
    Keywords: Biology ; Fisheries ; Ship sturgeon ; Acipenser nudiventris ; Genetic relationships ; Microsatellite DNA ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
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    Format: 229-240
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  • 4
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    Application of microsatellite markers to determine populations of the Persian sturgeon (Acipenser persicus) in the south of Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/22465 | 18721 | 2018-04-10 21:21:52 | 22465 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-06
    Description: The objective of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) in Sefidrud and Gorganrud rivers watershed based on the characterization of microsatellite markers during 2006 - 2008. 100 samples of Persian sturgeon were collected from two regions. Four microsatellite loci (Ls68, Spl168, Spl173 and Afu68) were analyzed for the molecular characterization of this species which resulted in polymorphic patterns. DNA bands were analysed using Biocapt and GenAlex software package. A total of 109 alleles were observed of which the maximum number of alleles (17) were found in Spl168 locus which belonged to sturgeons from Sefidrud river's watershed and the minimum number of alleles (10) in Ls68 locus belonging to the sturgeons from Gorganrud river's watershed. Results of microsatellite analysis revealed that the differences between samples of two regions were not statistically significant (p〉0.05), neither for the average number of alleles per locus nor for observed heterozygosities. The calculated Fst and Rst between two regions was 0.07 and 0.17 showing that the genetic difference was significant (p〈 0.01). Samples from Sefidrud river's watershed in Spl173, Afu68 and Spl168 loci and samples of other regions in Afu68 and Spl168 loci were at Hardy-Weinberg equation. The genetic distance was calculated as 0.4 which represents a significant genetic difference between samples of two studied areas. In conclusion, this study suggests that the Persian sturgeons in two regions of the southern part of the Caspian Sea are genetically differentiated, therefore fisheries management of these unique stocks for restocking and conservation of gene pools is highly recommended.
    Description: Article includes abstract in Farsi on last page.
    Keywords: Biology ; Fisheries ; Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Genetic structure ; Population genetic ; Iran
    Repository Name: AquaDocs
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    Format: application/pdf
    Format: application/pdf
    Format: 596-606
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  • 5
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    Karyotype analysis in white bream (Blicca bjoerkna transcaucasica) from north coast of Iran (2021)
    In:  http://aquaticcommons.org/id/eprint/22390 | 18721 | 2018-04-03 13:13:58 | 22390 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-04
    Description: Preparation of chromosome spreads and karyotype analysis in Blicca bjoerkna transcaucasica were carried out using 0.01% solution of colchicines and Phytohaemagglutinin (PHA) (20 µg g-1 body weight). The gill and kidney tissues were collected and let to stand in a hypotonic solution of 0.075 M KCl and then treated with a fixative (Carnoy's solution) in three steps. The chromosomes spreads were then stained with 5% Giemsa solution for 20 min and examined under a light microscope. Appropriate metaphase plates were photographed in order to prepare karyotype. The size of the chromosomes (short and long arms), relative length of chromosomes and centromere index were calculated. Chromosome spreads from gill tissue cultures which were colchicine treated with PHA, had a well defined size, shape and number of chromosomes for karyotype analysis. Based on the 76 metaphase plates studied, chromosome count in 59 metaphase plates was 2n=49.74±0.25. By arranging homologous chromosomes beside each other the chromosome formula was calculated as 6 pairs of Metacentric, 10 pairs of Sub-Metacentric and 9 pairs of Sub-Telocentric (2n=6M+10+Sm+9St) and the chromosome arm number (NF) was 100. The largest chromosome in this species was a pair of metacentric chromosomes. On the basis of the number and type of chromosomes, the karyotype obtained for this species conformed to the findings of other researchers, but the chromosome formula was different, which could be attributed to the existence of different populations for this species.
    Keywords: Biology ; Fisheries ; Blicca bjoerkna transcaucasica ; Phytohaemagglutinin ; Colchicines ; Karyotype ; Iran
    Repository Name: AquaDocs
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    Format: application/pdf
    Format: application/pdf
    Format: 454-463
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  • 6
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    Persian sturgeon growth hormone elaboration and purification (2021)
    In:  http://aquaticcommons.org/id/eprint/22837 | 18721 | 2018-05-25 22:34:14 | 22837 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-10
    Description: In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 µL isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours and after bacterial cells lysis crude extracts with recombinant proteins inclusion bodies (IB) were loaded on 15% SDS-PAGE gel. Thenafter staining, comparative concentrations of rpsGH were measured by densitometric scanning of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel it was more than 90 %. The maximum yield of GH was observed after 4 hours of growth. To recover active psGH from inclusion bodies we used imidozole to obtain most of the total recombinant protein in the soluble fraction. Purification of 6xhisN tag recombinant psGH has been performed using affinity chromatography where nickel was bound to an agarose bead by chelation using NTA (nitrilotriacetic acid) beads. The overall yield of the purified monomeric psGH was approximately 50% of the initial IB proteins. The purification manipulations including IB isolation and solubilisation, protein refolding by dialyze and affinity chromatography ensure yields of biologically active psGH up to 30%. This study shows that, the affinity chromatography is a powerful and very specific method for recombinant proteins purification of psGH.
    Keywords: Biology ; Chemistry ; Fisheries ; Persian sturgeon ; Growth hormone ; Purification ; Affinity chromatography ; Biotechnology ; Iran
    Repository Name: AquaDocs
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    Format: 1053-1060
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  • 7
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    Karyology of pikeperch (Sander lucioperca)in the south Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/23652 | 18721 | 2018-07-15 00:13:02 | 23652 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-14
    Description: Pikeperch (Sander lucioperca) is one of the economic fish species in the Caspian Sea, with no official report on its chromosome preparation and karyotype formula. For the metaphase preparation, 29 fry specimens of the fish weighting 10-25g each were used. Colchicine 0.01% at 0.7ml/100g of fish weight was injected in the muscular tissues. After 180 minutes, kidney and gill tissues were removed and hypotonized in KCL 0.075 M for 40 minutes, fixed with Carnoy mixture for 18-20 hours, homogenized and dropped on the slides, and stained with Giemsa 20% for 30 minutes. The prepared slides were swept by objective X100 light photomicroscope. The results showed that the chromosome number of this species is 2n = 48 and the chromosome arms is NF = 76. The karyotype formula of the species was found to be 2n = 1m + 13sm + 4st + 6a. Comparing the results of the study with those of other researchers showed that the pikeperch chromosome number in the South Caspian Sea is similar to the fish in other parts of the world but the type of chromosomes and karyotype formula is different.
    Keywords: Biology ; Chromosomes ; Economics ; Kidney ; Gills ; Colchicine ; Light microscopy ; Commercial species ; Karyotypes ; Sander lucioperca ; Metaphase ; potassium chloride ; Chromosome number ; Brackish
    Repository Name: AquaDocs
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    Format: 137-144
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  • 8
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    Introducing molecular markers for Lernaea cyprinacea and Lernaea ctenopharyngodoni using RAPD technique (2021)
    In:  http://aquaticcommons.org/id/eprint/23658 | 18721 | 2018-07-15 07:37:24 | 23658 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-14
    Description: Molecular comparison of two parasites Lernaea cyprinacea and Lernaea ctenopharyngodoni was carried out using RAPD (Random Amplified Polymorphic DNA) technique. A total of 43 Lernaea specimens belonging to the two species were collected from the Guilan and Khouzestan Provinces. DNA was extracted using the Phenol-chloroform method. The quality and quantity of DNA was assessed using 1% Agarose gel electrophoresis and spectrophotometer. Polymerase Chain Reaction (PCR) was conducted on the target DNA under specific conditions and PCR products were subjected to electrophoresis on polyacrylamide gels (6%). Polyacrylamide gels were stained using silver nitrate and DNA bands were analyzed with BioCapt software. The genetic analysis was conducted using POP GEN 32 software. Forty two primers, 10 nucleotides each were used for PCR reaction. Totally, 397 RAPD loci were counted on polyacrylamide gel where 349 identical loci were polymorphic of which some bands may be used as genetic markers for the identification of both Lernaea species. Data analysis on PCR products showed higher genetic variation (1.15%) of L. ctenopharyngodon in the Guilan Province as compared to that of the Khouzestan (0.0%). However, genetic variation (27.46%) of L. cyprinacea in the Khouzestan Province was 7.26 times higher than that of the Guilan province (3.78%). The two species showed a genetic differentiation of approximately 88%. Based on the observed molecular differences, we state that L. ctenopharyngodoni is a genetically independent species from L. cyprinacea.
    Keywords: Biology ; Molecular structure ; Genetics ; DNA ; Freshwater ; Parasites ; Polymerase chain reaction ; Primers ; Nucleotides ; Lernaea ctenopharyngodoni ; Lernaea cyprinacea ; Gilan Province ; Khouzestan Province ; Iran
    Repository Name: AquaDocs
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    Format: 19-28
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  • 9
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    Investigation on genetic structure of Russian sturgeon (Acipenser gueldenstaedtii) populations of the north (Volga River) and south Caspian Sea (coasts of Iran and Turkmenistan) using microsatellite techniques (2021)
    In:  http://aquaticcommons.org/id/eprint/23680 | 18721 | 2018-07-18 06:38:41 | 23680 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: A total of 28 specimens of adult Russian sturgeon brood fish from the Volga River (Astrakhan, Russia) and 42 specimens from the south Caspian Sea (coastline of Iran and Turkmenistan) were collected. About 2g of fin tissue was stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel electrophoresis and spectrophotometry. Polymerase Chain Reaction (PCR) was conducted using eight pairs of microsatellite primers and its products were electrophoresed using 6% polyacrylamide gel followed by silver nitrate staining. Allele sizes were measured in all populations, then genetic parameters were calculated using Gen Alex program and the phylogenetic relationship was determined and drawn using TFPGA program. A minimum of 10 and a maximum of 21 alleles were identified per locus and the observed heterozygosity ranged between 0.50-0.96 and the expected heterozygosity was 0.74-0.90 with an average of 0.68. Hardy-Weinberg equilibrium was observed at Ls-19, Ls-39 loci, but showed disequilibrium in other loci. FST index between Volga and South Caspian Sea samples was 0.031. The genetic similarity and distance was 0.661 and 0.414, respectively. Results of the present investigation indicate that there are no significant differences between the south Caspian Sea Russian sturgeon specimens.
    Keywords: Biology ; Population Genetics ; Genes ; Electrophoresis ; Fins ; DNA ; Genetic diversity ; Natural populations ; Phylogenetics ; Phylogeny ; Heterozygosity ; Migration ; Alcohols ; Spectrophotometry ; Genomics ; Silver nitrate ; Genetic structure ; Rivers ; Microsatellites ; Gel electrophoresis ; Polymerase chain reaction ; Primers ; Coasts ; Acipenser gueldenstaedtii ; Acipenser ; Brackish ; Caspian Sea ; Caspian Sea Turkmenistan ; Iran
    Repository Name: AquaDocs
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    Format: 69-80
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  • 10
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    Cryopreservation of carp spermatozoa by different extenders (2021)
    In:  http://aquaticcommons.org/id/eprint/24308 | 18721 | 2018-08-04 09:00:27 | 24308 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Production and storage of the best gametes are practices undertaken by fishery managers and researchers to improve fish reproduction technology and obtain the best spawners. The male spawners of carp (Cyprinus carpio) were chosen from two warm-water fish hatcheries near Rasht, Guilan Province. For the study, duration of motility of sperms, sperm density and fertilization rate were considered. The best samples were chosen for mropreservation with four different extenders and were checked by fertilization tests after seven days of preservation in liquid nitrogen. Results showed that by 1:1 dilution rate, the Alsever solution had the best results. The fertilization rate, production of eyed ego and larvae by this extender in a multistage cooling experiment were 45r, 47.81" and 22.63 respectively.
    Keywords: Biology ; Carp ; Cryopreservation ; Sperm ; Guilan Province ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 17-30
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