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  • 1
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    Effects of stocking density on growth performance and survival of three male morphotypes in all-male culture of Macrobrachium rosenbergii (De Man) (2018)
    Details
    Publication Date: 2021-05-19
    Description: A study was conducted to appraise the effect of different stocking densities on three male morphotypes named blue claw (BC), orange claw (OC) and small male (SM) at harvest in all-male culture and to assess the growth performance of BC, OC and SM in each isolated culture. Trials involving three stocking densities of all-male prawn viz., 20, 30 and 40 juvenile m-2 were carried out in replicates. After 4 months of culture, BC, OC and SM were sorted from all tanks and restocked at 5 m-2 in treatments BC, OC and SM respectively for 80 days. A difference in prawn density significantly (p〈0.05) affected adversely on morphotypes. The highest survival rate combined with good yield performance was from 20 juvenile m-2 stocking density with 21% BC, 62.5% OC and 16.5% SM, respectively. In isolation culture, the average specific growth rate of the SM population (1.22) was significantly higher than that of the OC (1.01) and the BC (0.43) population. The survival of the SM population was 100% while for others it was 72%. Absolute weight of prawn was significantly greater in the male OC (23.87 g) than the SM (19.57 g) and the BC males (6.31 g). Impacts of isolated culture on population structure were much more pronounced in the SM population than others.
    Description: Published
    Keywords: Aquaculture ; Freshwater prawn ; Macrobrachium rosenbergii ; Heterogeneous individual growth ; Male morphotypes ; Stocking density ; Isolation culture
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.738-750
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  • 2
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    A simple and rapid method for blood collection from walking catfish, Clarias batrachus (Linneaus, 1758) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Blood is collected from experimental animals for a wide range of scientific purposes including; hematology, clinical biochemistry parameters, immunology, studies in bacteriology, parasitology and investigations in reproductive performance and health. The number of methods employed to collect blood from fish include; the puncture of caudal vein, dorsal aorta or cardiac vessels and the severance of the caudal vein. Unfortunately, all these procedures are practically found to be slow and stressful to Clarias batrachus, including the popular caudal vein approach, likely due to the small size of caudal veins relative to the size of the species. In line with the universal ethical recommendations for taking blood from small research animals, we propose an alternative one-operator approach for C. batrachus that is simple, rapid and without the need to sacrifice the fish as with other methods. This procedure targets the dorsal aorta (a relatively larger blood vessel) in a sedated fish, punctured by inserting a needle directly from the anterior part of the anal fin about 2-5 mm behind the genital papilla, to draw the desired amount of blood. The technique is a one-operator procedure not requiring the help of an assistant or any special equipment to restrain the fish. The operation of the protocol is unique since it permits the continuous collection of blood from the same experimental fish over a varied time course and reduces the need for a large number of replicate animals. The advantages of the proposed protocol are also highlighted and discussed in detail.
    Description: Published
    Keywords: Haematology ; Catfish ; Aquaculture ; Blood sampling ; Clarias batrachus
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.935-944
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  • 3
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    A simple and rapid method for blood collection from walking catfish, Clarias batrachus (Linneaus, 1758) (2021)
    In:  http://aquaticcommons.org/id/eprint/23130 | 18721 | 2018-06-18 17:16:30 | 23130 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-13
    Description: Blood is collected from experimental animals for a wide range of scientific purposes including; hematology, clinical biochemistry parameters, immunology, studies in bacteriology, parasitology and investigations in reproductive performance and health. The number of methods employed to collect blood from fish include; the puncture of caudal vein, dorsal aorta or cardiac vessels and the severance of the caudal vein. Unfortunately, all these procedures are practically found to be slow and stressful to Clarias batrachus, including the popular caudal vein approach, likely due to the small size of caudal veins relative to the size of the species. In line with the universal ethical recommendations for taking blood from small research animals, we propose an alternative one-operator approach for C. batrachus that is simple, rapid and without the need to sacrifice the fish as with other methods. This procedure targets the dorsal aorta (a relatively larger blood vessel) in a sedated fish, punctured by inserting a needle directly from the anterior part of the anal fin about 2-5 mm behind the genital papilla, to draw the desired amount of blood. The technique is a one-operator procedure not requiring the help of an assistant or any special equipment to restrain the fish. The operation of the protocol is unique since it permits the continuous collection of blood from the same experimental fish over a varied time course and reduces the need for a large number of replicate animals. The advantages of the proposed protocol are also highlighted and discussed in detail.
    Keywords: Biology ; Fisheries ; Haematology ; Catfish ; Aquaculture ; Blood sampling ; Clarias batrachus ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 935-944
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  • 4
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    Feeding characteristics of Neogobius caspius in the south west coastline of the Caspian Sea (Gilan Province) (2021)
    In:  http://aquaticcommons.org/id/eprint/22360 | 18721 | 2018-03-27 22:34:32 | 22360 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-04
    Description: Neogobius caspius is an endemic species of the Caspian Sea which plays an important role in food chain as a predatory fish. The main aim of this study was to investigate selected feeding characteristics of Neogobius caspius in the south west coasts of the Caspian Sea. Monthly sampling was carried out using a bottom trawler at three stations (Astara, Anzali and Chabuksar) in three depths (0-5, 5-10 and 10-15m) on a monthly basis from October 2004 to September 2005. Relative gut length (RLG) was less than one suggesting that this species is carnivorous. RLG was significantly lower in older fish but gut length was longer (P〈0.05). Intensity of fullness was below the favorite degree (IF〈400) from October to March, however, it was higher (400〈IF〈900) from April to October at all stations, indicating that feeding conditions for this species is suitable in the study area. Study showed that N. caspius mainly fed on molluscs (Food preference, FP=100%), worms (FP=89%) and crustaceans (FP=74%), hence, this species is considered as euryphagus species.
    Description: Article includes abstract in Farsi on last page.
    Keywords: Biology ; Ecology ; Fisheries ; Neogobius caspius ; Feeding characteristics ; Relative gut length ; Food preference ; Caspian Sea ; Gilan Province ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 127-140
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  • 5
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    A putative probiotic isolated from hatchery reared juvenile Penaeus monodon (2021)
    In:  http://aquaticcommons.org/id/eprint/22560 | 18721 | 2018-04-26 18:42:51 | 22560 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-07
    Description: Probiotic is considered as a remedy to solve disease problem in shrimp aquaculture. Shrimp body and environment bacterial flora are recommended as reliable source for extracting of beneficial bacteria. Therefore, 5000 pieces of post larvae of Penaeus monodon were distributed equally in 4 tanks in probiotic and antibiotic free condition up to juvenile. Then microbiological samples from digestive system, muscles, body surface, rearing water and tank sediment were inoculated onto the selective media plates. Incident of total bacteria, Vibrio, Pseudomonas and Entrobacteriaceae were evaluated in different body parts and rearing environment of shrimp. The highest bacteria count for total bacteria, Vibrio, Pseudomonas was occurred in sediment followed by digestive system. No Entrobacteriaceae was observed from all samples. Isolated bacteria were identified and placed in 7 genera consisted of Vibrio, Shewanella, Burkholderia, Clavibacter, Staphylococcus, Brevibacterium, Corynebacterium, while one genus remained unidentified. Genus Shewanella exhibited highest frequent bacteria in digestive system of P. monodon which may be indicated its ability to colonization in digestive system. Shewanella algae was selected from whole isolated bacteria as candidate probiotic via well plate diffusion technique and cross streak antagonistic test against Vibrio harveyi, V. parahaemolyticus and V. alginolyticus. It was able to digest lipid and protein in tributyrin agar and skim milk agar.
    Keywords: Aquaculture ; Biology ; Fisheries ; Probiotic ; Shewanella algae ; Penaeus monodon ; Shrimp ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 849-866
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  • 6
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    Evaluation of selected substrates for collection of hatchery reared black-lip pearl oyster (Pinctada margaritifera) spat (2021)
    In:  http://aquaticcommons.org/id/eprint/22414 | 18721 | 2018-04-05 23:14:44 | 22414 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: An experiment was conducted to investigate the effect of collector materials and position on Pinctada margaritifera spat attachment. Settlement on polyethylene pipes (54.6%) was significantly higher (P 〈 0.05) than on plastic baskets (25%). Significantly higher (P 〈 0.05) spat catch was recorded from the collectors which were installed on lower height of settlement tank than upper. The number of dead spat after settlement on pipe (1.4%) and basket (1.5%) were not significantly different (P 〉 0.05). Possible factors causing the pattern of settlement reveals that it could be related to the material used in collector, reaction to light and gravity force. These results indicated that polyethylene pipes positioned horizontally in the third bottom part of the tank are suitable for the settlement of P. margaritifera pediveliger larvae in a hatchery and may also be suitable for grow out in the ocean.
    Description: Irregularity in pagination of volume 10, number 2: page range of this article (pp. 198-207) overlaps with page range of previous article (pp. 188-202).
    Keywords: Aquaculture ; Biology ; Fisheries ; Pinctada margaritifera ; Settlement ; Spat ; Collector ; Evaluation ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 198-207
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  • 7
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    Effects of stocking density on growth performance and survival of three male morphotypes in all-male culture of Macrobrachium rosenbergii (De Man) (2021)
    In:  http://aquaticcommons.org/id/eprint/22907 | 18721 | 2018-06-06 01:06:56 | 22907 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-11
    Description: A study was conducted to appraise the effect of different stocking densities on three male morphotypes named blue claw (BC), orange claw (OC) and small male (SM) at harvest in all-male culture and to assess the growth performance of BC, OC and SM in each isolated culture. Trials involving three stocking densities of all-male prawn viz., 20, 30 and 40 juvenile m^-2 were carried out in replicates. After 4 months of culture, BC, OC and SM were sorted from all tanks and restocked at 5 m^-2 in treatments BC, OC and SM respectively for 80 days. A difference in prawn density significantly (p〈0.05) affected adversely on morphotypes. The highest survival rate combined with good yield performance was from 20 juvenile m^-2 stocking density with 21% BC, 62.5% OC and 16.5% SM, respectively. In isolation culture, the average specific growth rate of the SM population (1.22) was significantly higher than that of the OC (1.01) and the BC (0.43) population. The survival of the SM population was 100% while for others it was 72%. Absolute weight of prawn was significantly greater in the male OC (23.87 g) than the SM (19.57 g) and the BC males (6.31 g). Impacts of isolated culture on population structure were much more pronounced in the SM population than others.
    Keywords: Aquaculture ; Biology ; Fisheries ; Freshwater prawn ; Macrobrachium rosenbergii ; Heterogeneous individual growth ; Male morphotypes ; Stocking density ; Isolation culture ; aquaculture ; Malaysia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 738-750
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