Publication Date:
1987-07-10
Description:
A wide variety of human tumors contain an amplified or overexpressed erbB-2 gene, which encodes a growth factor receptor-like protein. When erbB-2 complementary DNA was expressed in NIH/3T3 cells under the control of the SV40 promoter, the gene lacked transforming activity despite expression of detectable levels of the erbB-2 protein. A further five- to tenfold increase in its expression under influence of the long terminal repeat of Moloney murine leukemia virus was associated with activation of erbB-2 as a potent oncogene. The high levels of the erbB-2 product associated with malignant transformation of NIH/3T3 cells were observed in human mammary tumor cells that overexpressed this gene. These findings demonstrate a new mechanism for acquisition of oncogenic properties by genes encoding growth factor receptor-like proteins and provide a functional basis for the role of their overexpression in the development of human malignancies.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Di Fiore, P P -- Pierce, J H -- Kraus, M H -- Segatto, O -- King, C R -- Aaronson, S A -- New York, N.Y. -- Science. 1987 Jul 10;237(4811):178-82.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2885917" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Breast Neoplasms/genetics
;
Cell Line
;
*Cell Transformation, Neoplastic/genetics
;
DNA/genetics
;
Fibroblasts/*metabolism
;
Gene Expression Regulation
;
Genes, Viral
;
Humans
;
Mice
;
Moloney murine leukemia virus/genetics
;
Promoter Regions, Genetic
;
Proto-Oncogene Proteins/biosynthesis/genetics/*physiology
;
Rats
;
Receptor, Epidermal Growth Factor
;
Receptor, ErbB-2
;
Receptors, Cell Surface/genetics
;
Recombinant Fusion Proteins/biosynthesis/genetics/physiology
;
Simian virus 40/genetics
;
Tumor Stem Cell Assay
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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