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  • Agrobacterium tumefaciens C58  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Cloning and analysis of Agrobacterium tumefaciens C58 loci involved in glutamine biosynthesis: Neither the glnA (GSI) nor the glnII (GSII) gene plays a special role in virulence (1988)
    Springer
    Molecular genetics and genomics 212 (1988), S. 38-47 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium tumefaciens C58 ; Glutamine synthetase ; Glutamine auxotroph ; Virulence ; Nitrogen assimilation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using heterologous complementation of a glutamine synthetase deficient (glnA; GS-) Escherichia coli mutant strain and heterologous DNA hybridization probes from Rhizobium meliloti and Bradyrhizobium japonicum, three distinct Agrobacterium tumefaciens loci involved in glutamine biosynthesis were identified. These loci correspond to the glnA (GSI), glnII (GSII) and a third previously unidentified locus, which is capable of complementing an E. coli glnA mutant, but may be cryptic in A. tumefaciens. The gene products encoded by the cloned glnA and glnII loci were identified using maxicells. Single insertion mutations in the glnA (GSI) and glnII (GSII) genes and a glnA glnII double mutant were constructed using gene replacement techniques. These mutant strains were examined for GSI and II activities, for growth on a variety of nitrogen (N) sources and for virulence properties on Kalanchoë plants. Neither glnA (GSI) nor glnII (GSII) were found to be essential for tumour induction on Kalanchoë nor for opine catabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00322442
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  • 2
    Electronic Resource
    Electronic Resource
    The ntrC gene of Agrobacterium tumefaciens C58 controls glutamine synthetase (GSII) activity, growth on nitrate and chromosomal but not Ti-encoded arginine catabolism pathways (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 419-426 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium tumefaciens C58 ; Nitrogen regulation (ntr) ; Opine and arginine catabolism ; Glutamine synthetase ; Tn5 mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ntrC locus of Agrobacterium tumefaciens C58 has been cloned using the Azorhizobium sesbaniae ORSS571 ntrC gene as a DNA hybridization probe. Transposon Tn5 mutagenesis of the cloned ntrC locus was carried out and one Tn5 insertion within the region of highest DNA homology with A. sesbaniae ORS571 ntrC was used for gene replacement of the wild-type C58 ntrC gene. The A. tumefaciens ntrC::Tn5 mutant was found to be unable to grow on nitrate as sole nitrogen (N) source, to lack glutamine synthetase (GSII) activity and to be unable to use arginine (or ornithine) as sole N source, unless the Ti-encoded arginine catabolism pathway was induced with small amounts of nopaline. Thus the A. tumefaciens ntrC regulatory gene is essential for (transcriptional) activation of the GSII and nitrate reductase genes, as well as for the chromosomal but not the Ti-borne arginine catabolism pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331144
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