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  • ASTROPHYSICS  (1,586)
  • Life and Medical Sciences  (1,377)
  • 1
    Electronic Resource
    Electronic Resource
    Antibodies to sperm surface fertilization antigen (FA-1): Their specificities and site of interaction with sperm in male genital tract (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 26 (1990), S. 175-183 
    Details
    ISSN: 1040-452X
    Keywords: Sperm antigen ; Testis ; Blood-testis ; barrier ; Antisperm antibodies ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The fertiization antigen (FA-1) isolated from murine testes demonstrated its dimeric form of 49,000 ± 2,000 molecular weight (M.W.) or a monomer of 23,000 M.W. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The FA-1 was immunogenic in all three female rabbits tested and raised a high-titer antisera [enzyme-linked immunosorbent assay (ELISA) titers; 1:1,024 to 1:4,096]. The rabbit anti-FA-1 antisera predominantly recognized the dimeric form of 49,000 ± 2,000 M.W. on the Western blot of lithium diiodosalicylate (LIS)-solubilized murine testes. None of the antisera reacted with any somatic tissue, indicating germ-cell specificity of FA-1. To determine the cellular localization of the immunoreactive FA-1, a novel ultrasensitive immunogold-silver staining (IGSS) procedure was developed. The anti-FA-1-lgG showed intense staining in the luminal region of the seminiferous tubules containing spermatids and spermatozoa. No reaction was observed in the peripheral area of the tubules containing Sertoli cells, spermatogonia, leptotene, and zygotene spermatocytes. The biodistribution studies of 125I-labeled anti-FA-1 lgG in mice revealed that the antibodies do not bind to somatic tissues such as blood cell, liver, heart, kidney, muscle, and gastrointestinal tissue and do not transudate into testes nad seminal vesicle. However, the antibodies preferentially transudate into epididymis (especially corpus or cauda regions) and vas deferens to bind to sperm cells. In conclusion, our data indicate that FA-1 can induce an immune response that is germ cell-specific, directed against later stages of spermatogenesis. The antibodies to FA-1 interact with sperm after penetration through epididymis (especially corpus and cauda regions) and vas deferens rather than through testes and seminal vesicles.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080260212
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ulex europeus type I agglutinin detects carcinoembryonic antigen in extracts of human colorectal carcinoma (1988)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 37 (1988), S. 193-202 
    Details
    ISSN: 0730-2312
    Keywords: colorectal carcinoma ; Carcinoembryonic antigen ; UEA ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Recent interest has focused on fucosylated epitopes expressed on human neoplasms. The plant lectin Ulex europus agglutinin, Type I (UEA) binds fucosylated oligosac-charides, while UEA-reactive substances have a tissue distribution similar to carci-noembryonic antigen (CEA). We sought to determine if UEA reacted with CEA in extracts of fresh primary and metastatic colorectal carcinomas and paired normal tissues. The extracts were electrophoretically transferred to nitrocellulose membranes after the proteins were separated by SDS-PAGE in 10% polyacrylamide gels. The transfer membranes were then stained with peroxidase-conjugated UEA (UEA-P) or antibody to CEA (CEA-P). UEA-P reacted with a 170-190-kDa band in extracts of 22 of 30 primary tumors, 10 of 12 metastases, but only 1 of 5 villous adenomas. UEA-P generally did not react with normal colon or liver extracts. UEA-P also did not bind to 170-190-kDa molecules in Western transfers of a breast carcinoma metastatic to bowel and a focal nodular hyperplasia of liver. CEA-P displayed similar reactivity and detected CEA in a tumor extract negative for UEA. Fucose blocked binding of UEA-P to Western transfers of tumor extracts. CEA-P reacted with a 170-190-kDa substance in tumor extracts eluted with fucose from a column of immobilized UEA. Thus, UEA reacts with fucosylated oligosaccharides on most, but not all, species of CEA and may be a useful adjunct to anti-CEA immunohis-tochemistry.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240370206
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  • 3
    Electronic Resource
    Electronic Resource
    Localization of proteins on viral nucleocapsids using immunoelectron microscopy (1985)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 2 (1985), S. 139-146 
    Details
    ISSN: 0741-0581
    Keywords: Immunoelectron microscopy ; colloidal gold ; localization of proteins ; Sendai virus nucleocapsid ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: We have described an indirect immunoelectron microscope method to localize individual proteins on viral nucleocapsids using monoclonal antibodies and colloidal gold-conjugated second antibodies. The procedure provides good binding and retention of antibodies, good resolution(〈 24nm), and negligible nonspecific binding of antibodies to the background. In addition, the method is compatible with both negative and positive staining, the two staining procedures commonly used to derive ultrastructural information on viral chromosomes. We have illustrated the procedure by localizing the NP (nucleoprotein, ≍ 2,600 copies) and P (polymerase-associated protein, ≍ 300 copies) proteins on the nucleocapsid of Sendai virus, a paramyxovirus.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060020205
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  • 4
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    A ROSAT observation of NGC 5548 (1993)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: We present a detailed spectral and temporal analysis of a long ROSAT PSPC pointing centered on the bright Seyfert 1 galaxy NGC 5548. The spectrum is particularly complex, with a two-component model favored, confirming the existence of the soft excess in this source. In addition, an absorption feature arising from highly ionized oxygen in the line of sight has been detected, consistent with an origin in the 'warm absorber' which has been found to be a common feature in Seyfert galaxies observed by Ginga. The energy and depth of the feature should allow us to constrain the density and geometry of the absorbing gas, which may lie close to the central continuum source. NGC 5548 was in a low X-ray state during the observation, brightening by a factor of about 2 on a time-scale of about 2 d. Time-resolved spectral analysis shows a change in the ratio of the hard and soft fluxes, consistent with reprocessing models.
    Keywords: ASTROPHYSICS
    Type: Royal Astronomical Society, Monthly Notices (ISSN 0035-8711); 260; 3; p. 504-512.
    Format: text
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  • 5
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    Broad Band X-Ray Telescope observations of NGC 4151 - Iron line diagnostics (1992)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: The Fe K-alpha emission line is a potentially powerful diagnostic tool in the X-ray spectra of AGNs, however, this feature has previously been observed with relatively poor spectral resolution. The Broad Band X-Ray Telescope has provided us with the first high-quality, medium-resolution X-ray spectral data. We are able to constrain the width of the 6.4 keV Fe K-alpha line in NGC 4151 to be less than 160 eV FWHM (7,500 km/s), in contrast with former measurements of the line width. Also, we have limited information on the shape of the line profile. In addition to the strong narrow Fe K-alpha feature, we detect weaker broad residuals between 5-8 keV.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 2 - Letters (ISSN 0004-637X); 401; 1; p. L11-L14.
    Format: text
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  • 6
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    Temperature and iron abundance variation of the gas in the Perseus cluster (1994)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: We present the first two-dimensional map of the temperature and iron abundance in the Perseus cluster. Analysis of spectra obtained using the Gas Imaging Spectrometer on ASCA shows nonaxisymmetric variations in both the temperature and iron abundance. Traveling west from the cluster center, the temperature increases to 9 keV at 20 min and then decreases rapidly to 5 keV at 40 min. There is a hot (greater than 10 keV) region to the northwest of the cluster center. The abundance is approximately constant over much of the surveyed region, but there is evidence for an increased abundance in the northwest hot area and a gradual decrease in a westerly direction.
    Keywords: ASTROPHYSICS
    Type: Astrophysical Journal, Part 2 - Letters (ISSN 0004-637X); 436; 1; p. L67-L70
    Format: text
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  • 7
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    Composition of comet Halley dust particles from Giotto observations (1986)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: Mass spectra of cometary dust particles measured by the PIA dust particle analyzer aboard the Giotto spacecraft show some unexpected and striking features. First, small particles below 10 to the -14th g are much more abundant than anticipated by models. Second, most of the particles are rich in light elements such as H, C, N, and O, suggesting the validity of models that describe the cometary dust as including organic material. Third, the light elements specifically seem to have a low ratio of mass to volume. Three examples of original mass spectra showing typical compositions are given; these have been measured, and are compared with a computer-simulated mass spectrum.
    Keywords: ASTROPHYSICS
    Type: Nature (ISSN 0028-0836); 321; 336
    Format: text
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  • 8
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    A disturbance of the ion tail of Comet Halley and the heliospheric structure as observed by Sakigake (1986)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: In order to study the interaction between the solar wind measured by Sakigake and ion tail disturbances of comet Halley, more than 500 photographs of the comet taken on the ground during this apparition are surveyed. The focus of the present study is the December 31, 1985, event, when various types of disturbances occurred, including an outstanding disconnection event (DE)-like knot. Analysis of the Sakigake/IMF data reveals that comet Halley did not encounter the heliospheric neutral sheet on that day, demanding a new explanation for the DE-like event, different from the Niedner-Brandt model. During this event the comet encountered a high-speed solar wind stream from a coronal hole tongue of the sun. The event can be explained by a dynamic pressure model, according to which the DE-like plasmoid was caused by a sudden increase in the dynamic pressure of the solar wind. A result of the simulation work by Ogino is found to support this interpretation.
    Keywords: ASTROPHYSICS
    Type: Geophysical Research Letters (ISSN 0094-8276); 13; 821-824
    Format: text
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  • 9
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    Structure and dynamics of the plasma tail of comet P/Halley. I - Knot event on December 31, 1985 (1987)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: On the basis of about 500 photographs of comet Halley taken by many observers, Saito et al. (1986) classified the detected disturbances of the plasma tail as outstanding rays, streamer, helix, kink, arcade, and disconnection event (DE). In this paper, the interaction of the solar wind with the plasma tail of Comet P/Halley is examined by using results of observations by the Sakigake spacecraft of the December 31, 1985 event, which included various disturbances and one DE-like knot. On the basis of twenty photographs taken on December 31 by Japanese astronomers, the dynamic pressure model proposed by Saito et al. (1986) is examined, and the mechnism of the knot event that appeared in the plasma tail of the comet on December 31, is explained.
    Keywords: ASTROPHYSICS
    Type: Astronomy and Astrophysics (ISSN 0004-6361); 187; 1-2,
    Format: text
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  • 10
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    Far-infrared observations of Circinus and NGC 4945 galaxies (1990)
    In:  CASI
    Details
    Publication Date: 2013-08-31
    Description: Circinus and NGC 4945 are two galaxies luminous in the infrared and are characterized by compact non thermal radio nuclei, deep silicate absorption features and unusually strong water vapor maser luminosities. Moorwood and Glass (1984) have observed these galaxies extensively in the 1 to 20 micron range. In the far-infrared, observations up to 100 microns are available from the Infrared Astronomy Satellite (IRAS). In order to study the cool dust component of these galaxies, researchers observed them at 150 microns using the Tata Institute of Fundamental Research (TIFR) 100 cm balloon-borne telescope. Here, they report observations along with deconvolved maps at 50 and 100 microns obtained from the Chopped Photometric Channel (CPC) on board IRAS.
    Keywords: ASTROPHYSICS
    Type: NASA, Ames Research Center, The Interstellar Medium in External Galaxies: Summaries of Contributed Papers; p 86-88
    Format: application/pdf
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