Publication Date:
2011-06-04
Description:
To construct sophisticated biochemical circuits from scratch, one needs to understand how simple the building blocks can be and how robustly such circuits can scale up. Using a simple DNA reaction mechanism based on a reversible strand displacement process, we experimentally demonstrated several digital logic circuits, culminating in a four-bit square-root circuit that comprises 130 DNA strands. These multilayer circuits include thresholding and catalysis within every logical operation to perform digital signal restoration, which enables fast and reliable function in large circuits with roughly constant switching time and linear signal propagation delays. The design naturally incorporates other crucial elements for large-scale circuitry, such as general debugging tools, parallel circuit preparation, and an abstraction hierarchy supported by an automated circuit compiler.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Qian, Lulu -- Winfree, Erik -- New York, N.Y. -- Science. 2011 Jun 3;332(6034):1196-201. doi: 10.1126/science.1200520.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Bioengineering, California Institute of Technology, Pasadena, CA 91125, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21636773" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Computer Simulation
;
*Computers, Molecular
;
DNA/*chemistry/*metabolism
;
DNA, Single-Stranded/*chemistry/*metabolism
;
Logic
;
Nucleic Acid Conformation
;
*Nucleic Acid Hybridization
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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