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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 22 (1996), S. 272-278 
    ISSN: 1432-0789
    Keywords: Key words Take all ; Manganese oxidation ; Virulence ; Wheat ; Gaeumannomyces graminis var. tritici ; Epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two successive experiments described here show that the Mn-oxidizing ability of isolates of the take-all fungus Gaeumannomyces graminis var. tritici is positively correlated with the isolate virulence. Isolates of G. graminis var. tritici were screened for virulence on wheat in soil culture pot tests and visually scored for Mn-oxidizing capacity on agar plates. A comparison between experiments showed that the Mn-oxidizing abilility of an isolate may be attenuated, as is virulence, with subculturing. Using a solution culture system, we calculated the rate of oxidation of Mn2+ by a virulent isolate, Ggt 500, as 4 nmol g–1 (dry weight hyphae) h–1. The significance of these results to the epidemiology of the take-all disease of wheat is discussed.
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  • 2
    ISSN: 1432-0789
    Keywords: Mn reduction ; Rhizosphere bacteria ; Gaeumannomyces graminis ; Antagonism ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five bacterial strains capable of Mn reduction were isolated from the rhizosphere of plants growing in different South Australian soils. They differed in their Mn-reducing capacity. The antagonism of these strains compared to the imported strain 2–79 (from the United States) against Gaeumannomyces graminis var. tritici was tested in agar and in a soil sandwich experiment at different Mn2+ concentrations in the soil. In addition, wheat seeds were coated with the different strains and with MnSO4 or with MnSO4 only in order to investigate their effect on plant growth and Mn uptake. With one exception, all strains inhibited the growth of G. graminis in agar, but to different degrees. In contrast, only two strains significantly inhibited the growth of the fungus in the soil. The hyphal density was decreased more than the hyphal length. The Mn2+ concentration in the soil also had a marked effect on fungal growth; low Mn concentrations slightly increased while high Mn concentrations strongly decreased the fungal growth. Seed treatment with MnSO4 only (+Mn) increased Mn uptake above that of the control (no seed treatment). Only the weakest Mn reducer on agar significantly increased plant growth and Mn uptake from soil in comparison with the Mn treatment. One strain was tested as seed coating without adding MnSO4; it increased the plant growth to an extent similar to the Mn treatment. Increasing the Mn uptake by plants may be one of the growth-promoting effects exerted by rhizosphere bacteria.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 22 (1996), S. 272-278 
    ISSN: 1432-0789
    Keywords: Take all ; Manganese oxidation ; Virulence ; Wheat ; Gaeumannomyces graminis var. tritici ; Epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two successive experiments described here show that the Mn-oxidizing ability of isolates of the take-all fungus Gaeumannomyces graminis var. tritici is positively correlated with the isolate virulence. Isolates of G. graminis var. tritici were screened for virulence on wheat in soil culture pot tests and visually scored for Mn-oxidizing capacity on agar plates. A comparison between experiments showed that the Mn-oxidizing ability of an isolate may be attenuated, as is virulence, with subculturing. Using a solution culture system, we calculated the rate of oxidation of Mn2+ by a virulent isolate, Ggt 500, as 4 nmol g-1 (dry weight hyphae) h-1. The significance of these results to the epidemiology of the take-all disease of wheat is discussed.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Irrigation science 3 (1981), S. 29-44 
    ISSN: 1432-1319
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The interrelations between nitrogen and water supply on the growth and yield of wheat (Triticum aestivum L.) were studied with particular attention to water use and grain yield in relation to the time of application of nitrogen to the plants. Nitrogen was applied at seeding or deferred until the double ridge-stage of development. Effects of both soil-applied and foliar-applied nitrogen were investigated under deficient or adequate water supply. The supply of water to the crop controlled the level of yield obtained and the response of the crop to applied N was markedly greater in drought-free conditions. Application of N resulted in increases in both crop leaf area duration and water use. Greater responses to N were obtained when the fertilizer was applied to the soil, rather than as a foliar spray.
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  • 5
    ISSN: 1432-1319
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat was grown in field and glasshouse experiments to assess the effect of nitrogen fertilizer on yield when water stress occurred in the later half of the growth. N application was deferred until the main culm apex of the plant was at the double-ridge stage of development. In the glasshouse water stress was imposed by altering the watering regime; in the field it was anticipated as naturally occurring and compared to an irrigated control. The response to deferred N was much stronger at adequate water supply giving rise to a significant positive N X W interaction effect. This positive N X W interaction was shown by number of ears, leaf area index, green area duration, water use and root growth, as well as grain yield. In both the glasshouse and field, N increased post-anthesis green area duration (PGD) which was highly correlated with grain yield, but since the components of grain yield determining the response to N were largely established by anthesis (number of ears), PGD does not appear to increase grain yield, which was rather caused by increased survival of tillers. In concert with its effect on PGD, deferred N resulted in greater root survival and/or growth at deeper layers late in the season. Water stress as measured in these experiments was insufficient to cause decreases in yield from use of N at low water supply. However, in the field nitrogen application did lower plant water potential late in the growing season.
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  • 6
    ISSN: 1432-1319
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In wheat plants grown at two levels of water supply and three levels of nitrogen supply, proline accumulation in flag leaves and ears was enhanced by low water potential. Nitrogen appeared to increase proline through its effects on water potential; in this respect there were marked differences between two cultivars under study. A considerable discontinuity existed within the ears of both wheat and barley in accumulation of proline under stress; while the maternal tissues accumulated high concentrations of proline, very little increase was found in grain. This partitioning occurred also in excised ears, and so was independent of the rest of the plant; nor was it dependent on in situ synthesis of the respective tissues alone, since exogenous L-proline was also distributed in the same way. Some exogenously supplied DL-proline however, did enter the grain. Grain proline content was not maintained at a low level due to proline incorporation into protein. The significance of these results is discussed in relation to the evidence that wheat plants under water stress nevertheless maintain a high water potential in the grain.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 101 (2000), S. 1100-1108 
    ISSN: 1432-2242
    Keywords: Key words Barley ; Manganese efficiency ; RFLP mapping ; Marker-assisted Selection ; Plant nutrition ; 4HS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In many cropping regions of the world, yield is limited by the availability of micronutrients, and micronutrient-efficient cultivars provide a yield advantage. Traditional methods of testing cultivars for micronutrient efficiency are time-consuming and laborious. Molecular markers linked to loci controlling micronutrient efficiency will allow more rapid and efficient selection and introgression of these traits than is currently possible. Using a pot-based bioassay and bulked segregant analysis of an F2 population, we have identified several RFLPs (grouped distally on chromosome 4HS) linked to a locus for manganese efficiency in barley. This manganese efficiency locus has been designated Mel1. Pot bioassay analysis of intercrosses suggests that three useful sources of manganese efficiency are likely to be allelic at the Mel1 locus. Field evaluation of marker selected F4 progeny supports the major role of Mel1 in the genetic control of manganese efficiency. Adoption of marker assisted selection for this trait in the Southern Australian barley breeding program has occurred. This has been facilitated by the demonstration that the Mel1 allele of Amagi Nijo can be distinguished from 95 other locally useful varieties and breeder’s lines on the basis of RFLPs identified by just two molecular markers.
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  • 8
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; genotypes ; interaction ; manganese ; oxidation ; take-all ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all is a world-wide root-rotting disease of cereals. The causal organism of take-all of wheat is the soil-borne fungus Gaeumannomyces graminis var tritici (Ggt). No resistance to take-all, worthy of inclusion in a plant breeding programme, has been discovered in wheat but the severity of take-all is increased in host plants whose tissues are deficient for manganese (Mn). Take-all of wheat will be decreased by all techniques which lift Mn concentrations in shoots and roots of Mn-deficient hosts to adequate levels. Wheat seedlings were grown in a Mn-deficient calcareous sand in small pots and inoculated with four field isolates of Ggt. Infection by three virulent isolates was increased under conditions which were Mn deficient for the wheat host but infection by a weakly virulent isolate, already low, was further decreased. Only the three virulent isolates caused visible oxidation of Mn in vitro. The sensitivity of Ggt isolates to manganous ions in vitro did not explain the extent of infection they caused on wheat hosts. In a similar experiment four Australian wheat genotypes were grown in the same Mn-deficient calcareous sand and inoculated with one virulent isolate of Ggt. Two genotypes were inefficient at taking up manganese and were very susceptible to take-all, one was very efficient at taking up manganese and was resistant to take-all, and the fourth genotype was intermediate for both characters. All genotypes were equally resistant under Mn-adequate conditions.
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  • 9
    ISSN: 1573-5036
    Keywords: basal stem ; nitrate ; Rhizoctonia solani ; root disease ; spring wheat ; tissue nutrient concentrations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root disease caused by Rhizoctonia solani is a common problem of spring wheat in South Australia. There are reports that nitrogen applications can reduce the incidence and severity of the disease. A glasshouse trail in pots examined the effects of disease and of applied nitrogen on wheat growth, and evaluated the utility of the basal stem nitrate concentration in diagnosing deficiency in plants with and without root disease. Plants were harvested at the mid-tillering stage. Shoot growth was increased by applied nitrogen until a maximum yield was attained, after which additional N had no effect on shoot yield. Root growth, however, responded positively only to low levels of applied N, after which it declined, and in the highest N treatment root mass was less than in the plants without applied N. Root disease caused severe reductions in plant growth, and both root and shoot mass were affected similarly. Even though growth of diseased plants responded positively to applied nitrogen the response was less than that of disease-free plants. The critical concentration of basal stem nitrate-N did not appear to be affected by root disease, and was estimated at 1200 mg kg-1, consistent with other glasshouse data. The basal stem nitrate-N concentration, either in fresh or dried tissue, appeared a better diagnostic tool of N stress than did total shoot N concentration or content, because of sharper definition of critical concentrations. Concentrations of other nutrients in shoot tissue were affected differentially by both applied nitrogen and root disease, but generally did not reach critical levels, although phosphorus and magnesium appeared deficient in very disease-stressed plants.
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  • 10
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; lignin ; manganese ; Mn-efficiency ; phenolics ; roots ; resistance mechanisms ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Differential resistance of four Triticum aestivum L. genotypes to isolates of take-all fungus (Gaeuman-nomyces graminis var. ritici Walker) was tested in a complete factorial experiment set up in a growth chamber using Mn-deficient Wangary sand amended with four rates of Mn. Mn-efficient cultivars produced more dry matter at low supply of Mn. Fertilization with Mn significantly increased its accumulation in roots and shoots. The most sensitive measure of take-all infection was the total length of root stellar lesions; these lesions were reduced by Mn fertilization and were shorter in Mn-efficient genotypes. The resistance-enhancing effect of Mn was the most obvious in the Mn-inefficient genotype (Bayonet) and the least obvious in the Mn-efficient one (C8MM). Phenolics biosynthesis in roots was clicited by fungal infection, especially in the case of the highly virulent isolate. The weakly virulent isolate increased phenolics concentration in roots much more if no Mn was added, indicating that the resistance-enhancing effect of Mn may not be directly exerted through the effects on phenolics biosynthesis. Lignin concentration in roots decreased due to Mn fertilization, while no effect of take-all infection was noted. It appears that biosynthesis of phenolics and lignin in wheat roots has a low Mn requirement which can be satisfied at environmental Mn concentrations below those necessary for optimum plant growth. ei]Section editor: A C Borstlap ei]Section editor: H Lambers
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