Summary
Five bacterial strains capable of Mn reduction were isolated from the rhizosphere of plants growing in different South Australian soils. They differed in their Mn-reducing capacity. The antagonism of these strains compared to the imported strain 2–79 (from the United States) against Gaeumannomyces graminis var. tritici was tested in agar and in a soil sandwich experiment at different Mn2+ concentrations in the soil. In addition, wheat seeds were coated with the different strains and with MnSO4 or with MnSO4 only in order to investigate their effect on plant growth and Mn uptake. With one exception, all strains inhibited the growth of G. graminis in agar, but to different degrees. In contrast, only two strains significantly inhibited the growth of the fungus in the soil. The hyphal density was decreased more than the hyphal length. The Mn2+ concentration in the soil also had a marked effect on fungal growth; low Mn concentrations slightly increased while high Mn concentrations strongly decreased the fungal growth. Seed treatment with MnSO4 only (+Mn) increased Mn uptake above that of the control (no seed treatment). Only the weakest Mn reducer on agar significantly increased plant growth and Mn uptake from soil in comparison with the Mn treatment. One strain was tested as seed coating without adding MnSO4; it increased the plant growth to an extent similar to the Mn treatment. Increasing the Mn uptake by plants may be one of the growth-promoting effects exerted by rhizosphere bacteria.
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References
Becker J, Cook RJ (1988) Role of siderophores in suppression of Pythium species and production of increased-growth response of wheat by fluorescent pseudomonads. Phytopathology 78:778–782
Brisbane PG, Rovira AG (1988) Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads. Plant Pathol 37:104–111
Brown PH, Graham RD, Nicholas DJD (1984) The effects of manganese and nitrate supply on the levels of phenols and lignin in young wheat plants. Plant and Soil 81:437–440
De Vrind JPM, Boogerd FC, De Vrind-De Jong EW (1988) Manganese reduction by a marine Bacillus species. J Bacteriol 167:30–34
De Weger LA, van Boxtel R, van der Burg B, Gruters RA, Geels FP, Schippers B (1986) Siderophores and outer membrane proteins of antagonistic, plant-growth-stimulating, root-colonizing Pseudomonas spp. J. Bacteriol 165:585–594
Geels FP, Schippers B (1983) Selection of antagonistic fluorescent Pseudomonas spp. and their root colonization and persistence following treatment of seed potatoes. Phytopathol Z 108:193–206
Ghiorse WC (1988) The biology of manganese transforming microorganisms in soils. In: Graham RD, Hannam RJ, Uren NC (eds) Manganese in soils and plants. Kluwer Academic Publishers, Dordrecht, pp 75–85
Ghiorse WC, Ehrlich HL (1976) Electron transport components of the MnO2 reductase system and the location of the terminal reductase in a marine bacillus. Appl Environ Microbiol 31:977–985
Graham RD (1983) The role of plant nutrition in resistance to pathogenic diseases with special reference to the trace elements. Adv Bot Res 10:221–276
Graham RD, Rovira AD (1984) A role for manganese in the resistance of wheat plants to take-all. Plant and Soil 78:441–444
Grose MJ, Parker Ca, Sivasithamparam K (1983) Growth of Gaeumannomyces graminis var. tritici in soil: Effects of temperature and water potential. Soil Biol Biochem 16:211–216
Howell CR, Stipanovic RD (1980) Suppression of Pythium ultimum-induced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, Pyoluteorin. Phytopathology 70:712–715
Huber DM, Wilhelm NS (1988) The role of manganese in disease resistance. In: Graham RD, Hannam RJ, Uren NC (eds) Manganese in soils and plants. Kluwer Academic Publishers, Dordrecht, pp 155–173
Kloepper JW, Schroth NM (1981a) Pseudomonas siderophores: A mechanism explaining disease suppressive soils. Phytopathology 71:232
Kloepper JW, Schroth MN (1981b) Relationship of in vitro antibiosis of plant-growth-promoting rhizobacteria to plant growth and the displacement of the root microflora. Phytophatology 71:1020–1024
Leeper GW (1947) The forms and reactions of manganese in the soil. Soil Sci 63:79–94
Loper JE (1988) Role of fluorescent siderophore production in biological control of Phytium ultimum by a Pseudomonas fluorescens strain. Phytopathology 78:166–172
Reis EM, Cook RJ, McNeal BL (1982) Effect of mineral nutrition on take-all of wheat. Phytopathology 72:224–229
Ridge EH, Rovira AD (1971) Phosphatase activity of intact young wheat roots under non-sterile conditions. New Phytol 70:1017–1020
Simon A, Sivasithamparam K, Macnish GC (1987) Biological suppression of the saprophytic growth of Gaeumannomyces graminis var. tritici in soil. Can J Microbiol 33:515–519
Sivasithamparam K, Parker CA (1978) Effects of certain isolates of bacteria and actinomycetes on Gaeumannomyces graminis var. tritici and take-all of wheat. Aust J Bot 26:773–782
Sparrow LA, Uren NC (1987) Oxidation and reduction of Mn in acidic soils: Effect of temperature and soil pH. Soil Biol Biochem 19:143–148
Trimble RB, Ehrlich HL (1968) Bacteriology of manganese nodules: III. Reduction of MnO2 by two strains of nodule bacteria. Appl Microbiol 16:695–702
Uren NC (1981) Chemical reduction of an insoluble higher oxide of manganese by plant roots. J Plant Nutr 4:65–71
Weller DM (1983) Colonization of wheat roots by a fluorescent pseudomonad suppressive to take-all. Phytopathology 73:1548–1553
Weller DM, Cook RJ (1981) Control of take-all of wheat with fluorescent pseudomonads. Phytopathology 71:1007
Weller DM, Cook RJ (1983) Suppression of take-all of wheat by seed treatments with fluorescent pseudomonads. Phytopath 73:463–467
Wilhelm NS, Graham RD, Rovira AD (1990) Control of Mn status and infection rate by genotype of both host and pathogen in the wheat take-all interaction. Plant and Soil 123:267–275
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Marschner, P., Ascher, J.S. & Graham, R.D. Effect of manganese-reducing rhizosphere bacteria on the growth of Gaeumannomyces graminis var. tritici and on manganese uptake by wheat (Triticum aestivum L.). Biol Fert Soils 12, 33–38 (1991). https://doi.org/10.1007/BF00369385
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DOI: https://doi.org/10.1007/BF00369385