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  • 1
    Electronic Resource
    Electronic Resource
    Genetic markers for manganese efficiency in durum wheat (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Manganese (Mn) deficiency is a major constraint of alkaline soils around the world, particularly for cultivation of durum wheat, which is more intolerant of low Mn levels than either common wheat or barley. Genetic variation for Mn efficiency exists in the current germplasm of durum wheat. Several restriction fragment length polymorphisms (RFLPs) previously shown to be linked to the Mel1 locus for Mn efficiency on chromosome 4HS of barley were tested on 88 selected F2 plants of the durum cross, ‘Stojocri 2’ (Mn efficient) בHazar’ (Mn inefficient). The Mel1-linked RFLP marker Xcdo583a was closely linked to the trait and explained over 42% of the total variation for Mn efficiency in the ‘Stojocri 2’/‘Hazar’ F2 progeny. This marker has the potential to provide a valuable tool for the marker-assisted selection of Mn-efficient durum progeny derived from crosses with ‘Stojocri 2’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.00690.x
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  • 2
    Electronic Resource
    Electronic Resource
    Variation at the Nor loci in triticale derived from tissue culture (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 637-643 
    Details
    ISSN: 1432-2242
    Keywords: Tissue culture ; Triticale ; Ribosomal DNA ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants derived from tissue cultures of six triticale genotypes were the subject of an analysis for changes in the rRNA genes located at the site of nucleolar organizer regions (the Nor loci) on chromosomes 1B, 6B and 1R. In addition whole plant phenotypes and the chromosomal constitutions of their progenies were examined for alterations. Following treatment of DNA with the restriction endonuclease Taq1, it was possible to assign electrophoretic bands representing rDNA spacer sequences to each of the chromosomes known to carry a major Nor locus. In general, the rRNA genes were found to be stable except in one family where a marked reduction in the number of rDNA units was observed. This reduction in 1R rDNA spacer sequences was heritable and correlated with reduced C-banding at the position of Nor-R1 on chromosome 1R. The change was clearly a consequence of tissue culture since six other plants regenerated from the same culture, and the original parent, did not carry the alteration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264268
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  • 3
    Electronic Resource
    Electronic Resource
    Somaclonal variation in wheat: genetic and cytogenetic characterisation of alcohol dehydrogenase 1 mutants (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 644-653 
    Details
    ISSN: 1432-2242
    Keywords: Somaclonal variation ; Alcohol dehydrogenase ; Cytogenetics ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The progeny of 551 regenerants of the hexaploid wheat cultivar ‘Millewa’ were analysed for somaclonal mutants at the threeAdh-1 loci in hexaploid wheat. Seventeen regenerants gave rise to progeny having altered ADH1 zymograms. Progeny with altered zymograms in 13 of these regenerants were aneuploid. The remaining 4 regenerants gave rise to euploid progeny with altered ADH1 zymograms. The genetics of three of these somaclonal mutants is described in detail. These regenerants were interpreted to possess a 4Aα isochromosome, a 3BS/4Aα translocation and a 7BS/4Aα translocation, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289003
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  • 4
    Electronic Resource
    Electronic Resource
    RFLP markers associated with Sr22 and recombination between chromosome 7A of bread wheat and the diploid species Triticum boeoticum (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 1039-1045 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; Sr22 ; Triticum aestivum ; T. boeoticum ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of the bread wheat variety Schomburgk, and related lines in its pedigree, identified RFLP markers associated with the segment of chromosome 7A carrying the Sr22 gene derived from the diploid species T. boeoticum. The distribution of the RFLP markers indicated that at least 50% of 7AS and 80% of 7AL in Schomburgk is of T. boeoticum origin. Evaluation of five sets of nearisogenic lines, backcross lines in 20 different genetic backgrounds and an F2 population segregating for Sr22 demonstrated a very low level of recombination between the 7A chromosomes of T. boeoticum and T. aestivum. Several recombinants carrying Sr22 but with a much reduced segment of T. boeoticum were identified and these may prove useful in the breeding of further varieties with Sr22.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224536
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  • 5
    Electronic Resource
    Electronic Resource
    Mapping and validation of chromosome regions conferring boron toxicity tolerance in wheat (Triticum aestivum) (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 767-777 
    Details
    ISSN: 1432-2242
    Keywords: Key words Boron toxicity ; Boron tolerance ; Mapping ; Wheat ; Marker-assisted selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Boron is an essential plant micro-nutrient which can be phytotoxic to plants if present in soils in high concentration. Boron toxicity has been recognised as an important problem limiting production in the low rainfall areas of southern Australia, West Asia and North Africa. Genetic variation for boron toxicity tolerance in wheat has been well-characterised. The efficiency of breeding for boron toxicity tolerance could be greatly enhanced by the development of molecular markers associated with QTLs for tolerance in wheat. A population of 161 doubled haploids from a cross between the tolerant cultivar Halberd and the moderately sensitive cultivar Cranbrook was used to identify chromosomal regions involved in boron tolerance. A combined RFLP and AFLP linkage map of the Cranbrook x Halberd population was used to identify chromosomal regions involved in the boron tolerance traits measured. Regions on chromosome 7B and 7D were associated with leaf symptom expression. The region on chromosome 7B was also associated with the control of boron uptake and with a reduction in the effect of boron toxicity on root-growth suppression. RFLP markers at the chromosome 7B and 7D loci were shown to be effective in selecting for improved boron tolerance in an alternative genetic background. Halberd alleles at the chromosome 7B locus were associated with the concentration of boron in whole shoots and grain. The concentration of boron in whole shoots and in grain were both related to grain yield in a field trial conducted on soil containing toxic levels of boron. Implications relating to marker-assisted selection for boron toxicity tolerance in wheat are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051542
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  • 6
    Electronic Resource
    Electronic Resource
    RFLP mapping of manganese efficiency in barley (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 1100-1108 
    Details
    ISSN: 1432-2242
    Keywords: Key words Barley ; Manganese efficiency ; RFLP mapping ; Marker-assisted Selection ; Plant nutrition ; 4HS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In many cropping regions of the world, yield is limited by the availability of micronutrients, and micronutrient-efficient cultivars provide a yield advantage. Traditional methods of testing cultivars for micronutrient efficiency are time-consuming and laborious. Molecular markers linked to loci controlling micronutrient efficiency will allow more rapid and efficient selection and introgression of these traits than is currently possible. Using a pot-based bioassay and bulked segregant analysis of an F2 population, we have identified several RFLPs (grouped distally on chromosome 4HS) linked to a locus for manganese efficiency in barley. This manganese efficiency locus has been designated Mel1. Pot bioassay analysis of intercrosses suggests that three useful sources of manganese efficiency are likely to be allelic at the Mel1 locus. Field evaluation of marker selected F4 progeny supports the major role of Mel1 in the genetic control of manganese efficiency. Adoption of marker assisted selection for this trait in the Southern Australian barley breeding program has occurred. This has been facilitated by the demonstration that the Mel1 allele of Amagi Nijo can be distinguished from 95 other locally useful varieties and breeder’s lines on the basis of RFLPs identified by just two molecular markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051585
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