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  • 1
    ISSN: 1420-9071
    Keywords: Key words. Sterol carrier protein 2; protein expression; protein structure; nuclear magnetic resonance; N-terminal leader peptide.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The preform of the rabbit sterol carrier protein 2 (pre-rSCP2) was cloned, the uniformly 15N-labelled protein expressed in Escherichia coli and studied by three-dimensional 15N-resolved nuclear magnetic resonance spectroscopy. In spite of its low solubility in aqueous solution of only ∼0.3 mM, sequential 15N and 1H backbone resonance assignments were obtained for 105 out of the 143 residues. From comparison of the sequential and medium-range nuclear Overhauser effects (NOEs) in the two proteins, all regular secondary structures previously determined in mature human SCP2 (hSCP2) [Szyperski et al. (1993) FEBS Lett. 335: 18–26] were also identified in pre-rSCP2. Near-identity of the backbone 15N and 1H chemical shifts and 1 : 1 correspondence of 24 long-range NOEs to backbone amide groups in the two proteins show that the residues 21 – 143 adopt the same globular fold in pre-rSCP2 and mature hSCP2. The N-terminal 20-residue leader peptide of pre-rSCP2 is flexibly disordered in solution and does not observably affect the conformation of the polypeptide segment 21 – 143.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Electrical engineering 76 (1993), S. 273-283 
    ISSN: 1432-0487
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Description / Table of Contents: Contents Elliptical discs of cold rolled transformer steel sheets show interesting torque characteristics under application of homogenous magnetic fields. Discs of certain axis relations and orientations with respect to the rolling direction could be used in rotors of small synchronous motors, as they show torque characteristics of a symmetric four-pole magnet. Discs with other certain geometric properties allow to sensibly examine the magnetic quality of the sheet by simple torsion magnetometry. If the magnetic field is strong enough to saturate the specimen in the direction of the field, the torque characteristic can be calculated by superposition of magnetocrystalline-anisotropy and shape-anisotropy. The spatial distribution of the energy is depicted as three dimensional area. The directions of minimum energy indicate the possibilities for stable magnetic states. A sequence of these states of equilibrium supplies the torque characteristic.
    Notes: Übersicht Elliptische Scheiben aus anisotropen FeSi-Blech mit Gosstextur zeigen je nach Orientierung der elliptischen Hauptachsen bezüglich der Walzrichtung sehr unterschiedliche Drehmomentverläufe im starken Magnetfeld. In ausgewählten Fällen könnten mit solchen Scheiben zwei- oder vierpolige Rotoren kleiner Synchronmotoren für frequenzgesteuerte Antriebe realisiert werden. Bei einer Ausrichtung der längeren Ellipsoidachse in einem Winkel von 55° zur Walzrichtung reagiert der Drehmomentverlauf besonders empfindlich auf eine eventuelle geringe Fehlorientierung der Kristallite in der Blechebene. Daraus könnte sich ein einfaches Verfahren zur Qualitätskontrolle von Elektroblechen ergeben. Ist das Magnetfeld stark genug, um die Probe in jeder beliebigen Richtung in der Blechebene zu sättigen, dann kann der Drehmomentverlauf aus der Summe von Kristall- und Formanisotropieenergie ermittelt werden. Die räumliche Verteilung dieser berechneten Gesamtenergie wird in Form von Energieflächen veranschaulicht, und die daraus resultierenden Drehmomentverläufe werden Messungen an kornorientierten Transformatorblech-Scheiben unterschiedlicher Ellipsoidformen und- ausrichtungen gegenübergestellt.
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  • 3
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Recombinant fusion proteins offer important new therapeutic approaches for the future. This report describes the use of three different genetic strategies (i.e. “mono-”, “bi-” and “tri-cistronic” vectors) to achieve stable secretion from BHK cells of a glycosylated antibody-cytokine fusion protein designed for use in anti-tumour therapy. It describes selection of a robust and effective production cell line based on stability of secretion of the product, quality of mRNA and protein products and performance in in vitro bioassays for potency. The data obtained at this stage were utilised in the selection of a suitable candidate production cell line. The relative productivity and general performance of the cells in stirred tank and fixed bed culture systems indicated that a variety of cell culture technologies provided robust tools for production of a highly selected cell clone. Consistency of the product glycosylation was determined by analysis of released oligosaccharides using matrix-assisted laser desorption ionisation – time of flight mass spectrometry and high-performance anion exchange chromatography. These investigations showed consistent expression of three glycoforms of the fusion protein which varied in their relative proportions in different culture systems and at different time points in a fixed bed reactor with continuous perfusion. In conclusion, this study dealt with a range of important scientific and technical issues which are essential for regulatory approval and commercial success of a recombinant protein and elucidates some useful markers for process development for similar recombinant biologicals.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 96 (1965), S. 1103-1104 
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 279 (1979), S. 536-538 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] After incorporation of [l-14C]palmitic acid into Butyrivibrio S2 the long-chain 14C-labelled hydrophobia moieties produced by methanolysis of the complex bacterial lipids (Table 1) consisted of: (1) about 7% cetyl alcohol; (2) some 40% of the dimethyl ester of a new type of long-chain dicarboxylic ...
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 239 (1972), S. 342-344 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Electronmicrographs of negatively stained (potassium phosphotungstate, /?H 7) specimens of (a) unsonicated egg lecithin dispersion, (b) egg lecithin vesicles and (c) egg lecithin vesicles after adding alamethicin at a concentration of 10~3 M (molar ratio egg lecithin to alamethicin=9 :1). ...
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 261 (1976), S. 390-394 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The conformation of the polar headgroup of phosphatidylcholine in bilayer vesicles has been investigated using paramagnetic probes. The extended disposition of the polar group when bound to cations is maintained when anions are ...
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 254 (1975), S. 154-156 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The procedures used to obtain the surface pressure (n)-molecular area (A) isotherms for chromatographically pure 1,2-dipalmitoyl phosphatidylcholine (lecithin) have been described before7'8. The preparation and adsorption characteristics of l-14C-acetyl-|3-casein A have also been published9-10: ...
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  • 9
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 224 (1969), S. 692-694 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Alamethicin is a cyclic polypeptide of slightly variable composition containing the amino-acids (GluN)2, (Glu)1, (Pro)2, (Gly)1, (Ala)2, (2-Methyl Ala)7 or 8, (Val)2, (Leu)1-. The amino-acid sequence has now been determined by B. S. Hartley and J. Payne (personal communication). The average ...
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  • 10
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Fructose-1,6-bisphosphatase ; Glucose repression ; Gene activation ; Gluconeogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis and the FBP1 gene is not transcribed during growth with glucose. Genetic analysis indicated a positive regulation of FBP1 expression after exhaustion of glucose. By linker-deletion analysis, two upstream activation sites (UAS1 and UAS2) were localized and the respective UAS-binding factors (DAP I and DAP II for derepression activating protein) were identified by gel retardation. UAS1 and UAS2 span about 30 bp each, and are separated by approximately 30 bp. Both UAS sites act synergistically. Although UAS1 showed some similarities to the DNA-binding consensus for the general yeast activator Rap1, competition experiments and DEAE-chromatography proved that DAP I and Rap1 correspond to different proteins. Gel retardation by DAP I depended on carbon sources and did not occur in cells growing logarithmically with glucose, whereas a strong retardation signal was obtained with ethanol-grown cells. The present results suggest that DAP I and DAP II are the final regulatory elements for glucose derepression.
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