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  • Springer  (3)
  • 1990-1994  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Improvement of regeneration of Lycopersicon pennellii protoplasts by decreasing ethylene production (1991)
    Springer
    Plant cell reports 9 (1991), S. 539-543 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Lycopersicon pennellii shoots, cultured in vitro for more than a year (type I plants) produced few viable protoplasts in contrast to shoots cultured in vitro for less than five months (type II plants). Ethylene production of both plant types was compared. The low viability of plant type I protoplasts could be correlated with high ethylene production and an increased cell sap osmolality. The ethylene action inhibitor silver thiosulphate improved protoplast yield and viability, especially when using donor tissue, germinated and cultured on medium containing silver thiosulphate (type III plants). Moreover, the choice of cell wall degrading enzymes influenced protoplast viability, since ethylene release was significantly lower using Cellulase R 10 than Cellulysin. All improvements together resulted in an efficient protocol for the isolation and regeneration of Lycopersicon pennellii protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232327
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular genetic characterisation of the Asc locus of tomato conferring resistance to the fungal pathogen Alternaria alternata f. sp. lycopersici (1994)
    Springer
    Euphytica 79 (1994), S. 205-217 
    Details
    ISSN: 1573-5060
    Keywords: AAL-toxins ; Alternaria alternata ; Asc locus ; Lycopersicon esculentum ; positional cloning ; transposon tagging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Alternaria stem canker disease of tomato is caused by the fungal pathogen Alternaria alternata f. sp. lycopersici and its host-selective AAL-toxins. Resistance to the pathogen and insensitivity to the toxins are conferred by the Asc locus on chromosome 3L. Sensitivity to AAL-toxins is a relative character; the toxins inhibit development of all tested tomato tissues but susceptible cultivars are much more sensitive than resistant cultivars. In addition to tomato, some other plant and animal species are sensitive to the toxins as well. The likely mode of action of AAL-toxins is interference with sphingolipid biosynthesis by specific inhibition of ceramide synthase activity. To molecularly isolate Asc, transposon tagging and positional cloning strategies are applied. As a first step, transposon insertions and restriction fragment length polymorphism (RFLP) markers are identified in proximity of the Asc locus. Subsequently, the transposons are used to inactivate Asc by insertion mutagenesis, and the RFLP markers are used to identify yeast artificial chromosomes (YACs) with tomato DNA inserts. Once an Asc-insertion mutant and/or a YAC encompassing Asc has been obtained, physical isolation and characterisation of Asc will be conceivable. Elucidation of the molecular role of Asc will illuminate the specificity of host recognition by Alternaria alternata f. sp. lycopersici.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022521
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  • 3
    Electronic Resource
    Electronic Resource
    Induction of streptomycin resistance in the wild tomato Lycopersicon peruvianum (1990)
    Springer
    Molecular genetics and genomics 220 (1990), S. 261-268 
    Details
    ISSN: 1617-4623
    Keywords: Lycopersicon peruvianum ; Streptomycin resistance ; Mutagen NMU ; Protoplasts ; Chloroplast marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A protoplast mutagenesis and cell selection system was used for the isolation of streptomycin resistant Lycopersicon peruvianum colonies. Protoplasts were treated with the mutagen N-nitroso-methylurea and could be regenerated into fertile plants, carrying the streptomycin resistant character. Several classes of streptomycin resistance could be distinguished. Reciprocal crosses between streptomycin resistant and sensitive plants showed a non-Mendelian transmission of the resistance trait. Streptomycin resistance is the first selectable and maternally inherited cell organelle marker described in tomato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260492
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