Summary
A protoplast mutagenesis and cell selection system was used for the isolation of streptomycin resistant Lycopersicon peruvianum colonies. Protoplasts were treated with the mutagen N-nitroso-methylurea and could be regenerated into fertile plants, carrying the streptomycin resistant character. Several classes of streptomycin resistance could be distinguished. Reciprocal crosses between streptomycin resistant and sensitive plants showed a non-Mendelian transmission of the resistance trait. Streptomycin resistance is the first selectable and maternally inherited cell organelle marker described in tomato.
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Communicated by R. Hagemann
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Jansen, C.E., Snel, E.A.M., Akerboom, M.J.E. et al. Induction of streptomycin resistance in the wild tomato Lycopersicon peruvianum . Molec. Gen. Genet. 220, 261–268 (1990). https://doi.org/10.1007/BF00260492
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DOI: https://doi.org/10.1007/BF00260492