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Improvement of regeneration of Lycopersicon pennellii protoplasts by decreasing ethylene production

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Summary

Lycopersicon pennellii shoots, cultured in vitro for more than a year (type I plants) produced few viable protoplasts in contrast to shoots cultured in vitro for less than five months (type II plants). Ethylene production of both plant types was compared. The low viability of plant type I protoplasts could be correlated with high ethylene production and an increased cell sap osmolality. The ethylene action inhibitor silver thiosulphate improved protoplast yield and viability, especially when using donor tissue, germinated and cultured on medium containing silver thiosulphate (type III plants). Moreover, the choice of cell wall degrading enzymes influenced protoplast viability, since ethylene release was significantly lower using Cellulase R 10 than Cellulysin. All improvements together resulted in an efficient protocol for the isolation and regeneration of Lycopersicon pennellii protoplasts.

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Abbreviations

ACC:

1-Aminocyclopropane-1-carboxylic acid

FW:

Fresh Weight

Mes:

-Morpholino ethane sulphonic acid

NMU:

N-Nitroso-N-Methyl-Urea

PE:

Plating Efficiency = Number of calli / number of protoplasts x 100%

Pps:

protoplasts

STS:

Silver thiosulfate

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Author notes

  1. N. O. M. Rethmeier

    Present address: Plant Genetic Systems, Jozef Plateaustraat 22, B-9000, Gent, Belgium

Authors and Affiliations

  1. Department of Genetics, Free University, De Boelelaan, 1087, 1081, HV Amsterdam, The Netherlands

    N. O. M. Rethmeier, C. E. Jansen, E. A. M. Snel, H. J. J. Nijkamp & J. Hille

Authors
  1. N. O. M. Rethmeier
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  2. C. E. Jansen
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  3. E. A. M. Snel
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  4. H. J. J. Nijkamp
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  5. J. Hille
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Communicated by H. Lörz

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Rethmeier, N.O.M., Jansen, C.E., Snel, E.A.M. et al. Improvement of regeneration of Lycopersicon pennellii protoplasts by decreasing ethylene production. Plant Cell Reports 9, 539–543 (1991). https://doi.org/10.1007/BF00232327

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  • DOI: https://doi.org/10.1007/BF00232327

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