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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1997), S. 96-101 
    ISSN: 1432-0789
    Keywords: Pyrites ; Pyrite oxidation ; Gypsum Alkali soil ; Reclamation ; Soluble sulfur Welland rice ; Wheat ; Thiobacillus thioxidans ; Thiobacillus ferrooxidans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We evaluated the effect of agricultural-grade (AG) pyrites (total sulfur 22%) varying in water-soluble sulfur (1–8%) and gypsum on the soil properties and yields of rice and wheat in alkali soils during the years 1993–1995 at the Gudha and Saraswati experimental farms at the Central Soil Salinity Research Institute, Karnal, India. Gypsum and pyrites were applied on the basis of gypsum requirement (GR) of the soils. Results showed that the efficiency of AG pyrites in decreasing soil pH and exchangeable sodium percentage (ESP) and increasing crop yields was dependent on their water-soluble sulfur content at the time of application to the field. Pyrites with 5.5% and 8% soluble sulfur were as effective as gypsum. The freshly mined pyrite (water-soluble S 1%) was found to be inefficient in reclaiming alkali soils. We also explored the possibility of increasing the water-soluble sulfur content of pyrite by optimizing its storage conditions. When pyrite (1% water-soluble S) was stored under moist conditions by sprinkling water over the bags under a rain shelter, there was an enrichment of indigenous iron- and sulfur-oxidizing bacteria of pyrite, and the water-soluble sulfur increased to 5% within a period of 6 months. However no such increase occurred when pyrite was stored dry. We conclude that the soluble sulfur content of pyrite increased during its storage under moist conditions and should be between 6% and 8% at the time of its application to the field.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1996), S. 96-101 
    ISSN: 1432-0789
    Keywords: Key words Pyrites ; Pyrite oxidation ; Gypsum ; Alkali soil ; Reclamation ; Soluble sulfur ; Wetland rice ; Wheat ; Thiobacillus thioxidans ; Thiobacillus ferrooxidans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We evaluated the effect of agricultural-grade (AG) pyrites (total sulfur 22%) varying in water-soluble sulfur (1–8%) and gypsum on the soil properties and yields of rice and wheat in alkali soils during the years 1993–1995 at the Gudha and Saraswati experimental farms at the Central Soil Salinity Research Institute, Karnal, India. Gypsum and pyrites were applied on the basis of gypsum requirement (GR) of the soils. Results showed that the efficiency of AG pyrites in decreasing soil pH and exchangeable sodium percentage (ESP) and increasing crop yields was dependent on their water-soluble sulfur content at the time of application to the field. Pyrites with 5.5% and 8% soluble sulfur were as effective as gypsum. The freshly mined pyrite (water-soluble S 1%) was found to be inefficient in reclaiming alkali soils. We also explored the possibility of increasing the water-soluble sulfur content of pyrite by optimizing its storage conditions. When pyrite (1% water-soluble S) was stored under moist conditions by sprinkling water over the bags under a rain shelter, there was an enrichment of indigenous iron- and sulfur-oxidizing bacteria of pyrite, and the water-soluble sulfur increased to 5% within a period of 6 months. However no such increase occurred when pyrite was stored dry. We conclude that the soluble sulfur content of pyrite increased during its storage under moist conditions and should be between 6% and 8% at the time of its application to the field.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 21 (1996), S. 309-313 
    ISSN: 1432-0789
    Keywords: Nodulation ; Competitiveness ; Rhizobium sp. (Cicer) ; lacZ fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We constructed lacZ fusions in Rhizobium sp. (Cicer) by random Tn5-lacZ mutagenesis. The lacZ+ fusants formed blue colonies on a Rhizobial minimal medium containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal). Rhizobium sp. (Cicer) fusant HSL-2 was identified in nodules and soil in a mixed population on the basis of the lacZ+ phenotype. Nodule occupancy of inoculated Rhizobium sp. (Cicer) HSL-2 (lacZ+) was assessed by directly streaking the nodule sap on X-gal plates. This method revealed differences between rhizobia carrying identical antibiotic markers. The rhizobial population in soil was estimated by direct plate counts using a medium containing X-gal. Introduction of lacZ into the Rhizobium sp. thus provided a simple and direct method for identifying strains from nodules and soil.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archive of applied mechanics 67 (1997), S. 158-166 
    ISSN: 1432-0681
    Keywords: Key words Shock and detonation waves ; compressible fluid ; nonlinear wave ; supersonic and hypersonic flows
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary A one-dimensional unsteady gas flow induced by an impulsive motion of a piston is studied by using a method of characteristics. The flow pattern in the nonisentropic flow region has been determined along particle paths emerging from the front shock into the region and along the positive characteristics emerging from the piston. The analytical solution obtained here enables us to determine the locations of the moving shock boundaries. It is found that the solution, which is also numerically computed and graphically presented, provides a good beginning toward the exact description of shock dynamics.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 18 (1998), S. 59-63 
    ISSN: 1432-203X
    Keywords: Key words Cotton ; Gossypium hirsutum ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Selections were made among individual plants of Gossypium hirsutum cv `Coker 310' for high-frequency in vitro regeneration by somatic embryogenesis. After three generations of selection, a pure line for high-frequency somatic embryogenesis was selected and named Coker 310 FR (FR, fully regenerating). Coker 310 FR could be regenerated by following previously published protocols (see Materials and methods) and a modified protocol developed in this study that reduced the time necessary for in vitro regeneration. Coker 310 FR was crossed with individual plants of major cotton cultivars grown in India, namely `MCU 5', `MCU 7', `Khandwa 2', `Bikaneri Nerma', `F 846' that have been shown to be recalcitrant to in vitro regeneration, to evaluate the regeneration potential of F1s. All the F1s showed regeneration by somatic embryogenesis. However, the F1 of G. barbadense×G. hirsutum Coker 310 FR did not regenerate.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 21 (1996), S. 309-313 
    ISSN: 1432-0789
    Keywords: Key words Nodulation ; Competitiveness ; Rhizobium sp. (Cicer) ; lacZ fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We constructed lacZ fusions in Rhizobium sp. (Cicer) by random Tn5-lacZ mutagenesis. The lacZ+ fusants formed blue colonies on a Rhizobial minimal medium containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal). Rhizobium sp. (Cicer) fusant HSL-2 was identified in nodules and soil in a mixed population on the basis of the lacZ+ phenotype. Nodule occupancy of inoculated Rhizobium sp. (Cicer) HSL-2 (lacZ+) was assessed by directly streaking the nodule sap on X-gal plates. This method revealed differences between rhizobia carrying identical antibiotic markers. The rhizobial population in soil was estimated by direct plate counts using a medium containing X-gal. Introduction of lacZ into the Rhizobium sp. thus provided a simple and direct method for identifying strains from nodules and soil.
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  • 7
    ISSN: 1572-901X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The kinetics of oxidation of N-methyl-p-aminophenol (metol) by H2O2 in the presence of trace levels of FeIII have been studied in an HClO4 medium. The reaction mechanism is complex and the catalytic role of FeIII in the sulphato form is indicated. The following rate law accounts for the experimental observations: $$ - d[H_2 O_2 ]/dt =$$ $$k_{un}^i + \frac{{K_1 K_2 [metol][H_2 O_2 ][Fe^{3 + } ](k_1 + k_2 [metol])}}{{1 + K_1 [metol] + K_1 K_2 [metol][H_2 O_2 ]}}$$ where k un i is the initial rate for the uncatalysed reaction. The equilibrium constants K 1 and K 2 are the formation constants for the complexes [Fe(metol)]3+ and [Fe-(metol)(H2O2)]3+, respectively. The catalytic role of FeIII is explained by considering a ternary complex in the transition state.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 14 (1995), S. 1691-1693 
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 9
    ISSN: 1432-2242
    Keywords: Key words Bread wheat ; Grain protein content ; Microsatellite ; STMS ; QTL analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  This study was undertaken with a view to tag gene(s) controlling grain protein content (GPC) using molecular markers in bread wheat. For this purpose, the genotype PH132 with high protein content (13.5%) was crossed with genotype WL711 with significantly lower protein content (9.7%), and 100 RILs were derived. These RILs showed normal distribution for protein content. The parental genotypes were analysed with 232 STMS primer pairs for detection of polymorphism. Of these, 167 primer pairs gave scorable amplification products, and 57 detected polymorphism between the parents. Using each of these 57 primer pairs, we carried out bulked segregant analysis on RILs representing the two extremes of the distribution. One primer pair for the locus wmc41 showed association with protein content. This was further confirmed through selective genotyping. The co-segregation data on the molecular marker (wmc41) and protein content on 100 RILs was analysed by means of a single-marker linear regression approach. Significant regression suggested linkage between wmc41 and a QTL (designated as QGpc.ccsu-2D.) for protein content. The results showed that this marker-linked QTL accounted for 18.73% of the variation for protein content between the parents. The marker has been located on chromosome arm 2DL using nulli-tetrasomic lines and two ditelocentric stocks for chromosome 2D.
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  • 10
    ISSN: 1432-2242
    Keywords: Chickpea ; Oligonucleotide fingerprinting ; Simple-sequence repeats ; Genetic diversity ; DNA polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The abundance and polymorphism of 38 different simple-sequence repeat motifs was studied in four accessions of cultivated chickpea (Cicer arietinum L.) by in-gel hybridization of synthetic oligonucleotides to genomic DNA digested with 14 different restriction enzymes. Among 38 probes tested, 35 yielded detectable hybridization signals. The abundance and level of polymorphism of the target sequences varied considerably. The probes fell into three broad categories: (1) probes yielding distinct, polymorphic banding patterns; (2) probes yielding distinct, monomorphic banding patterns, and (3) probes yielding blurred patterns, or diffused bands superimposed on a high in lane background. No obvious correlation existed between abundance, fingerprint quality, and the sequence characteristics of a particular motif. Digestion with methyl-sensitive enzymes revealed that simple-sequence motifs are enriched in highly methylated genomic regions. The high level of intraspecific polymorphism detected by oligonucleotide fingerprinting suggests the suitability of simple-sequence repeat probes as molecular markers for genome mapping.
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