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  • Springer  (73)
  • 1990-1994  (63)
  • 1965-1969  (6)
  • 1925-1929  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    The gene for the α-subunit of ATPase: a site of homologous recombination in plant mitochondrial DNA also functions in somatic hybrid cells (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 33-38 
    Details
    ISSN: 1432-2242
    Keywords: Homologous recombination ; ATPase α-subunit ; Protoplast fusion ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Segments of mitochondrial DNA (mtDNA) carrying the gene for the α-subunit of F1-ATPase (atpA) were detected by Southern hybridization with atpA from pea as probe. In the case of Nicotiana langsdorffii, we identified four fragments that are derived from combinations of two different 5′ and two different 3′ flanking regions of atpA. All four types share the coding region, suggesting that they result from homologous recombination in the coding region of atpA. By contrast, N. glauca generated only one analogous fragment, which indicated the existence of only a single type of atpA in N. glauca. In the case of somatic hybrids obtained by fusion between protoplasts from N. langsdorffii and N. glauca, analysis with EcoRI or HindIII detected three new fragments in addition to the parental fragments. These new fragments can be explained by homologous recombination within the coding region of atpA. Our results show that the coding region of atpA is involved not only in intragenomic homologous recombination but can also be involved in homologous recombination between two parental mitochondrial genomes of somatic hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223978
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  • 2
    Electronic Resource
    Electronic Resource
    Metal concentrations in pelagic seabirds from the North Pacific Ocean (1990)
    Springer
    Archives of environmental contamination and toxicology 19 (1990), S. 704-711 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Concentrations of four essential elements (Fe, Mn, Zn, and Cu) and two toxic metals (Cd and Hg) were measured in selected tissues of 19 pelagic seabird species collected in the North Pacific and neighboring waters. Essential metal concentrations were generally highest in the liver and less variable than toxic metals among species and also within each species. Fe concentrations in the muscle were higher in Alcidae than in the other families, whereas the opposite trend was found for Fe and Mn in the liver. Zn concentrations varied among species, depending on the Cd concentrations. On the other hand, toxic metal concentrations were highest in the liver or kidney and varied widely among species, greatly depending on differences in the diet among species. Extraordinarily high Hg concentrations were found in Black-footed Albatrosses,Diomedea nigripes, exceeding 300 μg/g wet weight in some, and seemed to be due to constraints on the elimination of Hg. Also, some geographical differences in Cd and Hg concentrations of the seabirds were observed. The concentrations of Cd and Hg reported here, however, seem to be natural rather than due to environmental pollution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01183988
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  • 3
    Electronic Resource
    Electronic Resource
    Efficient acetic acid production by repeated fed-batch fermentation using two fermentors (1991)
    Springer
    Applied microbiology and biotechnology 36 (1991), S. 295-299 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In acetic acid fermentation, the number of viable cells decrease as the acetic acid concentration increases to more than about 40 g/l, which means that the productivity attainable by conventional fed-batch and repeated fed-batch operations using one fermentor is limited. In this paper, based on a fed-batch experiment using Acetobacter aceti 2096, a mathematical model was developed. The optimization carried out showed the superiority of repeated fed-batch operation using two fermentors. The performance evaluation was made with respect to productivity and product concentration. It was shown to be attractive in practice to use multiple fermentors, in particular for high product concentrations. Experiments were then conducted to ascertain the simulation results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208144
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of intracellular 2,4-D concentration on plantlet regeneration of rice (Oryza sativa L.) callus (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 364-366 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Intracellular 2,4-dichlorophenoxyacetic acid (2,4-D) concentration profiles in rice (Oryza sativa L.) callus were measured for various subculture periods. 2,4-D was taken up into the callus by uphill transportation from the medium. The intracellular 2,4-D taken up was metabolized gradually during cultivation. The intracellular 2,4-D concentration markedly affected the redifferentiation process after subculture in such a way that the higher its concentration was, the more strongly the redifferentiation process was suppressed, and the lesser was the browning of the callus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00902743
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  • 5
    Electronic Resource
    Electronic Resource
    Extracellular production of human hepatitis B virus preS2 antigen as hybrid proteins with Bacillus subtilis α-amylases in high-salt-concentration media (1993)
    Springer
    Applied microbiology and biotechnology 40 (1993), S. 341-347 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract To produce PreS2 antigen of human hepatitis B virus extra-cellularly in Bacillus subtilis, it was fused with the COOH-termini of B. subtilis α-amylases of 522 (Amy+), 467 (Amy+) and 443 (Amy−) amino acids, Among them, α-amylase-A467, which has 467 amino acids, was a relatively stable carrier when the cells were cultured in Luria-Bertani (LB) medium. The α-amylase-A443-PreS2 hybrid protein (Amy−) was quickly degraded. The α-amylase-A522-PreS2 hybrid was most efficiently produced when a B. subtilis transformant of a protease-super-deficient mutant was cultured in the presence of 0.5 m sodium sulphate. The production of A522-PreS2 hybrid protein under such conditions reached 5–10 mg/l and was eight, and 200–500 times higher than those obtained by the transformants of an alkaline/neutral protease-deficient mutant of B. subtilis, and a wild-type strain, in LB medium, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00170390
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  • 6
    Electronic Resource
    Electronic Resource
    Nonlinear kinetics of threo-methylphenidate enantiomers in a patient with narcolepsy and in healthy volunteers (1993)
    Springer
    European journal of clinical pharmacology 44 (1993), S. 79-84 
    Details
    ISSN: 1432-1041
    Keywords: Methylphenidate enantiomers ; Narcoleptic patient ; oral administration ; healthy volunteers ; plasma concentration ; nonlinear kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary We have studied the pharmacokinetics of methylphenidate enantiomers after the oral administration of different doses of racemic methylphenidate to one patient with narcolepsy and to four healthy volunteers. The plasma concentrations of (+)-methylphenidate were much higher than those of (−)-methylphenidate after each dose in all subjects. In the patient the oral clearance (CL/f) of (+)-methylphenidate fell 3-fold and the area under the concentration-time curve (AUC) rose 7-fold when the dose was increased from 20 to 40 mg (from 0.27 to 0.53 mg·kg−1), in spite of the relatively constant terminal half-life of 2.6–2.7 h. Similar dose-dependency was also observed in the healthy volunteers in the dose range of 10–60 mg (0.12–0.77 mg·kg−1). The mean value of CL/f for the 40 mg dose was significantly lower than that for the 20 mg dose. The mean AUC of the (+)-isomer corrected to a dose of 10 mg increased significantly between the 20 mg and 40 mg doses. In the urine (+)- and (−)-ritalinic acid were excreted for 48 h after each dose as 32–37% and 34–40% of the dose respectively. The mean total recoveries (sum of enantiomers of methylphenidate and its metabolite, ritalinic acid) in the urine were relatively constant (63–78% of the doses), suggesting that the changes in AUC with dose may not be due to a change in the intestinal absorption of racemic methylphenidate. We conclude that the nonlinear kinetics of (+)-methylphenidate may be due to saturation of its presystemic elimination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315285
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  • 7
    Electronic Resource
    Electronic Resource
    Delayed disposition of adriamycin and its active metabolite in haemodialysis patients (1993)
    Springer
    European journal of clinical pharmacology 44 (1993), S. 301-302 
    Details
    ISSN: 1432-1041
    Keywords: Adriamycin ; Haemodialysis ; adriamycinol ; pharmacokinetics ; moment analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271378
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  • 8
    Electronic Resource
    Electronic Resource
    Rhythmic wavelets recorded from an in vitro preparation of mammalian retina (1969)
    Springer
    Cellular and molecular life sciences 25 (1969), S. 551-553 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Die rhythmischen Potentiale an der aufsteigenden Phase derb-Welle des von der In-vitro-Präparation der Warmblüternetzhaut abgeleiteten Elektroretinogramms wurden in vivo dargestellt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01900816
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  • 9
    Electronic Resource
    Electronic Resource
    Direct synthesis ofp-methoxybenzyl carbazate andp-methoxybenzyloxycarbonylamino acids usingp-methoxybenzyl chloroformate as reagent (1969)
    Springer
    Cellular and molecular life sciences 25 (1969), S. 576-578 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung P-Methoxybenzyloxycarbonylhydrazid undp-Methoxybenzyloxycarbonylaminosäuren konnten durch die Schotten-Baumannsche Reaktion mitp- Methoxybenzyloxycarbonylchlorid als Reagens direkt synthetisiert werden. Die Reaktionsbedingungen für die Darstellung des Reagens wurden verbessert und seine Stabilität wurde untersucht.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01896518
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  • 10
    Electronic Resource
    Electronic Resource
    Mechanochemical block copolymerization in heterogeneous systems of the solid poly(vinyl chloride) with styrene by ultrasonic irradiation (1994)
    Springer
    Polymer bulletin 33 (1994), S. 317-323 
    Details
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Summary The effect of several forms of suspension polymerized poly(vinyl chloride) particles on mechanochemical block copolymerization in the solid poly(vinyl chloride)-styrene-sodium dodecyl sulfate solutions has been studied by ultrasonic irradiation at 60 °C. The block copolymerization of styrene was initiated by free radicals produced from the poly(vinyl chloride) particles by ultrasonic waves. The rates of copolymerization increased with increasing the additional amount of the solid poly(vinyl chloride), the porosity, and the average diameter of the grain particles. In particular, the influence of the average diameter was much larger than that of the porosity. When the porosity and the average diameter were increased, the rates of decrease in the viscosity-average degree of polymerization of the degraded poly(vinyl chloride) were much increased. In addition, the changes in the composition of the block copolymer and homopolymers in the reaction products were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314269
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