ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract To produce PreS2 antigen of human hepatitis B virus extra-cellularly in Bacillus subtilis, it was fused with the COOH-termini of B. subtilis α-amylases of 522 (Amy+), 467 (Amy+) and 443 (Amy−) amino acids, Among them, α-amylase-A467, which has 467 amino acids, was a relatively stable carrier when the cells were cultured in Luria-Bertani (LB) medium. The α-amylase-A443-PreS2 hybrid protein (Amy−) was quickly degraded. The α-amylase-A522-PreS2 hybrid was most efficiently produced when a B. subtilis transformant of a protease-super-deficient mutant was cultured in the presence of 0.5 m sodium sulphate. The production of A522-PreS2 hybrid protein under such conditions reached 5–10 mg/l and was eight, and 200–500 times higher than those obtained by the transformants of an alkaline/neutral protease-deficient mutant of B. subtilis, and a wild-type strain, in LB medium, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00170390
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