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  • 1980-1984  (4)
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  • 1
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Brassinosteroid (BR) stimulation of auxin-induced ethylene production and the particular step at which BR acts to promote such synthesis were studied in mung bean (Vigna radiata L. Rwilcz cv. Berken) hypocotyl segments. Increasing concentrations of methionine alone and in combination with 3 μM BR and 10 μM IAA had a minimal effect on ethylene production. With increasing concentrations of 1-aminocyclopro-pane-1-carboxylic acid (ACC), however, ethylene production increased. BR or IAA further enhanced ethylene production with maximum rates occurring when these compounds were added together with ACC. The addition of 10 μM CoCl2 in conjunction with BR and/or IAA resulted in 85–97% inhibition of ethylene production. When 20 μM cycloheximide was used in conjunction with BR and/or IAA there was a complete inhibition of ethylene production. Total inhibition also resulted when 1.0 μM aminoethoxy-vinylglycine (AVG) was used in combination with BR and/or IAA. AVG alone had no effect on ACC conversion to ethylene.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 53 (1981), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Brassinolide (BR), a naturally-occurring-steroidal lactone from rape (Brassica napus L.) pollen, was compared with auxin for activity in a number of bioassay systems. Responses similar to IAA were elicited by BR in bioassays based upon bean hypocotyl hook opening, elongation of maize mesocotyl, pea epicotyl and azuki bean epicotyl sections, and fresh weight increase in Jerusalem artichoke (2,4-D used) and pea epicotyl sections. The azuki bean and dwarf pea epicotyl bioassays were much more responsive to BR than IAA (at 10 μM). Responses approximately two-fold greater in magnitude were elicited by IAA in the maize mesocotyl, bean hypocotyl hook and Jerusalem artichoke bioassays. Little or no response was elicited by BR (0.01 to μM) in the cress root or decapitated pea-lateral bud bioassays. A powerful synergism between BR and IAA was observed in the azuki bean, pea epicotyl and bean hypocotyl hook bioassays. Although, as previously reported, other steroidal substances are active in some of the bioassay systems tested, none compared with BR in magnitude and diversity of elicited responses.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 60 (1984), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Three cytokinins and their ribosides were tested in conjunction with brassinosteroid (BR) for their effects on ethylene production in etiolated mung bean (Vigna radiata L. Rwilcz cv. Berken) hypocotyl segments. When varying concentrations of BR were tried in combination with a fixed amount of kinetin (10 μM), there was an increase in ethylene production with increasing concentrations of BR up to 3 μM, and a decline beyond. A stimulation in ethylene production was observed with increasing concentrations of each of the cytokinins used. However, when applied in conjunction with 3 μM BR, all cytokinin concentrations produced similar stimulatory patterns. When 3 μM BR or 10 μM 6-benzylaminopurine (BAP) was applied in conjunction with increasing concentrations of IAA, there was a stimulation in ethylene production with increasing concentrations of IAA. However, 3 μM BR and 10 μM BAP together with varying concentrations of IAA failed to alter the level of ethylene production.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 53 (1981), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Brassinolide (BR), a naturally-occurring steroidal lactone was compared with GA3 and kinetin for activity in a number of bioassays. BR was highly active in elongation bioassays employing the dwarf pea epicotyl and etiolated bean hypocotyl that are responsive to GA3 but not IAA (at 0.01 to 10 μM). The elongation of the cucumber hypocotyl elicited by BR and GA3 was reduced far more than that elicited by IAA, following removal of the cotyledons. BR, like GA3 also inhibited betacyanin accumulation in Amaranthus seedlings and prevented adventitious root initiation in hypocotyls of mung bean, dwarf bean and cucumber. Application of BR promoted rather than retarded senescence of Rumex leaves.While almost half as effective as kinetin in promoting expansion of cucumber cotyledons, BR was ineffective in cytokinin bioassays involving expansion of dwarf pea epicotyl hooks, dark synthesis of betacyanin in Amaranthus and retardation of Xanthium leaf disc senescence. Unlike the case with IAA, BR does not interact synergistically with GA3. BR has proven active in promoting stem elongation in plant bioassays responsive to either IAA or GA3, or to both phytohormones. This effect, coupled to its effect on adventitious root formation, qualifies BR as a unique plant growth substance.
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