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  • 1980-1984  (2)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of solanum laciniatum (1983)
    Springer
    Plant cell reports 2 (1983), S. 205-208 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In leaf derived callus cultures of Solanum laciniatum Ait. both phenolics and solasodine concentrations increased when medium phosphate or nitrogen concs. were reduced to one-eighth or when sucrose concentration was increased from 3 to 4–8 %. Under these conditions growth was reduced and final FW:DW fell. Growth was inhibited by sucrose depletion and nitrogen supple -mentation. On additional nitrogen the concentrations of phenolics and protein significantly increased, FW:DW was reduced and solasodine concentration was unaffected. In seedling derived cultures phosphate depletion resulted in a significant increase in phenolics concentration, an inhibition of growth and a rise in solasodine concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270105
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  • 2
    Electronic Resource
    Electronic Resource
    Solasodine production in rapidly proliferating tissue cultures of Solanum laciniatum ait (1983)
    Springer
    Plant cell reports 2 (1983), S. 69-72 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus and suspension cultures, established from seedling and leaf explants of Solanum laciniatum Ait were analysed for solasodine using a spectrophotometric assay. Solasodine concentration in both types of culture ranged from 0.5 – 1.0 mg/g dry wt., with a small number of callus explants containing higher concentrations. There was no overall fall in concentration as a result of serial subculture, and in suspension cultures the level remained constant throughout a single passage. Solasodine concentration was enhanced by the induction of organogenesis in both primary leaf explants and callus. ABA, added at 0.04 mg 1−1, increased solasodine yield in callus cultures whilst CEPA, at concentrations of 10 mg 1−1 and higher, inhibited production. Dark grown callus contained significantly more solasodine than light grown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270168
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    Paper (German National Licenses)
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