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  • 1
    Electronic Resource
    Electronic Resource
    Studies on ethylene binding by cell-free preparations from cotyledons of Phaseolus vulgaris L.: subcellular localization (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 5 (1982), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Studies on the subcellular location of ethylene binding activity from developing cotyledons of Phaseolus vulgaris L. are described. Binding activity has been shown to be predominantly membrane bound. When separated by rate-zonal centrifugation more than 70% of this activity was of low sedimentation rate. The slowly sedimenting band of activity was further fractionated into three bands by isopycnic density gradient centrifugation. The three bands occur at sucrose densities of 1.125 g cm−3, 1.155 g cm−3 and 1.175 g cm−3, corresponding to the distribution of putative marker enzymes for the cell endomembrane system and to protein body membranes. Further circumstantial evidence was obtained by electron microscopy and sucrose step gradient centrifugation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1365-3040.ep11588010
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  • 2
    Electronic Resource
    Electronic Resource
    A study of the subcellular localisation of an ethylene binding site in developing cotyledons of Phaseolus vulgaris L. by high resolution autoradiography (1982)
    Springer
    Planta 154 (1982), S. 48-52 
    Details
    ISSN: 1432-2048
    Keywords: Cotyledon ; Endoplasmic reticulum ; Ethylene binding site ; Phaseolus ; Protein body membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Use was made of light microscopy and high resolution electron microscope autoradiography to determine the subcellular localisation of a binding site with a high affinity and specificity for ethylene in developing cotyledons of Phaseolus vulgaris L. The results indicate that the binding site is located on the endoplasmic reticulum and protein body membranes, confirming previous studies using cellular fractionation and marker enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385495
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of solanum laciniatum (1983)
    Springer
    Plant cell reports 2 (1983), S. 205-208 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In leaf derived callus cultures of Solanum laciniatum Ait. both phenolics and solasodine concentrations increased when medium phosphate or nitrogen concs. were reduced to one-eighth or when sucrose concentration was increased from 3 to 4–8 %. Under these conditions growth was reduced and final FW:DW fell. Growth was inhibited by sucrose depletion and nitrogen supple -mentation. On additional nitrogen the concentrations of phenolics and protein significantly increased, FW:DW was reduced and solasodine concentration was unaffected. In seedling derived cultures phosphate depletion resulted in a significant increase in phenolics concentration, an inhibition of growth and a rise in solasodine concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270105
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  • 4
    Electronic Resource
    Electronic Resource
    Studies on ethylene binding by cell-free preparations from cotyledons of Phaseolus vulgaris L. (1980)
    Springer
    Planta 148 (1980), S. 397-406 
    Details
    ISSN: 1432-2048
    Keywords: Ethylene binding ; Hormone binding ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The preparation is described of a cell-free system from developing cotyledons of Phaseolus vulgaris cv. Canadian Wonder which is capable of binding ethylene. The binding is saturable and the apparent dissociation constant for ethylene is 6.4·10-10 M in solution. The binding site is associated with subcellular particles and treatment with Triton X-100 results in substantial solubilisation of the activity. The kinetics of association and dissociation of the ligand and the binding site are described. The system is heat labile and binding activity is diminished by treatment with some proteolytic enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388129
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on ethylene binding by cell free preparations from cotyledons of Phaseolus vulgaris L. (1980)
    Springer
    Planta 148 (1980), S. 407-411 
    Details
    ISSN: 1432-2048
    Keywords: Ethylene binding ; Hormone binding ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Various protein reactive agents such as dithioerythritol, dithiothreitol, mercaptoethanol and p-chloromercuribenzoate inhibit binding of ethylene to cell free preparations of Phaseolus vulgaris L. The effect of the thiols is partially reversed by treatment with diamide; occupation of the binding site by ligand diminishes the inhibition caused by p-chloromercuribenzoate but not that caused by thiols. Growth regulators other than ethylene do not affect binding. Physiologically active structural analogues of ethylene competitively inhibit binding of the growth regulator and their relative effectiveness in the cell free system closely resembles that in developmental processes controlled ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388130
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  • 6
    Electronic Resource
    Electronic Resource
    DNA and histone content of immature tracheary elements from cultured artichoke explants (1977)
    Springer
    Planta 135 (1977), S. 213-216 
    Details
    ISSN: 1432-2048
    Keywords: DNA ; Helianthus ; Histones ; Tissue culture ; Tracheary elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Relative amounts of DNA and histone were determined by Feulgen microdensitometry and alkaline fast-green microdensitometry in differentiating tracheary elements in cultured explants of Jerusalem artichoke (Helianthus tuberosus L.) tubers. The absence of endopolyploidy in cultured artichoke tissue was confirmed, and the nuclei of tracheary elements were exclusively at the 2C level for both DNA and histones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384892
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  • 7
    Electronic Resource
    Electronic Resource
    Rapid differentiation of tracheary elements in cultured explants of Jerusalem artichoke (1977)
    Springer
    Planta 135 (1977), S. 207-212 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cytokinin ; Explants ; Helianthus ; Tuber (explants) ; Xylem differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract the culture of Jerusalem artichoke (Helianthus tuberosus L.) tuber explants on filter paper discs moistened with liquid medium resulted in rapid and consistent xylem differentiation. The number of tracheary elements increased in discrete steps, the first at 48 h with a second at 56–58 h, following partially synchronous mitoses at 20 and 30 h. Factors favouring xylem cell differentiation were optimum levels of both an auxin and a cytokinin, low medium nitrogen concentrations, small volumes of medium, and high culture temperatures. A cell counting method employing Feulgen-stained nuclei and suitable for quantifyings small numbers of immature tracheary elements is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384891
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  • 8
    Electronic Resource
    Electronic Resource
    The metabolism of ethylene to ethylene oxide in cultivars of Vicia faba: Relationship with waterlogging resistance (1982)
    Springer
    Plant growth regulation 1 (1982), S. 203-207 
    Details
    ISSN: 1573-5087
    Keywords: Vicia faba ; ethylene ; ethylene oxide ; waterlogging ; metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The ability of cultivars of Vicia faba L. to metabolize ethylene and the resistance of such cultivars to waterlogging was assessed. Those cultivars showing the highest resistance to waterlogging also had the ability to rapidly metabolize ethylene. It is suggested that a causal relationship may exist between the two processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036999
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of various growth regulators on growth in vitro of cherry shoot tips (1982)
    Springer
    Plant growth regulation 1 (1982), S. 209-216 
    Details
    ISSN: 1573-5087
    Keywords: growth-regulators ; cherry shoot tips ; propagation in vitro ; cryogenic storage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An investigation was undertaken to examine the effects of nine different growth regulators on growth in vitro of shoot cultures of the semi-dwarfing cherry rootstock Colt (Prunus avium × P. pseudocerasus). The effects of each supplement on shoot extension and proliferation and also leaf and callus production were noted, and it was found that BAP has the ability to proliferate shoots, IBA nullifies this effect and that kinetin, ABA, GA3 and ethylene inhibit the growth of colt cultures. Conditions were established which resulted in a) optimum shoot growth prior to subsequent rooting or grafting; b) maximum shoot proliferation for rapid clonal multiplication and c) minimum shoot growth. This study will form the basis of an investigation into germplasm conservation of temperate fruit trees by both cryogenic storage and minimal growth techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037000
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  • 10
    Electronic Resource
    Electronic Resource
    Rapid seed multiplication by planting into beds micro tubers and in vitro plants (1987)
    Springer
    Potato research 30 (1987), S. 117-120 
    Details
    ISSN: 1871-4528
    Keywords: nursery ; plant density ; tuber yield ; tuber number ; multiplication rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Micro tubers of 3 weight categories with means of 0.63 g, 1.25 g or 2.50 g and in vitro plants were planted in nursery beds at densities of 24 and 48 plants per m2. The average tuber numbers and tuber weights were, respectively, 38% and 17% higher at 48 than at 24 plants per m2. In vitro plants produced significantly more but smaller tubers than did plants grown from micro tubers. Plants grown from the larger micro tubers produced a similar number of tubers but larger ones than did plants grown from small micro tubers. Tuber yields and multiplication rates are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357690
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