Abstract
Callus and suspension cultures, established from seedling and leaf explants of Solanum laciniatum Ait were analysed for solasodine using a spectrophotometric assay. Solasodine concentration in both types of culture ranged from 0.5 – 1.0 mg/g dry wt., with a small number of callus explants containing higher concentrations. There was no overall fall in concentration as a result of serial subculture, and in suspension cultures the level remained constant throughout a single passage. Solasodine concentration was enhanced by the induction of organogenesis in both primary leaf explants and callus. ABA, added at 0.04 mg 1−1, increased solasodine yield in callus cultures whilst CEPA, at concentrations of 10 mg 1−1 and higher, inhibited production. Dark grown callus contained significantly more solasodine than light grown.
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Abbreviations
- ABA:
-
abscisic acid
- BAP:
-
6-benzylaminopurine
- CCC:
-
2-chloroethyl trimethyl ammonium chloride
- CEPA:
-
2-chloroethyl phosphonic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA3 :
-
gibberellic acid
- IBA:
-
indolebutyric acid
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Chandler, S., Dodds, J. Solasodine production in rapidly proliferating tissue cultures of Solanum laciniatum ait. Plant Cell Reports 2, 69–72 (1983). https://doi.org/10.1007/BF00270168
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DOI: https://doi.org/10.1007/BF00270168