Publication Date:
1989-12-08
Description:
A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Huse, W D -- Sastry, L -- Iverson, S A -- Kang, A S -- Alting-Mees, M -- Burton, D R -- Benkovic, S J -- Lerner, R A -- New York, N.Y. -- Science. 1989 Dec 8;246(4935):1275-81.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2531466" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Antibodies, Monoclonal/*biosynthesis/genetics
;
Antibody Specificity
;
Antigen-Antibody Reactions
;
Bacteriophage lambda/*genetics
;
Base Sequence
;
Cloning, Molecular/methods
;
Escherichia coli/genetics
;
Gene Amplification
;
Gene Library
;
*Genetic Vectors
;
Hemocyanin/analogs & derivatives/immunology
;
Immunoglobulin Fab Fragments/biosynthesis
;
Immunoglobulin Fragments/*biosynthesis/genetics
;
Mice
;
Molecular Sequence Data
;
Organophosphorus Compounds/immunology
;
Recombinant Proteins/biosynthesis/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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