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  • American Association for the Advancement of Science (AAAS)  (1,202)
  • 1985-1989  (1,202)
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  • 1
    Publication Date: 1988-02-12
    Description: Mesoderm induction in the amphibian embryo can be studied by exposing animal region explants (destined to become ectoderm) to appropriate stimuli and assaying the appearance of mesodermal products like alpha-actin messenger RNA. Transforming growth factor beta 2 (TGF-beta 2), but not TGF-beta 1, was active in alpha-actin induction, while addition of fibroblast growth factor had a small synergistic effect. Medium conditioned by Xenopus XTC cells (XTC-CM), known to have powerful mesoderm-inducing activity, was shown to contain TGF-beta-like activity as measured by a radioreceptor binding assay, colony formation in NRK cells, and growth inhibition in CCL64 cells. The activity of XTC-CM in mesoderm induction and in growth inhibition of CCL64 cells was inhibited partially by antibodies to TGF-beta 2 but not by antibodies to TGF-beta 1. Thus, a TGF-beta 2-like molecule may be involved in mesoderm induction.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rosa, F -- Roberts, A B -- Danielpour, D -- Dart, L L -- Sporn, M B -- Dawid, I B -- New York, N.Y. -- Science. 1988 Feb 12;239(4841 Pt 1):783-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3422517" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/genetics ; Amphibians/*embryology ; Animals ; Cell Division/drug effects ; Cell Line ; Embryo, Nonmammalian/physiology ; Growth Substances/*physiology ; Mesoderm/*physiology ; Peptides/pharmacology/*physiology ; RNA, Messenger/genetics ; Transforming Growth Factors ; Xenopus
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 1989-12-15
    Description: Voyager 2 images of Neptune reveal a windy planet characterized by bright clouds of methane ice suspended in an exceptionally clear atmosphere above a lower deck of hydrogen sulfide or ammonia ices. Neptune's atmosphere is dominated by a large anticyclonic storm system that has been named the Great Dark Spot (GDS). About the same size as Earth in extent, the GDS bears both many similarities and some differences to the Great Red Spot of Jupiter. Neptune's zonal wind profile is remarkably similar to that of Uranus. Neptune has three major rings at radii of 42,000, 53,000, and 63,000 kilometers. The outer ring contains three higher density arc-like segments that were apparently responsible for most of the ground-based occultation events observed during the current decade. Like the rings of Uranus, the Neptune rings are composed of very dark material; unlike that of Uranus, the Neptune system is very dusty. Six new regular satellites were found, with dark surfaces and radii ranging from 200 to 25 kilometers. All lie inside the orbit of Triton and the inner four are located within the ring system. Triton is seen to be a differentiated body, with a radius of 1350 kilometers and a density of 2.1 grams per cubic centimeter; it exhibits clear evidence of early episodes of surface melting. A now rigid crust of what is probably water ice is overlain with a brilliant coating of nitrogen frost, slightly darkened and reddened with organic polymer material. Streaks of organic polymer suggest seasonal winds strong enough to move particles of micrometer size or larger, once they become airborne. At least two active plumes were seen, carrying dark material 8 kilometers above the surface before being transported downstream by high level winds. The plumes may be driven by solar heating and the subsequent violent vaporization of subsurface nitrogen.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smith, B A -- Soderblom, L A -- Banfield, D -- Barnet, C -- Basilevsky, A T -- Beebe, R F -- Bollinger, K -- Boyce, J M -- Brahic, A -- Briggs, G A -- Brown, R H -- Chyba, C -- Collins, S A -- Colvin, T -- Cook, A F 2nd -- Crisp, D -- Croft, S K -- Cruikshank, D -- Cuzzi, J N -- Danielson, G E -- Davies, M E -- De Jong, E -- Dones, L -- Godfrey, D -- Goguen, J -- Grenier, I -- Haemmerle, V R -- Hammel, H -- Hansen, C J -- Helfenstein, C P -- Howell, C -- Hunt, G E -- Ingersoll, A P -- Johnson, T V -- Kargel, J -- Kirk, R -- Kuehn, D I -- Limaye, S -- Masursky, H -- McEwen, A -- Morrison, D -- Owen, T -- Owen, W -- Pollack, J B -- Porco, C C -- Rages, K -- Rogers, P -- Rudy, D -- Sagan, C -- Schwartz, J -- Shoemaker, E M -- Showalter, M -- Sicardy, B -- Simonelli, D -- Spencer, J -- Sromovsky, L A -- Stoker, C -- Strom, R G -- Suomi, V E -- Synott, S P -- Terrile, R J -- Thomas, P -- Thompson, W R -- Verbiscer, A -- Veverka, J -- New York, N.Y. -- Science. 1989 Dec 15;246(4936):1422-49.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17755997" target="_blank"〉PubMed〈/a〉
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  • 3
    Publication Date: 1986-07-04
    Description: Voyager 2 images of the southern hemisphere of Uranus indicate that submicrometersize haze particles and particles of a methane condensation cloud produce faint patterns in the atmosphere. The alignment of the cloud bands is similar to that of bands on Jupiter and Saturn, but the zonal winds are nearly opposite. At mid-latitudes (-70 degrees to -27 degrees ), where winds were measured, the atmosphere rotates faster than the magnetic field; however, the rotation rate of the atmosphere decreases toward the equator, so that the two probably corotate at about -20 degrees . Voyager images confirm the extremely low albedo of the ring particles. High phase angle images reveal on the order of 10(2) new ringlike features of very low optical depth and relatively high dust abundance interspersed within the main rings, as well as a broad, diffuse, low optical depth ring just inside the main rings system. Nine of the newly discovered small satellites (40 to 165 kilometers in diameter) orbit between the rings and Miranda; the tenth is within the ring system. Two of these small objects may gravitationally confine the e ring. Oberon and Umbriel have heavily cratered surfaces resembling the ancient cratered highlands of Earth's moon, although Umbriel is almost completely covered with uniform dark material, which perhaps indicates some ongoing process. Titania and Ariel show crater populations different from those on Oberon and Umbriel; these were probably generated by collisions with debris confined to their orbits. Titania and Ariel also show many extensional fault systems; Ariel shows strong evidence for the presence of extrusive material. About halfof Miranda's surface is relatively bland, old, cratered terrain. The remainder comprises three large regions of younger terrain, each rectangular to ovoid in plan, that display complex sets of parallel and intersecting scarps and ridges as well as numerous outcrops of bright and dark materials, perhaps suggesting some exotic composition.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smith, B A -- Soderblom, L A -- Beebe, R -- Bliss, D -- Boyce, J M -- Brahic, A -- Briggs, G A -- Brown, R H -- Collins, S A -- Cook, A F 2nd -- Croft, S K -- Cuzzi, J N -- Danielson, G E -- Davies, M E -- Dowling, T E -- Godfrey, D -- Hansen, C J -- Harris, C -- Hunt, G E -- Ingersoll, A P -- Johnson, T V -- Krauss, R J -- Masursky, H -- Morrison, D -- Owen, T -- Plescia, J B -- Pollack, J B -- Porco, C C -- Rages, K -- Sagan, C -- Shoemaker, E M -- Sromovsky, L A -- Stoker, C -- Strom, R G -- Suomi, V E -- Synnott, S P -- Terrile, R J -- Thomas, P -- Thompson, W R -- Veverka, J -- New York, N.Y. -- Science. 1986 Jul 4;233(4759):43-64.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17812889" target="_blank"〉PubMed〈/a〉
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  • 4
    Publication Date: 1988-06-03
    Description: The human immunodeficiency virus type 1 (HIV-1) uses the CD4 protein as a receptor for infection of susceptible cells. A candidate structure for the HIV-1 binding site on the CD4 protein was identified by epitope mapping with a family of eight functionally distinct CD4-specific monoclonal antibodies in conjunction with a panel of large CD4-derived synthetic peptides. All of the seven epitopes that were located reside within two immunoglobulin-like disulfide loops situated between residues 1 and 168 of the CD4 protein. The CD4-specific monoclonal antibody OKT4A, a potent inhibitor of HIV-1 binding, recognized a site between residues 32 and 47 on the CD4 protein. By analogy to other members of the immunoglobulin superfamily of proteins, this particular region has been predicted to exist as a protruding loop. A synthetic analog of this loop (residues 25 to 58) showed a concentration-dependent inhibition of HIV-1-induced cell fusion. It is proposed that a loop extending from residues 37 to 53 of the CD4 protein is a binding site for the AIDS virus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jameson, B A -- Rao, P E -- Kong, L I -- Hahn, B H -- Shaw, G M -- Hood, L E -- Kent, S B -- AI25784/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1988 Jun 3;240(4857):1335-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Biology, California Institute of Technology, Pasadena 91125.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2453925" target="_blank"〉PubMed〈/a〉
    Keywords: Antibodies, Monoclonal/immunology ; Binding, Competitive ; Cell Fusion ; Disulfides/metabolism ; Epitopes/immunology ; Fluorescent Antibody Technique ; HIV/immunology/*metabolism ; HIV Envelope Protein gp120 ; Humans ; Immunoglobulins ; Peptide Fragments/chemical synthesis/immunology ; Protein Conformation ; Radioimmunoassay ; Receptors, HIV ; Receptors, Virus/*immunology ; Retroviridae Proteins/immunology ; T-Lymphocytes, Helper-Inducer/immunology/*microbiology
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  • 5
    Publication Date: 1985-11-22
    Description: Modulation of the growth of human and murine cell lines in vitro by recombinant human tumor necrosis factor-alpha (rTNF-alpha) and recombinant human interferon-gamma (rIFN-gamma) was investigated. rTNF-alpha had cytostatic or cytolytic effects on only some tumor cell lines. When administered together with rIFN-gamma, rTNF-alpha showed enhanced antiproliferative effects on a subset of the cell lines tested. In contrast to its effects on sensitive tumor cells, rTNF-alpha augmented the growth of normal diploid fibroblasts. Variations in the proliferative response induced by rTNF-alpha were apparently not due to differences in either the number of binding sites per cell or their affinity for rTNF-alpha. These observations indicate that the effects of rTNF-alpha on cell growth are not limited to tumor cells, but rather that this protein may have a broad spectrum of activities in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sugarman, B J -- Aggarwal, B B -- Hass, P E -- Figari, I S -- Palladino, M A Jr -- Shepard, H M -- New York, N.Y. -- Science. 1985 Nov 22;230(4728):943-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3933111" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Division/*drug effects ; Cell Line ; Cell Survival/drug effects ; Cell Transformation, Neoplastic/pathology ; Drug Synergism ; Glycoproteins/*pharmacology ; Humans ; Interferon-gamma/pharmacology ; Mice ; Recombinant Proteins/*pharmacology ; Tumor Necrosis Factor-alpha
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  • 6
    Publication Date: 1985-01-04
    Description: Accumulation of very long chain fatty acids in X-linked and neonatal forms of adrenoleukodystrophy (ALD) appears to be a consequence of deficient peroxisomal oxidation of very long chain fatty acids. Peroxisomes were readily identified in liver biopsies taken from a patient having the X-linked disorder. However, in liver biopsies from a patient having neonatal-onset ALD, hepatocellular peroxisomes were greatly reduced in size and number, and sedimentable catalase was markedly diminished. The presence of increased concentrations of serum pipecolic acid and the bile acid intermediate, trihydroxycoprostanic acid, in the neonatal ALD patient are associated with a generalized diminution of peroxisomal activities that was not observed in the patient with X-linked ALD.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goldfischer, S -- Collins, J -- Rapin, I -- Coltoff-Schiller, B -- Chang, C H -- Nigro, M -- Black, V H -- Javitt, N B -- Moser, H W -- Lazarow, P B -- AG-01468/AG/NIA NIH HHS/ -- AM-17702/AM/NIADDK NIH HHS/ -- N5-03356/PHS HHS/ -- etc. -- New York, N.Y. -- Science. 1985 Jan 4;227(4682):67-70.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3964959" target="_blank"〉PubMed〈/a〉
    Keywords: Adrenoleukodystrophy/genetics/metabolism/*pathology ; Adult ; Animals ; Bile Acids and Salts/metabolism ; Catalase/metabolism ; Child ; Child, Preschool ; Diffuse Cerebral Sclerosis of Schilder/*pathology ; Female ; Humans ; Liver/pathology ; Male ; Microbodies/*pathology ; Oxidation-Reduction ; Pipecolic Acids/blood ; Rats ; *X Chromosome
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  • 7
    Publication Date: 1986-02-21
    Description: The first accelerator radiocarbon dates of rock varnishes are reported along with potassium/argon ages of lava flows and conventional radiocarbon dates of pluvial lake shorelines, in an empirical calibration of rock varnish K(+) + Ca(2+)/Ti(4+) ratios with age in the Mojave Desert, eastern California. This calibration was used to determine the cation-ratio dates of 167 artifacts. Although cation-ratio dating is an experimental method, some dates suggest human occupation of the Mojave Desert in the late Pleistocene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dorn, R I -- Bamforth, D B -- Cahill, T A -- Dohrenwend, J C -- Turrin, B D -- Donahue, D J -- Jull, A J -- Long, A -- Macko, M E -- Weil, E B -- Whitley, D S -- Zabel, T H -- New York, N.Y. -- Science. 1986 Feb 21;231(4740):830-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17774077" target="_blank"〉PubMed〈/a〉
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  • 8
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1988-11-25
    Description: The human immunodeficiency virus type 1 (HIV-1) shows extensive genetic variation and undergoes rapid evolution. The fidelity of purified HIV-1 reverse transcriptase was measured during DNA polymerization in vitro by means of three different assays. Reverse transcriptase from HIV-1 introduced base-substitution errors in DNA from the bacteriophage phi X174 amber3 at estimated frequencies of 1/2000 to 1/4000. Analyses of misincorporation rates opposite a single template adenine residue showed that HIV-1 reverse transcriptase catalyzed nucleotide mismatches with a specificity of A:C much greater than A:G greater than A:A. The high error rate of HIV-1 reverse transcriptase in vitro translates to approximately five to ten errors per HIV-1 genome per round of replication in vivo. This high error rate suggests that misincorporation by HIV-1 reverse transcriptase is, at least in part, responsible for the hypermutability of the AIDS virus. The specificity of misincorporation may provide a basis for the systematic construction of antiviral nucleosides.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Preston, B D -- Poiesz, B J -- Loeb, L A -- CA-07263-03/CA/NCI NIH HHS/ -- N01AI72654/AI/NIAID NIH HHS/ -- R35-CA-39903/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1988 Nov 25;242(4882):1168-71.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, University of Washington, Seattle 98195.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2460924" target="_blank"〉PubMed〈/a〉
    Keywords: Avian Myeloblastosis Virus/enzymology ; Bacteriophage phi X 174/genetics ; DNA/*biosynthesis ; DNA Polymerase II/metabolism ; DNA, Viral/biosynthesis ; Electrophoresis, Polyacrylamide Gel ; HIV/*enzymology/genetics ; Kinetics ; Moloney murine leukemia virus/enzymology ; Mutation ; Nucleotides/metabolism ; RNA-Directed DNA Polymerase/*metabolism
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  • 9
    Publication Date: 1988-02-05
    Description: Ground-based observations of the object IRAS 14348-1447, which was discovered with the Infrared Astronomical Satellite, show that it is an extremely luminous colliding galaxy system that emits more than 95 percent of its energy at far-infrared wavelengths. IRAS 14348-1447, which is receeding from the sun at 8 percent of the speed of light, has a bolometric luminosity more than 100 times larger than that of our galaxy, and is therefore as luminous as optical quasars. New optical, infrared, and spectroscopic measurements suggest that the dominant luminosity source is a dustenshrouded quasar. The fuel for the intense activity is an enormous supply of molecular gas. Carbon monoxide emission has been detected at a wavelength of 2.6 millimeters by means of a new, more sensitive receiver recently installed on the 12-meter telescope of the National Radio Astronomy Observatory. IRAS 14348-1447 is the most distant and luminous source of carbon monoxide line emission yet detected. The derived mass of interstellar molecular hydrogen is 6 x 10(10) solar masses. This value is approximately 20 times that of the molecular gas content of the Milky Way and is similar to the largest masses of atomic hydrogen found in galaxies. A large mass of molecular gas may be a prerequisite for the formation of quasars during strong galactic collisions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sanders, D B -- Scoville, N Z -- Soifer, B T -- New York, N.Y. -- Science. 1988 Feb 5;239(4840):625-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17759060" target="_blank"〉PubMed〈/a〉
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  • 10
    Publication Date: 1988-12-09
    Description: A diverse terrestial biota inhabited polar latitudes during the Cretacous, 105 to 130 Ma (million years ago), along what is now the southeast coast of Australia This biota, from rocks in the Otway and Strzelecki groups, cnsisted of more than 150 taxa of vertebrates, invertebrates, and plants. Oxygen isotope ratios in diagenetic calcite suggest that mean annual temperatures were most likely less than 5 degrees C, and rings present in the fossil araucarian-podocarp-ginko woods indicate saonality. Southeastern Austalia, thus, seems to have had a cool, seasonal, nontropical climate. Dinosaurs that have been recovered are up to five species and three genera of hypsilophodontids, all of which were endemic, and three species of theropods. The occurrence of Allosaurus sp. and labyrinthodont amphibians, which had become extinct elsewhere in the Jurassic, indicate that isolation may have allowed extended surival of these taxa in Australia. In that dinosaurs coped with high latitude for at least 65 million years [Valaginian to Albian time in Australia and Campanian to Maastrictian time (80 to 65 Ma) in Alaska] suggests that cold and darkness may not have been prime factors bringing about the extinction of dinosaurs and some other groups at the Cretaceous-Tertiary boundary, unless they were prolonged.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rich, P V -- Rich, T H -- Wagstaff, B E -- Mason, J M -- Douthitt, C B -- Gregory, R T -- Felton, E A -- New York, N.Y. -- Science. 1988 Dec 9;242(4884):1403-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17802135" target="_blank"〉PubMed〈/a〉
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