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  • 1995-1999  (141)
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Year
  • 1
    ISSN: 1432-072X
    Keywords: Key wordsMethylosulfonomonas methylovora ; Marinosulfonomonas methylotropha ; Methanesulfonic ; acid ; 16S rRNA phylogeny ; 16S rDNA sequences ; Proteobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two novel genera of restricted facultative methylotrophs are described; both Methylosulfonomonas and Marinosulfonomonas are unique in being able to grow on methanesulfonic acid as their sole source of carbon and energy. Five identical strains of Methylosulfonomonas were isolated from diverse soil samples in England and were shown to differ in their morphology, physiology, DNA base composition, molecular genetics, and 16S rDNA sequences from the two marine strains of Marinosulfonomonas, which were isolated from British coastal waters. The marine strains were almost indistinguishable from each other and are considered to be strains of one species. Type species of each genus have been identified and named Methylosulfonomonas methylovora (strain M2) and Marinosulfonomonas methylotropha (strain PSCH4). Phylogenetic analysis using 16S rDNA sequencing places both genera in the α-Proteobacteria. Methylosulfonomonas is a discrete lineage within the α-2 subgroup and is not related closely to any other known bacterial genus. The Marinosulfonomonas strains form a monophyletic cluster in the α-3 subgroup of the Proteobacteria with Roseobacter spp. and some other partially characterized marine bacteria, but they are distinct from these at the genus level. This work shows that the isolation of bacteria with a unique biochemical character, the ability to grow on methanesulfonic acid as energy and carbon substrate, has resulted in the identification of two novel genera of methylotrophs that are unrelated to any other extant methylotroph genera.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK and Boston, USA : Blackwell Publishers Ltd
    Metroeconomica 50 (1999), S. 0 
    ISSN: 1467-999X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: In this paper we study how an elected government maximizes social welfare over its period in office, subject to a growth–equality trade-off. Optimal output and equality paths are derived and their implications are discussed. A central finding is the prediction of political business cycles in both growth and equality. It is also demonstrated that any tightening of the growth–equality trade-off should encourage government to favour growth relative to equality.
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The c-type cytochrome and protein profiles were compared for a number of cultures of Paracoccus denitrificans obtained from a range of culture collections. The cultures fell into two groups corresponding to the two original isolates of this bacterial species. One group, which included NCIMB 8944, ATCC 13543, ATCC 17741, ATCC 19367, Pd 1222 and DSM 413, were similar or identical to LMD 22.21. The second group, including DSM 65 and LMG 4218, were similar or identical to LMD 52.44. These groupings were not compatible with the recorded history of culture deposition. Mass spectrometry and amino acid sequence comparisons showed that the cytochrome c-550 of the LMD 52.44 culture group differed by 16% from that of the LMD 22.21 group, and yet was only 1% different from the cytochrome c-550 of Thiosphaera pantotropha. These results suggest that consideration should be given to creation of a new species of Paracoccus pantotropha, which would include Thiosphaera pantotropha and Paracoccus denitrificans LMD 52.44.
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  • 4
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Leadership & organization development journal 18 (1997), S. 194-200 
    ISSN: 0143-7739
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Suggests that research attention has increasingly focused on the human aspects of mergers and acquisitions (M&A), particularly the problem of socio-cultural integration. Alongside high levels of M & A activity, there has been a growing trend towards the reverse process of demerger and divestment. Like M&As, demerger is a major change process and worthy of research attention. Considers the application of theories and themes developed within the M&A literature to the emerging phenomenon of demerger. Discusses these with reference to the ICI demerger in the early 1990s.
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  • 5
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 1484-1486 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The absolute requirement of Ca2+ for proteolytic activity is a feature unique to the calpains, a family of heterodimeric cysteine proteases. Conditions are described which give rise to diffraction-quality crystals of m-calpain in two crystal forms, P1 and P21. Data have been collected from native crystals of m-calpain in both P1 and P21 forms, to 2.6 and 2.15 Å, respectively. Selenomethionine-containing crystals have been grown in both forms, and anomalous data from the P21 selenomethionine enzyme provided the location of 17 of the 19 Se atoms in the protein.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 29 (1998), S. 0 
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract— Wild-caught, adult summer flounder Paralichthys dentatus (avg. wt. = 740 g; range = 264–1,540 g; N= 60), collected in northeastern US coastal waters during October 1994, were transported to Vero Beach, Florida in March 1995 and held in 2.6-m3 indoor tanks through November 1995 under two artificial photothermal regimes: (1) natural regime, simulating natural habitat conditions; and (2) accelerated thermal regime, with seasonal temperature changes advanced by one month. A third group of fish was held in outdoor tanks under ambient photothermal conditions. Under all photothermal conditions, onset of vitellogenesis was associated with declining daylength and temperature, beginning in the accelerated group, then progressing to the natural and the ambient groups. From 20 September to 28 November 1995, 23 vitellogenic stage females from the accelerated and natural regimes were implanted with a cholesterol-cellulose pellet containing LHRH-a (100 μg/kg body wt). Females with initial mean oocyte diameters ranging from 258–456 μm spawned voluntarily 2.5–5.5 d postimplantation, while no maturational response was obtained from females with mean diameters ranging from 165–231 μm. Two females were spawned twice during the study period by LHRH-a pellet implantation. Infrequent, natural spawning without hormone intervention was also obtained. Females released from 22.7–396.9 × 103 eggs on the first day of spawning, with fertilization and hatching rates of 0–93.470 and 0–81.1%, respectively.
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  • 7
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two 10-day hatchery experiments were conducted to evaluate s-type (Hawaiian strain) and ss-type (Thailand strain) rotifers Brachionus plicatilis and cryogenically preserved oyster Crassostrea gigas trochophores as first feeds for larval Nassau grouper Epinephelus striatus. Newly hatched grouper larvae were reared at densities of 11.2–20.8/L in 500-L tanks at 36–38 ppt salinity, 25–26 C, and under a 11-h light: 13-h dark photoperiod. Beginning on day 2 posthatching (d2ph), prey were maintained at a density of 20 individuals/mL, while phytoplankton (Nanochloropsis oculata) was maintained at 500 × 103 cells/mL. In experiment 1, survival and growth were higher (P 〈 0.05) for fish fed small s-type rotifers (mean lorica length = 117 μm; fish survival = 7.96%) selected by sieving than for fish fed non-selected rotifers (mean lorica length = 161 μm; fish survival = 2.13%). These results demonstrated the advantage of small prey size and suggested that super-small (ss-type) rotifer strains would be beneficial. In experiment 2, three feeding regimens were compared: 1) ss-type rotifers (mean lorica length = 147 μm); 2) oyster trochophores (mean diameter = 50 μm) gradually replaced by ss-type rotifers from d5ph; and 3) a mixed-prey teatment of 50% oyster trochophores and 50% ss-type rotifers. Survival was higher (P 〈 0.05) for larvae fed mixed prey (15.6%) than for those fed rotifers (9.73%) or trochophores and rotifers in sequence (2.55%), which also showed the slowest growth. Oyster trochophores, although inadequate when used exclusively, enhanced survival when used in combination with rotifers, possibly by improving size selectivity and dietary quality. In a pilot-scale trial, larvae were cultured through metamorphosis in two 33.8-m3 outdoor tanks. Fertilized eggs were stocked at a density of 10 eggs/L and larvae were fed ss-type rotifers from d2ph-d20ph, newly hatched Artemia from d15ph-d18ph, 1-d-old Artemia nauplii from d18ph-d62ph. Survival on d62ph was 1.17%, with a total of 5,651 post-metamorphic juveniles produced.
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  • 8
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wild-caught mutton snapper Lutjanus analis, a high-value marine food fish species, matured in flow-through seawater (36 g/L) tanks after 3 yr in captivity. On 31 May 1995, a female with a mean oocyte diameter of 382 μm was injected with human chorionic gonadotropin (HCG) (500 IU/kg body wt.) followed 24 h later by a second injection (1,000 IU/kg body wt.). At the time of the second injection, three males were injected with HCG (500 IU/ kg body wt.). Voluntary spawning occurred 33 h after the first injection, with a total of 534, 781 eggs released. Fertilization rate was 75.7%, while average diameter of fertilized eggs was 783 μm. Embryos were stocked in a 30-m3 outdoor tank at a density of 10.5/L. On day 2 post-hatching (d2ph), larval density was 8.61 larvae/L, and average notochord length was 2.6 mm. Larvae were fed ss-type rotifers from dl-d28ph, Artemia nauplii from 0–08ph, and artificial diets (52–48% protein) from d24-d38ph. On d38ph, fish averaged 0.308 g and 22.2 mm standard length. Survival (from d2ph) was 14.3%, with a total of 36,900 post-metamorphic juveniles produced. On d97ph, 1,390 hatchery-reared juveniles (avg. wt. = 10.5 g) were stocked into two 14.5-m3 recirculating seawater tanks (695 fish/tank; 48 fish/m3) and fed a 56% protein pellet. After 168 d, fish averaged 140.8 g, with a survival rate of 97.8% and a feed conversion ratio (dry wt./wet wt.) of 1.2. These preliminary results reveal the mutton snapper to be a prime, new candidate species for commercial cultivation.
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  • 9
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Entomology 40 (1995), S. 269-295 
    ISSN: 0066-4170
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
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  • 10
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 4348-4357 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: This article presents evidence that cobalt forms a series of optically active defect centers in diamond grown by high-temperature, high-pressure synthesis. Photoluminescence (PL) studies reveal that the newly observed vibronic systems with zero-phonon energies at 1.989, 2.135, 2.207, 2.277, 2.367, and 2.590 eV appear only in samples grown using a cobalt-containing solvent–catalyst. Results of an annealing study, carried out in the temperature range 1500 to 1800 °C, establish that many of the new bands appear during the temperature regime of nitrogen aggregation. It is therefore proposed that nitrogen forms complexes with cobalt to produce optically active centers, in a manner analogous to that of nickel point defects in diamond. Detailed radiative decay time measurements and temperature dependence measurements show that all but one of the bands which are here associated with nitrogen–cobalt complexes have long radiative decay times (∼100 μs), and this again is a characteristic of the PL centers arising from nickel–nitrogen complexes. All of the vibronic bands observed by PL may also be produced by electron-beam excitation (cathodoluminescence). In this case it is necessary to use a low beam current density (≤10 mA cm−2), otherwise the spectra are dominated by emission from optical centers with much shorter decay times (∼20 ns). Only one vibronic band, with a zero-phonon line at 1.852 eV, has been detected in absorption measurements, and the center responsible for this system does not give rise to luminescence. © 1996 American Institute of Physics.
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